scholarly journals Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species

2016 ◽  
Author(s):  
Jelena Ačanski ◽  
Ante Vujić ◽  
Mihajla Djan ◽  
Dragana Obreht Vidaković ◽  
Gunilla Ståhls ◽  
...  
Keyword(s):  
Sibling Species ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Coi Gene ◽  
Species Boundaries ◽  
Geometric Morphometric ◽  
Molecular Analyses ◽  
Mtdna Coi ◽  
The Status ◽  
Coi Sequences

Several recent studies have detected and described complexes of cryptic and sibling species in the genus Merodon (Diptera, Syrphidae). One representative of these complexes is the Merodon avidus complex that contains four sibling species, which have proven difficult to distinguish using traditional morphological characters. In the present study, we use two geometric morphometric approaches, as well as molecular characters of the 5’-end of the mtDNA COI gene, to delimit sibling taxa. Analyses based on these data were used to strengthen species boundaries within the complex, and to validate the status of a previously-recognized cryptic taxon from Lesvos Island (Greece), here described as Merodon megavidus Vujić & Radenković sp. nov. Geometric morphometric results of both wing and surstylus shape confirm the present classification for three sibling species－M. avidus (Rossi, 1790), M. moenium Wiedemann in Meigen, 1822 and M. ibericus Vujić, 2015－and, importantly, clearly discriminate the newly-described taxon Merodon megavidus sp. nov. In addition to our geometric morphometric results, supporting characters were obtained from molecular analyses of mtDNA COI sequences, which clearly differentiated M. megavidus sp. nov. from the other members of the M. avidus complex. Molecular analyses revealed that the earliest divergence of M. ibericus occurred around 800 ky BP, while the most recent separation happened between M. avidus and M. moenium around 87 ky BP.

scholarly journals Integrative Taxonomy of Two Peruvian Strains of Brachionus plicatilis Complex with Potential in Aquaculture

Diversity ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 671
Author(s):  
Pedro Pablo Alonso Sánchez-Dávila ◽  
Giovanna Sotil ◽  
Araceli Adabache-Ortiz ◽  
Deivis Cueva ◽  
Marcelo Silva-Briano
Keyword(s):  
Egg Production ◽  
Brachionus Plicatilis ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Reproductive Age ◽  
Coi Gene ◽  
Phylogenetic Position ◽  
Mtdna Coi ◽  
The Relationship

Two Peruvian strains of the genus Brachionus were isolated from impacted coastal wetlands. With an integrative taxonomic view, we described their taxonomic status, morphological characters, productive parameters, and phylogenetic position. In the case of both strains, the relationship between biometrics and productive parameters obtained with Principal Components Analysis indicated that the lorica length was associated with longevity, progeny, egg production, and reproductive age, while the lorica width and aperture were associated with the maximum number of eggs carried. Maximum Likelihood and Bayesian Inference analysis carried out with mtDNA COI gene and rDNA ITS1 region showed that both strains were clustered in two clades with distinct phylogenetic positioning from what is currently known for Brachionus plicatilis s.l. One of the strains, Z010-VL, is proposed to be a subspecies of L4 (B. paranguensis), and the other strain, Z018-SD, is proposed as a sub species of SM2 (B. koreanus). In addition, 33 and 31 aquaculture production lineages are proposed, delimited by COI and concatenated COI+ITS1 sequences, respectively. Finally, this study provides new tools that enhance the traceability of the origin of each sub-species throughout the world.

