Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species

2016 ◽  
Vol 6 (1) ◽  
pp. 54-65 ◽  
Author(s):  
Michaela Marečková ◽  
Miloš Barták
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Temperature Effects ◽  
Photochemical Quenching ◽  
Short Term ◽  
Ps Ii ◽  
Species Specific ◽  
Non Photochemical Quenching ◽  
Fluorescence Transients ◽  
Chlorophyll Fluorescence Transients

Chlorophyll fluorescence is an effective tool for investigating characteristics of any photosynthesizing organisms and its responses due to different stressors. Here, we have studied a short-term temperature response on two Antarctic green algal lichen species: Umbilicaria antarctica, and Physconia muscigena. We measured slow chlorophyll fluorescence transients in the species during slow a cooling of thallus temperature from 20°C to 5°C with a 10 min. acclimation at each temperature in dark. The measurements were supplemented with saturation pulses for the analysis of chlorophyll fluorescence parameters: maximum yield of PS II photochemistry (FV/FM), effective quantum yield of PS II photochemistry (FPSII) and non-photochemical quenching (NPQ). In response to decreasing thallus temperature, we observed species-specific changes in chlorophyll fluorescence levels P, S, M, T reached during chlorophyll fluorescence transient as well as in the shape of the chlorophyll fluorescence transients. With a decrease in temperature, the time at which M and T chlorophyll fluorescence levels were reached, increased. These changes were attributed to redox state of plastoquinon pool, changes in Calvin-Benson cycle activity, non-photochemical quenching components, state transition in particular. In this study, we present some chlorophyll fluorescence ratios (P/M, M/T, P/T) and chlorophyll fluorescence increase rates (FR1, i.e. O to P, and FR2 - i.e. S to M) as the parameters reflecting direct temperature effects on chloroplastic apparatus of lichen alga sensitively. We proposed that species-specific changes in the slow phase of chlorophyll fluorescence transients could be potentially used as indicators of low temperature effects in photosynthetic apparatus of lichen algal photobionts. Interspecific differences in response to low temperature might be evaluated using the approach as well.

Features of chlorophyll fluorescence transients can be used to investigate low temperature induced effects on photosystem II of algal lichens from polar regions ( Short Communication )

2015 ◽  
Vol 5 (1) ◽  
pp. 99-111 ◽  
Author(s):  
Anamika Mishra ◽  
Josef Hájek ◽  
Tereza Tuháčková ◽  
Miloš Barták ◽  
Kumud Bandhu Mishra
Keyword(s):  
Chlorophyll Fluorescence ◽  
Lichen Species ◽  
Freezing Stress ◽  
Slow Phase ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Species Specific ◽  
Fluorescence Transients ◽  
Chlorophyll Fluorescence Transients

Chlorophyll fluorescence is an effective tool for investigating characteristics of any photosynthesizing organisms and its responses due to different stressors. Here, we have studied a short-term temperature response on three Antarctic green algal lichen species: Umbilicaria antarctica, Xanthoria elegans, and Rhizoplaca melanophtalma. We measured slow chlorophyll fluorescence transients in these Antarctic lichen species during slowely cooling of thallus temperature from 20°C to 5, 0 and -5°C with 20 minute acclimation at each temperature. The measurements were supplemented with saturation pulses for the analysis of chlorophyll fluorescence parameters: maximum yield of PS II photochemistry (FV/FM), effective quantum yield of PS II photochemistry (FPSII) and quenching parameters. In response to decreasing thallus temperature, we observed species-specific changes in chlorophyll fluorescence parameters as well as in the shape of the chlorophyll fluorescence transients. We propose that species-specific changes in the slow phase of chlorophyll fluorescence transients can be potentially used as indicators of freezing stress in photosynthetic apparatus of lichen algal photobionts.

