scholarly journals Evaluation of Cariogenic Potential of Sugar and Sugar Substitutes by in vitro Enamel Demineralization.

1994 ◽  
Vol 44 (1) ◽  
pp. 85-96
Author(s):  
Nobuhiro TAGUCHI
Keyword(s):  
Sugar Substitutes ◽  
Enamel Demineralization

The Potential of a Bioactive, Pre-reacted, Glass-Ionomer Filler Resin Composite to Inhibit the Demineralization of Enamel in Vitro

2021 ◽  
Vol 46 (1) ◽  
pp. E11-E20
Author(s):  
IF Leão ◽  
N Araújo ◽  
CK Scotti ◽  
RFL Mondelli ◽  
MM de Amoêdo Campos Velo ◽  
...  
Keyword(s):  
Resin Composite ◽  
Surface Hardness ◽  
Composition Analysis ◽  
Glass Ionomer ◽  
Material Interface ◽  
Enamel Demineralization ◽  
Restorative Materials ◽  
Ph Cycling ◽  
Group B

Clinical Relevance A prereacted, glass-ionomer filler fluoride-containing resin composite had lower remineralization potential than glass-ionomer cements but was able to inhibit enamel demineralization; thus, it may be an option for restoring dental surfaces for patients at high risk of caries. SUMMARY Evidence is lacking on the use of surface prereacted glass-ionomer filler resin composites to inhibit demineralization and that simulate real clinical conditions. The present laboratory study evaluated the potential of such composites to prevent demineralization and quantified fluoride (F) and other ions released from restorative materials after a dynamic pH-cycling regimen applied to the tooth material interface in vitro. The pH-cycling regimen was assessed by measuring surface hardness (SH) along with energy dispersive X-ray spectroscopy (EDX). Methods and Materials: Ninety blocks of bovine enamel were subjected to composition analysis with EDX, and were further categorized based on SH. The blocks were randomly divided into 6 treatment groups (n=15 each): F IX (Fuji IX Extra; GC Corporation); IZ (Ion Z, FGM); F II (Fuji II LC, GC Corporation); B II (Beautifil II, Shofu); F250 (Filtek Z250 XT, 3M ESPE); and NT (control, no treatment). The blocks were subjected to a dynamic pH-cycling regimen at 37°C for 7 days concurrently with daily alternations of immersion in demineralizing/remineralizing solutions. EDX was conducted and a final SH was determined at standard distances from the restorative materials (150, 300, and 400 μm). Results: The EDX findings revealed a significant increase in F concentration and a decrease in Ca2+ in the enamel blocks of group B II after the pH-cycling regimen (p<0.05). SH values for groups F IX, IZ, and F II were greater than those for groups B II, F250, and NT at all distances from the materials. Conclusions: The results suggest that each of 3 restorative materials, F IX, IZ, and F II, partially inhibited enamel demineralization under a dynamic pH-cycling regimen.

In vitro inhibition of enamel demineralization by a polymerizable amphiphilic film

2000 ◽  
Vol 108 (6) ◽  
pp. 564-568 ◽  
Author(s):  
Daranee Tantbirojn ◽  
Sharon M. Rozzi ◽  
Sumita B. Mitra ◽  
Brant L. Kedrowski ◽  
William H. Douglas
Keyword(s):  
Enamel Demineralization ◽  
In Vitro Inhibition

scholarly journals Comparative assessment of resistance to enamel demineralization after orthodontic banding with three different cements- An in vitro study.