Taxonomic reassessment of the freshwater mussel genus Unio (Bivalvia: Unionidae) in Russia and Ukraine based on morphological and molecular data

Zootaxa ◽  
2017 ◽  
Vol 4286 (1) ◽  
pp. 93 ◽  
Author(s):  
OLGA KLISHKO ◽  
MANUEL LOPES-LIMA ◽  
ELSA FROUFE ◽  
ARTHUR BOGAN ◽  
LYUDMILA VASILIEV ◽  
...  
Keyword(s):  
Freshwater Mussel ◽  
Integrated Approach ◽  
Molecular Data ◽  
Coi Gene ◽  
Data Set ◽  
Widespread Species ◽  
Molecular Analyses ◽  
Morphological Variations ◽  
Mtdna Coi ◽  
Morphological And Molecular Data

The taxonomy of species within the genus Unio (Bivalvia: Unionidae: Unioninae) in Russia and Ukraine has been contentious due to the lack of correspondence between three concurrent yet divergent classifications. In order to clarify which classification system best reflects the evolutionary relationships among these taxa, we performed detailed morphological analyses on 720 Ukrainian and Russian specimens, complemented with molecular data (COI) from a selected number of specimens. The morphological character data set shows the existence of only three widespread species with slight eco-morphological variations. Statistical analyses of shell morphometric parameters and molecular analyses based on mtDNA COI gene fragment sequences confirm the existence of the same three species within a single genus, Unio, in Russia and Ukraine, that is U. pictorum, U. tumidus and U. crassus. Results from molecular analyses suggest the existence of an additional subgroup within the U. crassus lineage, U. crassus cf. courtilieri that deserves further research. The present integrated approach confirms the validity of the classification by Zhadin (1952) and rejects the complex classifications of Starobogatov et al. (2004) and Bogatov & Kijashko (2016). 

scholarly journals Perlidae (Plecoptera) from the Paranapiacaba Mountains, Atlantic Forest, Brazil: Diversity and implications of the integrative approach and teneral specimens on taxonomy

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243393
Author(s):  
Lucas Henrique de Almeida ◽  
Pitágoras da Conceição Bispo
Keyword(s):  
New Record ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Coi Gene ◽  
Southeastern Brazil ◽  
Integrative Approach ◽  
South American ◽  
Neighbor Joining ◽  
Previous Record ◽  
Coi Sequences

The study of complementary sources of biological variation (e.g. morphological, molecular) has allowed a better understanding of biodiversity through the construction of an integrative taxonomy. Using this approach, specimens from the Paranapiacaba Mountains, southeastern Brazil, were studied to update the knowledge on the stonefly family Perlidae from the region, characterize the species, and make associations between nymphs and adults using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene. The study also discusses the implications of integrative taxonomy and teneral specimens for the study of South American Perlidae. The molecular data were analyzed using Bayesian inference, Neighbor-joining, and delimiting species methods. Our results revealed that, in general, there was a morphological and molecular congruence between species. In the Paranapiacaba Mountains, three genera and 15 species were recorded: Anacroneuria boraceiensis Froehlich 2004, A. debilis (Pictet 1841) (new record), A. fiorentini De Ribeiro and Froehlich 2007 (new record), A. flintorum Froehlich 2002, A. iporanga Bispo and Froehlich 2004, A. itajaimirim Bispo and Froehlich 2004, A. polita (Burmeister 1913), A. subcostalis Klapálek 1921, A. tupi Bispo and Froehlich 2004 (with a description of the nymph), Kempnyia auberti Froehlich 1996, K. colossica (Navás 1934), K. flava Klapálek 1916, K. neotropica (Jacobson and Bianchi 1905) (including its new junior synonym K. petersorum Froehlich 1996), Kempnyia sp., and Macrogynoplax veneranda Froehlich 1984. COI sequences were obtained for 11 species, five of which had nymphs associated with adults. Among the five associated nymphs, the nymph of A. tupi is described here. The results of this study indicate that the color of adult teneral specimens differs from that of mature specimens. Given this, the synonym of K. neotropica and K. petersorum was proposed since these species have high morphological and molecular similarities and differ only in color patterns. In addition, the previous record of A. petersi Froehlich 2002 from the Paranapiacaba Mountains was invalidated since it was considered a teneral specimen of A. flintorum. These results suggest that the development of an integrative taxonomy is essential to continue advancing the study of Perlidae diversity in South America.

scholarly journals Cave shrimps Troglocaris s. str. (Dormitzer, 1853), taxonomic revision and description of new taxa after phylogenetic and morphometric studies

