scholarly journals Amylase and Invertase Activity and Substrate Affinity in 'Danshaku' Potato Tubers (Solanum tuberosum L.) During Cold Storage

2000 ◽  
Vol 26 (5) ◽  
pp. 281-287
Author(s):  
Yosuke MURATA ◽  
Tomohiro NOGUCHI ◽  
Kevin F. YAPTENCO ◽  
Hiroaki SATO ◽  
Shinji MATSUMOTO ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Cold Storage ◽  
Solanum Tuberosum L ◽  
Invertase Activity ◽  
Substrate Affinity ◽  
Potato Tubers

scholarly journals Property Changes in Potato Tubers (Solanum tuberosum L.) During Cold Storage at 0 and 10°C

2000 ◽  
Vol 26 (3) ◽  
pp. 153-160 ◽  
Author(s):  
Yosuke MURATA ◽  
Kevin F. YAPTENCO ◽  
Tomohiro NOGUCHI ◽  
Toshiro SUZUKI ◽  
Hiroaki SATO ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Cold Storage ◽  
Solanum Tuberosum L ◽  
Potato Tubers ◽  
Property Changes

Polyacrylamid-Elektrophorese zur Beurteilung von Proteinen der Kartoffel (Solanum tuberosum L.)

1966 ◽  
Vol 21 (9) ◽  
pp. 879-888 ◽  
Author(s):  
Volkmar Loeschcke ◽  
Hermann Stegemann
Keyword(s):  
Ascorbic Acid ◽  
Solanum Tuberosum ◽  
Pore Size ◽  
Plant Material ◽  
Solanum Tuberosum L ◽  
Protein Pattern ◽  
Potato Tubers ◽  
Active Inhibitor ◽  
Electrophoretic Behavior ◽  
Acidic Proteins

Up to 25 proteins have been found in the sap of potato tubers by polyacrylamid electrophoresis. The mode of preparation and concentration of the plant material was investigated as well as the pretreatment of the polyacrylamid gel to avoid undue retardation of the migrating bands.Sulfite was the most active inhibitor of sap-browning compared with ascorbic acid and cysteine. which did not influence the protein pattern except for one line. The juice had to be dialyzed or filtered through Sephadex G 25 to remove an unkown acidic material which interferes with the more acidic proteins and causes spreading and diffusing of bands. Proteins were concentrated by means of Carbowax MW 20000. For best results ionic contaminations inherent in the gels was removed by electrophoresis before applying the proteins or dyes. Very few proteins were affected by decreasing the pore size of the gel. Ammonium sulfate (40% saturation) precipitated mainly those proteins which are similar in electrophoretic behavior to the albumin- and transferrinregion of human plasma.

scholarly journals Lipoxygenase Pathway and Membrane Permeability and Composition during Storage of Potato Tubers (Solanum tuberosum L. cv Bintje and Désirée) in Different Conditions

Plant Biology ◽  
2002 ◽  
Vol 4 (1) ◽  
pp. 77-85 ◽  
Author(s):  
M.-L. Fauconnier ◽  
J. Rojas-Beltrán ◽  
J. Delcarte ◽  
F. Dejaeghere ◽  
M. Marlier ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Membrane Permeability ◽  
Solanum Tuberosum L ◽  
Potato Tubers ◽  
Lipoxygenase Pathway

RNAi effects on regulation of endogenous acid invertase activity in potato (Solanum tuberosum L.) tubers

2008 ◽  
Vol 5 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Zhang Chi ◽  
Xie Cong-Hua ◽  
Song Bo-Tao ◽  
Liu Xun ◽  
Liu Jun
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Pcr Amplification ◽  
Invertase Activity ◽  
Northern Blotting ◽  
Acid Invertase ◽  
Transcriptional Gene Silencing ◽  
Post Transcriptional Gene Silencing ◽  
Transgenic Lines ◽  
Potential Strategy

AbstractIn plants, acid invertases are known to be the key enzymes cleaving sucrose into reducing sugars (RS) (glucose and fructose). To improve the quality of potato (Solanum tuberosum L.) chips, which is largely influenced by RS accumulation in tubers stored at low temperature, a part of acid invertase cDNA with hairpin RNA (hpRNA) structure was transformed into potato cv. N2. Detection of polymerase chain reaction (PCR) amplification and Northern blotting suggested that the RNA interference (RNAi) vector was successfully transformed into cv. N2. The analysis of acid invertase activity in the plantlets and microtubers of RNAi transgenic lines indicated that the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets by an average 69.8% (with the exception of line Ni-1) with a maximal decrease of 78% (line Ni-4), and the highest decrease of activity in microtubers of 68%. Compared with that of well-inhibited antisense inv transgenic plants, the comparative downregulation of RNAi suggests a distinct alteration of endogenous acid invertase activity and a potential strategy for post-transcriptional gene silencing (PTGS) in modulation of cold-sweetening in potato.

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