2014 ◽  
Vol 17 (2) ◽  
pp. 159
Author(s):  
Ashwith B Hegde ◽  
Tushar Patil ◽  
Sonali Khandekar ◽  
Gaurav Gupta ◽  
U.S.Krishna Nayak
Keyword(s):  
In Vitro Study ◽  
Comparative Assessment ◽  
Vitro Study ◽  
Enamel Demineralization

Effect of Whole Saliva Components on Enamel Demineralization In Vitro

1993 ◽  
Vol 4 (3) ◽  
pp. 357-362 ◽  
Author(s):  
J.D.B. Featherstone ◽  
J.M. Behrman ◽  
J.E. Bell
Keyword(s):  
Dental Enamel ◽  
Acid Resistance ◽  
Human Teeth ◽  
Cross Sectional ◽  
Basic Proteins ◽  
Enamel Demineralization ◽  
Whole Saliva ◽  
Flow Through ◽  
Mineral Loss

The aim of the present study was to use an in vitro enamel demineralization model (1) to confirm that whole saliva pretreatment conferred acid resistance to dental enamel and (2) to determine whether this phenomenon was attributable to specific salivary proteins, minerals, lipids, or some combination of these. Crowns of human teeth, each with one exposed window, were prepared in groups of ten. They were each pretreated by immersion individually in 4 ml of either (1) clarified whole saliva for 18, 72, or 168 h, (2) dialyzed saliva (3500 MWCO membrane), (3) the "flow-through" fraction from a DEAE separation of whole saliva (neutral and basic proteins), (4) the "eluted" fraction of a DEAE separation of whole saliva (anionic proteins), or (5) a combination of salivary lipids and the DEAE "flow-through" fraction of whole saliva (neutral and basic proteins). Control groups were group 6 with no pretreatment, group 7 pretreated for 168 h in a borate buffer (5 mmol/1), and group 8 pretreated in a mineral solution containing calcium (0.7 mmol/1) and phosphate (2.6 mmol/1). The crowns were then demineralized for 7 d in vitro (0.1 mol/1 acetate, 1 mmol/l Ca and phosphate, pH 5.0) to produce artificial caries-like lesions. Lesions were assessed by cross-sectional microhardness profiles, and mineral loss (AZ, μm x vol% mineral) calculated. Mineral loss (AZ) values decreased linearly with the square root of time of pretreatment by whole saliva, confirming a time-dependent protective effect of salivary pellicle against demineralization of enamel. Pretreatments (168 h) by whole saliva (group 1), dialyzed saliva (group 2), and lipid/'flow through" proteins (group 5) gave equivalent protection (approximately 55%). However, no protection was provided by DEAE-separated protein fractions (no lipid present) or by the mineral alone. The protection of surface enamel against demineralization appears to be given by a combination of specifically adsorbed salivary lipids and proteins.

In-vitro quantitative evaluation of the effectiveness of different techniques on the of incipient enamel demineralization

2017 ◽  
Vol 14 (1) ◽  
pp. 30
Author(s):  
SaraA Gamea ◽  
WedadM Etman ◽  
AliI Abdalla ◽  
HusseinI Saudi
Keyword(s):  
Quantitative Evaluation ◽  
Enamel Demineralization

scholarly journals Reliability analysis of two methods for measuring active enamel demineralization: An in vitro study

2013 ◽  
Vol 07 (02) ◽  
pp. 159-164 ◽  
Author(s):  
Camila Nassur ◽  
Luciana Pomarico ◽  
Lucianne Cople Maia
Keyword(s):  
Image Analysis ◽  
Intraclass Correlation ◽  
In Vitro Study ◽  
Analysis Software ◽  
Computerized Image Analysis ◽  
Enamel Demineralization ◽  
Significant Difference ◽  
Digital Caliper ◽  
Excellent Reproducibility