Zootaxa ◽  
2012 ◽  
Vol 3421 (1) ◽  
pp. 1 ◽  
Author(s):  
JURE JUGOVIC ◽  
BRANKO JALŽIĆ ◽  
SIMONA PREVORČNIK ◽  
BORIS SKET
Keyword(s):  
Type Species ◽  
Morphological Variability ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Molecular Data ◽  
Coi Gene ◽  
Distribution Area ◽  
Molecular Analyses ◽  
Morphometric Analyses ◽  
Morphological Distinction

Within the Dinaric genus Troglocaris cave shrimps from the subgenus Troglocaris s. str. (Dormitzer, 1853) (Crustacea:Decapoda: Atyidae), have the widest distribution area. The recent molecular analyses have revealed significant, crypticdiversity in the subgenus. The aim of the subsequent detailed morphometric analyses was the provision of the appropriatediagnosable characters for the discovered lineages, i.e. taking care of their taxonomical visibility. We herein designate aneotype and provide a detailed description for the polytipic type species of the genus T. (T.) anophthalmus (Kollar, 1848), toenable its morphological distinction from the erroneously described T. (T.) planinensis Birštejn, 1948. Considering acombination of morphological, geographical and molecular data, we describe four new subspecies: T. (T.) a. ocellata ssp. nov.,T. (T.) a. periadriatica ssp. nov., T. (T.) a. legovici ssp. nov. and T. (T.) a. sontica ssp. nov., apart from the extant T. (T.) a.intermedia Babić, 1922. Due to a considerable morphological variability and no easily observable diagnostic morphological characters, the GenBank accession numbers for the COI gene are added in all mentioned taxa.

scholarly journals Molecular Identification and Geometric Morphometric Analysis of Haematobosca aberrans (Diptera: Muscidae)

Insects ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 451 ◽  
Author(s):  
Tanasak Changbunjong ◽  
Jiraporn Ruangsittichai ◽  
Gerard Duvallet ◽  
Adrian C. Pont
Keyword(s):  
Sexual Dimorphism ◽  
Geometric Morphometrics ◽  
Morphometric Analysis ◽  
Morphological Characters ◽  
Wing Shape ◽  
Coi Gene ◽  
Morphological Identification ◽  
Data Systems ◽  
Geometric Morphometric ◽  
Geometric Morphometric Analysis

The genus Haematobosca Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020. In the present study, the mitochondrial cytochrome c oxidase I (COI) gene was used to confirm the morphological identification of H. aberrans. In addition, landmark-based geometric morphometrics was used to determine sexual dimorphism. The molecular analysis was conducted with 10 COI sequences. The results showed that all sequences were 100% identical. The sequence was not highly similar to reference sequences from GenBank and did not match any identified species from Barcode of Life Data Systems (BOLD). Phylogenetic analysis clearly differentiated this species from other species within the subfamily Stomoxyinae. For geometric morphometric analysis, a total of 16 wing pictures were analysed using the landmark-based approach. The results showed significant differences in wing shape between males and females, with a cross-validated classification score of 100%. The allometric analysis showed that wing shape has no correlation with size. Therefore, the COI gene is effective in species identification of H. aberrans, and geometric morphometrics is also effective in determining sexual dimorphism.

scholarly journals Molecular Characterization of Anopheles sacharovi Based on Sequences of ITS2-rDNA Region and COI Gene in North of Iran

2019 ◽  
Author(s):  
Sahereh Gholami ◽  
Hasan Bakhshi ◽  
Seyyed Hassan Moosa-Kazemi ◽  
Alireza Zahraei-Ramazani ◽  
Alireza Chavshin ◽  
...  
Keyword(s):  
Sibling Species ◽  
Coi Gene ◽  
Its2 Region ◽  
Morphological Identification ◽  
Anopheles Mosquitoes ◽  
North Of Iran ◽  
Northwest Of Iran ◽  
Coi Sequences ◽  
West Azerbaijan ◽  
East Azerbaijan

