scholarly journals Purification of Pig Muscle Stem Cells Using Magnetic-Activated Cell Sorting (MACS) Based on the Expression of Cluster of Differentiation 29 (CD29)

2020 ◽  
Vol 40 (5) ◽  
pp. 852-859
Author(s):  
Kwang-Hwan Choi ◽  
Minsu Kim ◽  
Ji Won Yoon ◽  
Jinsol Jeong ◽  
Minkyung Ryu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Sorting ◽  
Muscle Stem Cells ◽  
Pig Muscle ◽  
Cluster Of Differentiation ◽  
Magnetic Activated Cell Sorting

scholarly journals Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells†

2019 ◽  
Vol 102 (1) ◽  
pp. 220-232 ◽  
Author(s):  
Hiroko Morimoto ◽  
Mito Kanatsu-Shinohara ◽  
Kyle E Orwig ◽  
Takashi Shinohara
Keyword(s):  
Stem Cells ◽  
Cell Sorting ◽  
Type A ◽  
Spermatogonial Stem Cells ◽  
Fibroblast Growth Factor 2 ◽  
Self Renewal ◽  
Spermatogonial Transplantation ◽  
Short Hairpin ◽  
Functional Analyses ◽  
Magnetic Activated Cell Sorting

Abstract Spermatogonial stem cells (SSCs) undergo continuous self-renewal division in response to self-renewal factors. The present study identified ephrin type-A receptor 2 (EPHA2) on mouse SSCs and showed that supplementation of glial cell-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2), which are both SSC self-renewal factors, induced EPHA2 expression in cultured SSCs. Spermatogonial transplantation combined with magnetic-activated cell sorting or fluorescence-activated cell sorting also revealed that EPHA2 was expressed in SSCs. Additionally, ret proto-oncogene (RET) phosphorylation levels decreased following the knockdown (KD) of Epha2 expression via short hairpin ribonucleic acid (RNA). Although the present immunoprecipitation experiments did not reveal an association between RET with EPHA2, RET interacted with FGFR2. The Epha2 KD decreased the proliferation of cultured SSCs and inhibited the binding of cultured SSCs to laminin-coated plates. The Epha2 KD also significantly reduced the colonization of testis cells by spermatogonial transplantation. EPHA2 was also expressed in human GDNF family receptor alpha 1-positive spermatogonia. The present results indicate that SSCs express EPHA2 and suggest that it is a critical modifier of self-renewal signals in SSCs.

Isolation of lung multipotent stem cells using a novel microfluidic magnetic activated cell sorting system

2015 ◽  
Vol 39 (11) ◽  
pp. 1348-1353 ◽  
Author(s):  
Ling Zeng ◽  
Lin Qiu ◽  
Xue-Tao Yang ◽  
Yin-Han Zhou ◽  
Juan Du ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Sorting ◽  
Multipotent Stem Cells ◽  
Sorting System ◽  
Magnetic Activated Cell Sorting

Enrichment of epidermal stem cells of rats by Vario magnetic activated cell sorting system

2013 ◽  
Vol 49 (8) ◽  
pp. 583-588 ◽  
Author(s):  
Wei Chen ◽  
Wei-wei Zhang ◽  
Chunying Shi ◽  
Xiaohua Lian ◽  
Shanghong Yi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Sorting ◽  
Epidermal Stem Cells ◽  
Sorting System ◽  
Magnetic Activated Cell Sorting

Analysis of the hematopoietic potential of muscle-derived cells in mice

Blood ◽  
2002 ◽  
Vol 100 (2) ◽  
pp. 721-723 ◽  
Author(s):  
Hartmut Geiger ◽  
Jarrod M. True ◽  
Barry Grimes ◽  
Elizabeth J. Carroll ◽  
Roger A. Fleischman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Muscle Tissue ◽  
Cell Sorting ◽  
Hematopoietic Stem ◽  
Hematopoietic System ◽  
Muscle Stem Cells ◽  
Antibody Staining ◽  
Stem And Progenitor Cells

Abstract Cells in murine muscle have been reported to differentiate into hematopoietic stem and progenitor cells and thus repopulate the hematopoietic system of an irradiated animal. This activity was attributed to muscle stem cells. We used an in vitro and in vivo approach to identify the hematopoietic repopulating activity found in muscle tissue of mice by antibody staining and cell sorting. We confirmed existence of a hematopoietic repopulating cell in muscle tissue, but the data strongly suggest that repopulation is due not to muscle stem cells but to hematopoietic cells present in muscle tissue. Unexpectedly, the blood-forming cells were enriched in muscle relative to their frequency in peripheral blood.

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