Transfusion-Transmitted Cytomegalovirus Infection (TT-CMV): A Pilot Study on Safety of Whole-Blood Derivatives

Scimetr ◽  
2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Mishar Kelishadi ◽  
Abdolvahab Moradi ◽  
Sobhan Samadi ◽  
Pezhman Hashemi ◽  
Masoud Bazouri ◽  
...  
Keyword(s):  
Pilot Study ◽  
Whole Blood ◽  
Cytomegalovirus Infection

Pilot Study of Whole Blood MicroRNAs as Potential Tools for Diffuse Low-Grade Gliomas Detection

2017 ◽  
Vol 38 (3) ◽  
pp. 715-725 ◽  
Author(s):  
Catherine Gozé ◽  
Christelle Reynes ◽  
Lionel Forestier ◽  
Robert Sabatier ◽  
Hugues Duffau
Keyword(s):  
Pilot Study ◽  
Whole Blood ◽  
Low Grade ◽  
Low Grade Gliomas

scholarly journals Circulating Ionized Magnesium as a Measure of Supplement Bioavailability: Results from a Pilot Study for Randomized Clinical Trial

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1245 ◽  
Author(s):  
Jiada Zhan ◽  
Taylor C. Wallace ◽  
Sarah J. Butts ◽  
Sisi Cao ◽  
Velarie Ansu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Whole Blood ◽  
Area Under The Curve ◽  
Oral Intake ◽  
Magnesium Content ◽  
Magnesium Concentration ◽  
Rank Test ◽  
Ionized Magnesium ◽  
Signed Rank Test ◽  
Total Magnesium

Oral supplementation may improve the dietary intake of magnesium, which has been identified as a shortfall nutrient. We conducted a pilot study to evaluate appropriate methods for assessing responses to the ingestion of oral magnesium supplements, including ionized magnesium in whole blood (iMg2+) concentration, serum total magnesium concentration, and total urinary magnesium content. In a single-blinded crossover study, 17 healthy adults were randomly assigned to consume 300 mg of magnesium from MgCl2 (ReMag®, a picosized magnesium formulation) or placebo, while having a low-magnesium breakfast. Blood and urine samples were obtained for the measurement of iMg2+, serum total magnesium, and total urine magnesium, during 24 h following the magnesium supplement or placebo dosing. Bioavailability was assessed using area-under-the-curve (AUC) as well as maximum (Cmax) and time-to-maximum (Tmax) concentration. Depending on normality, data were expressed as the mean ± standard deviation or median (range), and differences between responses to MgCl2 or placebo were measured using the paired t-test or Wilcoxon signed-rank test. Following MgCl2 administration versus placebo administration, we observed significantly greater increases in iMg2+ concentrations (AUC = 1.51 ± 0.96 vs. 0.84 ± 0.82 mg/dL•24h; Cmax = 1.38 ± 0.13 vs. 1.32 ± 0.07 mg/dL, respectively; both p < 0.05) but not in serum total magnesium (AUC = 27.00 [0, 172.93] vs. 14.55 [0, 91.18] mg/dL•24h; Cmax = 2.38 [1.97, 4.01] vs. 2.24 [1.98, 4.31] mg/dL) or in urinary magnesium (AUC = 201.74 ± 161.63 vs. 139.30 ± 92.84 mg•24h; Cmax = 26.12 [12.91, 88.63] vs. 24.38 [13.51, 81.51] mg/dL; p > 0.05). Whole blood iMg2+ may be a more sensitive measure of acute oral intake of magnesium compared to serum and urinary magnesium and may be preferred for assessing supplement bioavailability.

A multicenter comparison of whole blood vitamin B6 assays

2016 ◽  
Vol 54 (4) ◽  
Author(s):  
Bertrand D. van Zelst ◽  
Roseri J.A.C. Roelofsen de Beer ◽  
Marjolein Neele ◽  
Snježana Kos ◽  
Ido P. Kema ◽  
...  
Keyword(s):  
Pilot Study ◽  
Whole Blood ◽  
Analytical Methods ◽  
Vitamin B6 ◽  
Method Comparison ◽  
Reference Method ◽  
Hplc Method ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Suitable Reference

AbstractThe aim of this study was to compare different analytical methods that are currently in use in the Netherlands for the measurement of whole blood vitamin B6.This method comparison study consisted of two separate parts. (1) Four laboratories participated in a pilot study in which the commercial Chromsystems and INstruchemie method, and a laboratory developed liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and HPLC method were compared. Sixty-nine frozen whole blood samples and six lyophilized whole blood samples were used for comparison. (2) In the nationwide part of the study, 49 laboratories participated in the analysis of three identical sets of two whole blood samples of which one set was freshly analyzed, one set was analyzed after storage at −20 °C and one set was analyzed after lyophilization.In both parts of the study, the HPLC and LC-MS/MS methods showed equivalent results for all sample types tested. The Chromsystems method showed a positive bias of 45% (pilot study) and 30% (nationwide study) towards the LC-MS/MS method when fresh or frozen samples were used. The measurement of lyophilized samples showed no differences between the methods. The results of the INstruchemie method were inconclusive due to the low number of participants.The different analytical methods for measuring vitamin B6 produce different results when whole blood patient samples are measured. The recognition of a reference method or the development of suitable reference materials and quality control materials might serve as a first step towards improved standardization or harmonization of the whole blood vitamin B6 assay.

scholarly journals The Prevalence of Human Cytomegalovirus Infection in Children Leukemia by Chip Digital PCR

2021 ◽  
Author(s):  
Wen-Jun Wang ◽  
Miao Feng ◽  
Feng He ◽  
Juan Song ◽  
Qin-Qin Song ◽  
...  
Keyword(s):  
Viral Load ◽  
Human Cytomegalovirus ◽  
Whole Blood ◽  
Cytomegalovirus Infection ◽  
Hcmv Infection ◽  
Laboratory Diagnosis ◽  
Digital Pcr ◽  
Clinical Treatment ◽  
Diagnosis Rate ◽  
Human Cytomegalovirus Infection

Abstract Background To establish a method for detecting HCMV viral load to guide clinical treatment by chip digital PCR (cdPCR). Methods 5.67×106TCID50/ml of HCMV AD169 was serially diluted to evaluate sensitive of cdPCR, HSV-1, HSV-2, VZV, EBV, HHV-6 and HHV-7 were used to evaluate the specificity of HCMV cdPCR. HCMV infection were analyzed in 110 children leukemia whole blood by RT-qPCR and cdPCR. Results The sensitive of HCMV cdPCR was up to 71 ± 32 copies/ml, which is higher than that of RT-qPCR. HCMV cdPCR did not cross react with other herpesviruses. The cdPCR effectively detected 7 HCMV positive samples, making the laboratory diagnosis rate of HCMV increased by 6.36% (7/110) for children leukemia patients. And the prevalence of HCMV infection increased from 28.18–34.54% in 110 children leukemia patients by cdPCR. Conclusion cdPCR is more sensitive to detect viral load than RT-qPCR. The cdPCR may be used to evaluate relationship between viral load and progression of HCMV infection in patients.

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