Background: Malaria is an important mosquito-borne disease considered as one of the public health concerns across many countries. Anopheles mosquitoes are the main vectors of Plasmodium parasites, which cause malaria. Some of these vectors such as Anopheles maculipennis s.l. and Anopheles sacharovi are considered as complex of sibling species distributed in north of Iran. Methods: This study was conducted in north and northwest of Iran including East Azerbaijan, West Azerbaijan, Ar­dabil, Golestan and North Khorasan provinces with emphasis on the northern borders of the country during 2015–2016. Adult specimens were collected and subjected to morphological identification as well as molecular analysis. Results: Overall, 10405 mosquitoes were collected comprising 21 species. Culex pipiens and Cx. theileri were found as the most frequent species in whole study area. Morphological identification showed that out of 1455 female Anoph­eles specimens, 77% belonged to An. maculipennis Group. Out of the identified species, ITS2 region and COI gene sequences of 8 An. maculipennis s.s. and 31 An. sacharovi representing all provinces were obtained and submitted to GenBank. The COI sequences for An. sacharovi revealed the presence of 9 haplotypes with similarity of 98.17–100%. Conclusion: Some investigations have reported An. martinius as a member of sibling species of An. sacharovi among Iranian Anopheles genus; while based on our study, there was no evidence of the presence of this species in north and northwest of Iran.  

scholarly journals Integrative taxonomy supports the presence of two species of Kyphosus (Perciformes: Kyphosidae) in Atlantic European waters

2017 ◽  
Vol 81 (4) ◽  
pp. 467 ◽  
Author(s):  
Rafael Bañón ◽  
David Barros-García ◽  
Alejandro De Carlos
Keyword(s):  
New Record ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Integrative Taxonomy ◽  
Index Number ◽  
Dna Barcodes ◽  
Northwestern Spain ◽  
Kyphosus Vaigiensis ◽  
Coi Sequences ◽  
Public Repositories

The taxonomic identification of one Kyphosus sectatrix and two Kyphosus vaigiensis specimens caught in the European Atlantic waters of Galicia, northwestern Spain, was carried out by means of integrative taxonomy, combining the examination of morphological characters and DNA barcodes. Taxonomical assignation based on morphological characters of these specimens was tested by comparing their COI sequences with available data of Kyphosus deposited in public repositories. The resulting neighbour-joining tree defined four clades corresponding to Barcode Index Number (BIN) and indicated that some nucleotide sequences from Kyphosus, previously deposited, probably originate from misidentified specimens, as would be expected from cryptic and sympatric species. The specimens of Kyphosus vaigiensis represent a new record for the waters of Galicia and the northernmost record in the eastern Atlantic. This kind of herbivorous tropical fishes could play an important role in the tropho-dynamic context of this temperate coastal ecosystem.

scholarly journals The first molecular insight into the genus Turanium Baeckmann, 1922 (Coleoptera: Cerambycidae: Callidiini) with a description of a new species from Middle Asia

2021 ◽  
Vol 79 ◽  
pp. 465-484
Author(s):  
Lech Karpiński ◽  
Wojciech T. Szczepański ◽  
Radosław Plewa ◽  
Lech Kruszelnicki ◽  
Katarzyna Koszela ◽  
...  
Keyword(s):  
New Species ◽  
Strong Support ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Central Asian ◽  
Ecological Associations ◽  
Coi Sequences ◽  
A New Species ◽  
Insight Into ◽  
Key Characters

