scholarly journals The effects of fluoride on normal human diploid cells. Studies on their growth, protein synthesis, ALP activity, cAMP levels and ultrastructure.

1987 ◽  
Vol 37 (2) ◽  
pp. 172-184
Author(s):  
Tsutomu SATO ◽  
Motoo NIWA
Keyword(s):  
Protein Synthesis ◽  
Alp Activity ◽  
Normal Human ◽  
Human Diploid Cells ◽  
Diploid Cells ◽  
Camp Levels

scholarly journals Nuclear accumulation of cyclin D1 following long-term fractionated exposures to low-dose ionizing radiation in normal human diploid cells

Cell Cycle ◽  
2014 ◽  
Vol 13 (8) ◽  
pp. 1248-1255 ◽  
Author(s):  
Tsutomu Shimura ◽  
Nobuyuki Hamada ◽  
Megumi Sasatani ◽  
Kenji Kamiya ◽  
Naoki Kunugita
Keyword(s):  
Ionizing Radiation ◽  
Cyclin D1 ◽  
Low Dose ◽  
Nuclear Accumulation ◽  
Normal Human ◽  
Human Diploid Cells ◽  
Diploid Cells

scholarly journals Evidence for endogenous polypeptide-mediated inhibition of cell-cycle transit in human diploid cells.

1982 ◽  
Vol 94 (1) ◽  
pp. 187-192 ◽  
Author(s):  
G C Burmer ◽  
C J Zeigler ◽  
T H Norwood
Keyword(s):  
Cell Cycle ◽  
Protein Synthesis ◽  
Dna Synthesis ◽  
Tritiated Thymidine ◽  
Protein Synthesis Inhibitor ◽  
Synthesis Inhibitor ◽  
Human Diploid Fibroblasts ◽  
Senescent Phenotype ◽  
Human Diploid Cells ◽  
Diploid Cells

Previous studies have shown that the senescent phenotype is dominant with respect to DNA synthesis in fusions between late passage and actively replicating human diploid fibroblasts. Brief postfusion treatments with the protein synthesis inhibitor cycloheximide (CHX) or puromycin have been found to significantly delay (by 24-48 h) the inhibition of entry into DNA synthesis of young nuclei in heterokaryons after fusion with senescent cells. A significant fraction of the senescent nuclei incorporated tritiated thymidine in CHX-treated heterokaryons. The optimal duration of exposure to CHX was 1-3 h immediately after fusion, although treatments beginning as late as 9 h after fusion elevated the heterokaryon labeling index. Prefusion treatments with CHX were without a significant effect. These results are consistent with the interpretation that regulatory cell cycle inhibitor(s) which are dependent upon protein synthesis may be present in heterokaryons between senescent and actively replicating cells.

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