UV-B effects on filamentous alga Zygnema strain (EEL201) from Antarctica

2016 ◽  
Vol 6 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Marina Prieto-Amador
Keyword(s):  
Chlorophyll Fluorescence ◽  
Summer Season ◽  
Filamentous Alga ◽  
Stress Factors ◽  
Photochemical Quenching ◽  
Negative Effects ◽  
Short Term ◽  
Ps Ii ◽  
High Irradiation ◽  
Freshwater Availability

Filamentous alga Zygnema sp. is frequently found in extreme polar environments with freshwater availability for at least part of summer season. In such habitats, Zygnema might be exposed to several stress factors, like freeze, desiccation and high irradiation levels. This study investigated the effect of UV-B on primary photosynthetic processes in Zygnema sp. (EEL201 strain) from Antarctica. Samples were cultivated in liquid medium and exposed to supplemental UV-B (1.4 W m-2) for 6 h. During the UV-B treatment and following recovery, the changes in chlorophyll fluorescence paramaters caused UV-B were measured. Negative effects on FV/FM and FPSII were found after 6 h treatment with only limited recovery in dark. The only parameter that recovered was photochemical quenching (qP) indicating a potential to restore photosynthesis in the reaction centres that were not damaged by UV-B treatment. However, the share on damaged RC PS II was much higher compared to those showing the recovery. Thus, the effect of short-term supplemental 1.4 W m-2 UV-B light was considered heavy causing substantial damages to PS II. These results provide insights on the effects of UV-B light onZygnemasp. that can help in the interpretation of response mechanisms of Arctic algae to radiation.

Trapping of the quenched conformation associated with non-photochemical quenching of chlorophyll fluorescence at low temperature

2007 ◽  
Vol 94 (2-3) ◽  
pp. 321-332 ◽  
Author(s):  
Petar H. Lambrev ◽  
Tsonko Tsonev ◽  
Violeta Velikova ◽  
Katya Georgieva ◽  
Maya D. Lambreva ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Photochemical Quenching ◽  
Non Photochemical Quenching

scholarly journals Photosynthesis sensitivity to NH4+-N change with nitrogen fertilizer type

2014 ◽  
Vol 60 (No. 6) ◽  
pp. 274-279 ◽  
Author(s):  
A. Nasraoui-Hajaji ◽  
H. Gouia
Keyword(s):  
Net Photosynthesis ◽  
Dry Weight ◽  
N Fertilization ◽  
Photochemical Quenching ◽  
Negative Effects ◽  
Ps Ii ◽  
Chl A ◽  
Inhibitory Site ◽  
Tomato Growth ◽  
Non Photochemical Quenching

N-fertilization type affected differently tomato growth. In the field experiment, hydroponic cultures were conducted using NO<sub>3</sub>-N (5 mmol); mixture of KNO<sub>3</sub>-N (3 mmol) and (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>-N (2 mmol); NH<sub>4</sub><sup>+</sup>-N (5 mmol) or urea&nbsp;(5 mmol) as nitrogen source. Compared to nitrate, ammonium and urea had negative effects on morphology and dry matter production. Effects of the different nitrogen forms were investigated by measuring several photosynthesis parameters and chl a fluorescence. Two different significant types of reaction were found. When nitrogen was added as ammonium or urea, dry weight, chlorophyll tenor, transpiration rate, stomatal conductance and photosynthetic activity were inhibited. Supply of ammonium or urea, reduced the ratio (F<sub>v</sub>/F<sub>m</sub>), photochemical quenching and enhanced the non photochemical quenching. These data suggest that the adverse decrease in tomato growth under ammonium or urea supply may be related principally to inhibition of net photosynthesis activity. The high non photochemical quenching shown in tomato fed with ammonium or urea indicated that PS II was the inhibitory site of NH<sub>4</sub><sup>+</sup>-N which was directly uptaken by roots, or librated via urea hydrolysis cycle.

Salt stress resilience potential of a fungal inoculant isolated from tea cultivation area in maize

Biologia ◽  
2017 ◽  
Vol 72 (6) ◽  
Author(s):  
Nuran Durmus ◽  
Abdullah Muhammed Yesilyurt ◽  
Necla Pehlivan ◽  
Sengul Alpay Karaoglu
Keyword(s):  
Salt Stress ◽  
Water Status ◽  
Cost Effective ◽  
Nacl Stress ◽  
Photochemical Quenching ◽  
Effective Quantum Yield ◽  
Stress Resilience ◽  
Ps Ii ◽  
Tea Plants ◽  
Non Photochemical Quenching