This paper sheds the first light on the phylogeny of the Central Asian genus Turanium Baeckmann, 1922. By applying an integrative taxonomy approach, we revealed and described a new species from Kyrgyzstan—Turanium losi Karpiński, Plewa & Hilszczański sp. nov. Distinguishing characters from closely related Turanium pilosum (Reitter, 1891) are presented and their ecological associations are discussed. The key characters, including the male terminalia, were examined by means of scanning electron microscopy. High-quality stacked photographs of the habitus of the specimens are presented for both species and their geographical distributions are mapped. While the new species shows stable morphological characters that allow its differentiation from T. pilosum and the COI genetic distance between them is approx. 3%, the different species delimitation methods gave discordant results. Although the new species remained unrecognized for so long, it seems that these cerambycids are common in the region and both can be considered potentially invasive as they are apparently highly polyphagous. It has also been documented that they occur sympatrically in Kyrgyzstan. Both the Bayesian and maximum likelihood analyses of COI sequences confirmed the monophyly of the genus Turanium with strong support (PP 1 and BS 90, respectively). Moreover, the recently revealed polyphyly of the tribe Callidiini was supported by our analyses and, consequently, the discussion on the establishment of a new tribe Ropalopini is raised. This study further corroborates the effectiveness of DNA barcoding as a tool in detecting new species and provides some of the first sequences for Central Asian cerambycids, which remain almost completely unknown in terms of molecular studies.

scholarly journals Assessing species boundaries in the open sea: an integrative taxonomic approach to the pteropod genus Diacavolinia

2019 ◽  
Vol 187 (4) ◽  
pp. 1016-1040 ◽  
Author(s):  
Alice K Burridge ◽  
Remy Van Der Hulst ◽  
Erica Goetze ◽  
Katja T C A Peijnenburg
Keyword(s):  
Integrative Taxonomy ◽  
Species Boundaries ◽  
Shell Shape ◽  
Shape Variation ◽  
Geometric Morphometric ◽  
Integrative Framework ◽  
Rdna Sequences ◽  
Biogeographic Provinces ◽  
Taxonomic Approach ◽  
Species Specific

Abstract To track changes in pelagic biodiversity in response to climate change, it is essential to accurately define species boundaries. Shelled pteropods are a group of holoplanktonic gastropods that have been proposed as bio-indicators because of their vulnerability to ocean acidification. A particularly suitable, yet challenging group for integrative taxonomy is the pteropod genus Diacavolinia, which has a circumglobal distribution and is the most species-rich pteropod genus, with 24 described species. We assessed species boundaries in this genus, with inferences based on geometric morphometric analyses of shell-shape variation, genetic (cytochrome c oxidase subunit I, 28S rDNA sequences) and geographic data. We found support for a total of 13 species worldwide, with observations of 706 museum and 263 freshly collected specimens across a global collection of material, including holo‐ and paratype specimens for 14 species. In the Atlantic Ocean, two species are well supported, in contrast to the eight currently described, and in the Indo‐Pacific we found a maximum of 11 species, partially merging 13 of the described species. Distributions of these revised species are congruent with well-known biogeographic provinces. Combining varied datasets in an integrative framework may be suitable for many diverse taxa and is an important first step to predicting species-specific responses to global change.

Baetis bundyae(Ephemeroptera: Baetidae), described from Arctic Canada is found in northernmost Europe

2014 ◽  
Vol 146 (6) ◽  
pp. 621-629 ◽  
Author(s):  
Eino Savolainen ◽  
Marcus K. Drotz ◽  
Anssi Saura ◽  
Gunilla Ståhls
Keyword(s):  
North America ◽  
Distributional Pattern ◽  
Arctic Canada ◽  
Mtdna Coi ◽  
The Status ◽  
Coi Sequences ◽  
Western Palaearctic

AbstractThe taxonomy and identification of mayflies of theBaetis vernusgroup (Ephemeroptera: Baetidae) represents a major challenge in both Europe and North America. The recent description ofB. jaerviiSavolainen from Finland, a species taxonomically close to the Palaearctic taxonBaetis macaniKimmins and the NearcticB. bundyaeLehmkuhl, called for clarification of the status and distribution of these species in northernmost Europe. We generated mtDNA COI sequences for establishing the identity of the collected samples. Based on these data we conclude thatB. bundyaedoes occur in northeastern Finland in sympatry withB. macani. Accordingly, this taxon shows a highly interesting distributional pattern across the Nearctic and western Palaearctic regions.

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