scholarly journals Isolation and Transcriptional Regulation of the Fructose 1,6-Bisphosphate Aldolase Gene FBA1 in the Psychrophilic Yeast Cystofilobasidium capitatum

2007 ◽  
Vol 33 (1) ◽  
pp. 9-13
Author(s):  
Shuki FUJIMURA ◽  
Masataka UCHINO ◽  
Takashi ITO ◽  
Takao MYODA ◽  
Toshinori NAGAOKA ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Psychrophilic Yeast ◽  
Fructose 1,6 Bisphosphate ◽  
Aldolase Gene

Isolation and transcriptional regulation of theKluyveromyces lactisFBA1(fructose-1,6-bisphosphate aldolase) gene

2004 ◽  
Vol 50 (8) ◽  
pp. 645-652 ◽  
Author(s):  
Silvia M Díaz Prado ◽  
M Esperanza Cerdán ◽  
M Isabel González Siso
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptional Regulation ◽  
Low Temperature ◽  
Stationary Phase ◽  
Kluyveromyces Lactis ◽  
Reversal Shift ◽  
Aerobic Conditions ◽  
Fermentative Metabolism ◽  
Fructose 1,6 Bisphosphate ◽  
Aldolase Gene

Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is mediated by heme since expression was higher in a mutant defective in heme biosynthesis. KlFBA1 transcription is not induced by calcium-shortage, low temperature, or at stationary phase. These data suggest that KlFBA1 plays a role in the balance between oxidative and fermentative metabolism and that this gene is differentially regulated in K. lactis and Saccharomyces cerevisiae, i.e., a respiratory vs. fermentative yeast.Key words: FBA1, fructose-1,6-bisphosphate aldolase, Kluyveromyces, transcriptional regulation, yeast.

Transcriptional regulation of an aldolase gene in the regenerating rat liver

1990 ◽  
Vol 25 (3) ◽  
pp. 350-355
Author(s):  
Mitsuaki Motomura ◽  
Tsunehiro Mukai ◽  
Iwata Ozaki ◽  
Keiichiro Joh ◽  
Yuji Arai ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Rat Liver ◽  
Regenerating Rat Liver ◽  
Aldolase Gene

scholarly journals Alternate use of divergent forms of an ancient exon in the fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster.

1992 ◽  
Vol 12 (2) ◽  
pp. 773-783 ◽  
Author(s):  
J Kim ◽  
J J Yim ◽  
S Wang ◽  
D Dorsett
Keyword(s):  
Amino Acids ◽  
Drosophila Melanogaster ◽  
Catalytic Domain ◽  
The Third ◽  
Evolutionarily Conserved ◽  
Exon 9 ◽  
Fructose 1,6 Bisphosphate ◽  
A Site ◽  
Aldolase A ◽  
Aldolase Gene

The fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster contains three divergent copies of an evolutionarily conserved 3' exon. Two mRNAs encoding aldolase contain three exons and differ only in the poly(A) site. The first exon is small and noncoding. The second encodes the first 332 amino acids, which form the catalytic domain, and is homologous to exons 2 through 8 of vertebrates. The third exon encodes the last 29 amino acids, thought to control substrate specificity, and is homologous to vertebrate exon 9. A third mRNA substitutes a different 3' exon (4a) for exon 3 and encodes a protein very similar to aldolase. A fourth mRNA begins at a different promoter and shares the second exon with the aldolase messages. However, two exons, 3a and 4a, together substitute for exon 3. Like exon 4a, exon 3a is homologous to terminal aldolase exons. The exon 3a-4a junction is such that exon 4a would be translated in a frame different from that which would produce a protein with similarity to aldolase. The putative proteins encoded by the third and fourth mRNAs are likely to be aldolases with altered substrate specificities, illustrating alternate use of duplicated and diverged exons as an evolutionary mechanism for adaptation of enzymatic activities.

Alternate use of divergent forms of an ancient exon in the fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster

1992 ◽  
Vol 12 (2) ◽  
pp. 773-783
Author(s):  
J Kim ◽  
J J Yim ◽  
S Wang ◽  
D Dorsett
Keyword(s):  
Amino Acids ◽  
Drosophila Melanogaster ◽  
Catalytic Domain ◽  
The Third ◽  
Evolutionarily Conserved ◽  
Exon 9 ◽  
Fructose 1,6 Bisphosphate ◽  
A Site ◽  
Aldolase A ◽  
Aldolase Gene

The fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster contains three divergent copies of an evolutionarily conserved 3' exon. Two mRNAs encoding aldolase contain three exons and differ only in the poly(A) site. The first exon is small and noncoding. The second encodes the first 332 amino acids, which form the catalytic domain, and is homologous to exons 2 through 8 of vertebrates. The third exon encodes the last 29 amino acids, thought to control substrate specificity, and is homologous to vertebrate exon 9. A third mRNA substitutes a different 3' exon (4a) for exon 3 and encodes a protein very similar to aldolase. A fourth mRNA begins at a different promoter and shares the second exon with the aldolase messages. However, two exons, 3a and 4a, together substitute for exon 3. Like exon 4a, exon 3a is homologous to terminal aldolase exons. The exon 3a-4a junction is such that exon 4a would be translated in a frame different from that which would produce a protein with similarity to aldolase. The putative proteins encoded by the third and fourth mRNAs are likely to be aldolases with altered substrate specificities, illustrating alternate use of duplicated and diverged exons as an evolutionary mechanism for adaptation of enzymatic activities.

scholarly journals Effects of the Chloroplast Fructose-1,6-Bisphosphate Aldolase Gene on Growth and Low-Temperature Tolerance of Tomato

2022 ◽  
Vol 23 (2) ◽  
pp. 728
Author(s):  
Bingbing Cai ◽  
Yu Ning ◽  
Qiang Li ◽  
Qingyun Li ◽  
Xizhen Ai
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Chilling Stress ◽  
Low Temperature Stress ◽  
Economic Losses ◽  
Tomato Plants ◽  
Over Expression ◽  
Mda Content ◽  
Fructose 1,6 Bisphosphate ◽  
Aldolase Gene

Tomato (Solanum lycopersicum) is one of the most important greenhouse vegetables, with a large cultivated area across the world. However, in northern China, tomato plants often suffer from low-temperature stress in solar greenhouse cultivation, which affects plant growth and development and results in economic losses. We previously found that a chloroplast aldolase gene in tomato, SlFBA4, plays an important role in the Calvin-Benson cycle (CBC), and its expression level and activity can be significantly altered when subjected to low-temperature stress. To further study the function of SlFBA4 in the photosynthesis and chilling tolerance of tomato, we obtained transgenic tomato plants by the over-expression and RNA interference (RNAi) of SlFBA4. The over-expression of SlFBA4 led to higher fructose-1,6-bisphosphate aldolase activity, net photosynthetic rate (Pn) and activity of other enzymes in the CBC than wild type. Opposite results were observed in the RNAi lines. Moreover, an increase in thousand-seed weight, plant height, stem diameter and germination rate in optimal and sub-optimal temperatures was observed in the over-expression lines, while opposite effects were observed in the RNAi lines. Furthermore, over-expression of SlFBA4 increased Pn and enzyme activity and decreased malonaldehyde (MDA) content under chilling conditions. On the other hand, Pn and MDA content were more severely influenced by chilling stress in the RNAi lines. These results indicate that SlFBA4 plays an important role in tomato growth and tolerance to chilling stress.

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