ABSTRACT Objective: The aim was to evaluate the intra- and inter-examiner reliability of two methods, namely, digital caliper [DC] and computerized image analysis software (Image Tool [IT] version 4.1) for measuring the area of an active enamel demineralization lesion. The effect on that measurement of a window that delimits the lesion was also assessed. Materials and Methods: Thirty-eight circular artificial enamel demineralization lesions (r = 2 mm) were produced on bovine teeth in vitro, which were then measured three times by three examiners, with and without the presence of a window delimiting the area to be measured. The data were analyzed by the intraclass correlation coefficient (ICC) with 95% confidence interval (CI) and by the Altman and Bland analysis. Results: The intra-examiner correlation using the IT method showed excellent reproducibility (mean ICC values 0.922-0.970 with the delimiting window and 0.915-0.990 without the window). However, the intra-examiner correlation using the DC method showed comparatively less reproducibility in the measurements (mean ICC values 0.458-0.648 with the delimiting window and 0.378-0.665 without the window). The inter-examiner correlations showed very good reproducibility of the measurements regardless of the presence or absence of the window for both the DC method (0.811-0.846) and IT method (0.953-0.994). However, the latter method showed less variability within the measurements. Conclusion: However, statistically no significant difference was found between both methodologies. Nevertheless, computerized image analysis with the Image Tool software demonstrated higher intra- and inter-examiner reliability than the digital caliper method, in estimating the area of the enamel demineralization lesion, regardless of the delimiting window.

Characterization of a new TiF4 and β-cyclodextrin inclusion complex and its in vitro evaluation on inhibiting enamel demineralization

2013 ◽  
Vol 58 (3) ◽  
pp. 239-247 ◽  
Author(s):  
Camila Nassur ◽  
Adílis Kalina Alexandria ◽  
Luciana Pomarico ◽  
Valeria Pereira de Sousa ◽  
Lúcio Mendes Cabral ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
In Vitro Evaluation ◽  
Enamel Demineralization ◽  
Cyclodextrin Inclusion ◽  
Cyclodextrin Inclusion Complex

scholarly journals Efficiency of Amorphous Calcium Phosphate–Containing Orthodontic Composite and Resin Modified Glass Ionomer on Demineralization Evaluated By a New Laser Fluorescence Device

2009 ◽  
Vol 03 (02) ◽  
pp. 127-134 ◽  
Author(s):  
Tancan Uysal ◽  
Mihri Amasyali ◽  
Alp Erdin Koyuturk ◽  
Deniz Sagdic
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Glass Ionomer Cement ◽  
Laser Fluorescence ◽  
Orthodontic Brackets ◽  
Glass Ionomer ◽  
Enamel Demineralization ◽  
Significant Difference ◽  
Resin Modified Glass Ionomer

ABSTRACTObjectives: The aim of this in vitro study was to compare the efficacy of Amorphous Calcium Phosphate (ACP)-containing orthodontic composite and resin-modified glass ionomer cement (RMGIC) on enamel demineralization adjacent to orthodontic brackets evaluated by a new laser fluorescence device.Methods: Sixty extracted maxillary premolars were used in the present study. Twenty orthodontic brackets were bonded with ACP-containing orthodontic adhesive (Aegis-Ortho), 20 were bonded with RMGIC (Fuji Ortho LC) ad20 were bonded with Transbond XT composite as the control. All samples were then cycled for 21 days through a daily procedure of demineralization for 6 hours and remineralization for 17 hours. After this procedure, demineralization evaluations were undertaken by a pen-type laser fluorescence device (DIAGNO-dent Pen). Analysis ofvariance (ANOVA) and Tukey test was used for statistical evaluation, at P<.05 level.Results: According to ANOVA, significant demineralization variations (ΔD) were determined among groups (F=6.650; P<.01). The ACP-containing composite showed the lowest (mean: 8.98±2.38) and the control composite showed the highest (mean:12.15±3.83) ΔD, during 21 days demineralization process (P<.01). Significant difference was also observed between the ΔD scores of the RMGIC (mean: 9.24±2.73) and control (P<.05).No significant differences was found in preventive effects of ACP-containing composite and RMGIC (P<.05) against demineralization.Conclusions: The use of both ACP-containing orthodontic composite and RMGIC should be recommended for any at-risk orthodontic patient to provide preventive actions and potentially remineralize subclinical enamel demineralization. (Eur J Dent 2009;3:127-134)

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