AbstractAgriculture needs to be sustained by organic processes in current era as population explosion energy and the number of individuals undernourished are raising public concerns. Global warming poses additional threat by lifting the damage of salt stress especially in agro-economically vital crops like maize whose cultivation dates back to Mayans. To that end, cost-effective and organic fungal agents may be great candidates in stress resilience. We isolated the fungal strain from the soil of tea plants and characterized that via 5.8 S rDNA gene with internal transcribed spacer ITS-1 and ITS-2 regions, then named the target strain as TA. Reduced maximum quantum efficiency of PS II (Fv/Fm), the effective quantum yield of PS2 (ΦPS2), electron transport rate (ETR), photochemical quenching (qP) and increased non-photochemical quenching (NPQ) were detected in maize plants stressed with dose dependent salt. Enhanced Fv/Fm, ΦPS2, ETR, qP and decreased NPQ was observed in TA primed plus NaCl treated plants. TA biopriming significantly increased the lengths, fresh and dry weights of root/shoots and decreased the lipid peroxidation. Maize seedlings bioprimed with TA had less MDA and higher soluble protein, proline, total chlorophyll, carotenoid and RWC under NaCl. Furthermore, SOD, GPX and GR activities were much more increased in root and leaves of TA primed seedlings, however CAT activity did not significantly change. This is the first report to our knowledge that TA reverses the damage of NaCl stress on maize growth through improving water status, antioxidant machinery and especially photosynthetic capacity.

Temperature response of dry matter accumulation, leaf photosynthesis, and chlorophyll fluorescence in an old and a new maize hybrid during early development

1991 ◽  
Vol 71 (2) ◽  
pp. 353-359 ◽  
Author(s):  
M. Tollenaar ◽  
M. Mihajlovic ◽  
A. Aguilera
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Dry Matter ◽  
Temperature Tolerance ◽  
Dry Matter Accumulation ◽  
Leaf Photosynthesis ◽  
Short Term ◽  
Dry Matter Partitioning ◽  
Leaf Stage ◽  
Low Temperature Tolerance

Studies were conducted to investigate whether genetic improvement in dry matter accumulation of maize (Zea mays L.) hybrids recommended in Ontario from the late 1950s to the late 1980s is associated with cold-temperature tolerance during early phases of development. The maize hybrids Pride 5 (released in 1959) and Pioneer 3902 (released in 1988) were compared at 16/7, 23/14, and 33/24 °C under a 16-h photoperiod with a photosynthetic photon flux density of 650 μmol m−2 s−1 in long-term and short-term temperature experiments conducted in controlled-environment cabinets. In the long-term temperature experiment, plants were grown at the three temperature regimes from the 4- to the 12-leaf stage. Total and plant component dry matter was determined at the 8-, 10-, and 12-leaf stage, and leaf photosynthesis and chlorophyll fluorescence were measured at the 10-leaf stage. In the short-term temperature experiment, plants were exposed to the three temperature regimes during a 3-d period after the 9-leaf stage, followed by 2 d at 23/14 °C. Dry matter accumulation during the 5-d period was measured and leaf photosynthesis and chlorophyll fluorescence were measured during each of the last 3 d of the 5-d period. Results showed a highly significant temperature effect on all measured parameters. Dry matter of Pride 5 at the 10-leaf stage was higher than that of Pioneer 3902, but rates of dry matter accumulation and leaf photosynthesis did not differ among hybrids, and hybrid × temperature interactions were not significant for these parameters. Hybrid × temperature interactions were significant for dry matter partitioning and the fluorescence parameter Fv/Fm, suggesting better low-temperature tolerance for Pride 5. Results of the short-term temperature study showed a significant hybrid × temperature interaction for dry matter accumulation, with Pride 5 higher than Pioneer 3902 at the low temperature regime and Pride 5 lower than Pioneer 3902 at the high temperature regime. Results of these experiments suggest that improvement over the past 30 yr of Ontario maize hybrids is not associated with improved low-temperature tolerance during early development. Key words: Maize, low-temperature tolerance, dry matter accumulation, dry matter partitioning, photosynthesis, chlorophyll fluorescence

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