Molecular Detection of Macrolide and Lincosamide-Resistance Genes in Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Kerman, Iran

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Yasser Fasihi ◽  
Fereshteh Saffari ◽  
Mohammad Reza Kandehkar Ghahraman ◽  
Davood Kalantar-Neyestanaki
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Genes ◽  
Molecular Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

Molecular Detection of Antibiotic and Antiseptic Resistance Genes Among Methicillin Resistant Staphylococcus aureus

2015 ◽  
Vol 43 (2) ◽  
pp. 13-23
Author(s):  
Ahmed Ammar ◽  
Adel Attia ◽  
Ibrahim El-Shorbagy ◽  
Marwa Abd El-Hamid ◽  
Shaimaa Abd El-Kader
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Genes ◽  
Molecular Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

scholarly journals The First Report of a Methicillin-Resistant Staphylococcus aureus Isolate Harboring Type IV SCCmec in Thailand

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 430
Author(s):  
Wichai Santimaleeworagun ◽  
Praewdow Preechachuawong ◽  
Wandee Samret ◽  
Tossawan Jitwasinkul
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Sccmec Type ◽  
Clinical Strain ◽  
Spa Typing ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Antimicrobial Resistance Genes

Methicillin-resistant Staphylococcus aureus (MRSA) is mostly found in Thailand in the hospital as a nosocomial pathogen. This study aimed to report the genetic characterization of a clinical community-acquired MRSA (CA-MRSA) isolate collected from hospitalized patients in Thailand. Among 26 MRSA isolates, S. aureus no. S17 preliminarily displayed the presence of a staphylococcal cassette chromosome mec (SCCmec) type IV pattern. The bacterial genomic DNA was subjected to whole-genome sequencing. Panton–Valentine leukocidin (PVL) production, virulence toxins, and antibiotic resistance genes were identified, and multi-locus sequence typing (MLST) and spa typing were performed. The strain was matched by sequence to MLST type 2885 and spa type t13880. This strain carried type IV SCCmec with no PVL production. Five acquired antimicrobial resistance genes, namely blaZ, mecA, Inu(A), tet(K), and dfrG conferring resistance to β-lactams, lincosamides, tetracycline, and trimethoprim, were identified. The detected toxins were exfoliative toxin A, gamma-hemolysin, leukocidin D, and leukocidin E. Moreover, there were differences in seven regions in CR-MRSA no. S17 compared to CA-MRSA type 300. In summary, we have reported the ST2885-SCCmec IV CA-MRSA clinical strain in Thailand for the first time, highlighting the problem of methicillin resistance in community settings and the consideration in choosing appropriate antibiotic therapy.

scholarly journals Genome Sequence of a Recently Emerged, Highly Transmissible, Multi-Antibiotic- and Antiseptic-Resistant Variant of Methicillin-Resistant Staphylococcus aureus, Sequence Type 239 (TW)

2009 ◽  
Vol 192 (3) ◽  
pp. 888-892 ◽  
Author(s):  
Matthew T. G. Holden ◽  
Jodi A. Lindsay ◽  
Craig Corton ◽  
Michael A. Quail ◽  
Joshua D. Cockfield ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Housekeeping Genes ◽  
Sequence Type ◽  
Resistant Variant ◽  
Methicillin Resistant ◽  
Wide Range

ABSTRACT The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile genetic elements and also mutations in housekeeping genes.

scholarly journals Molecular Detection of Methicillin-Resistant Staphylococcus aureus by Non-Protein Coding RNA-Mediated Monoplex Polymerase Chain Reaction

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158736 ◽  
Author(s):  
Cheryl Yeap Soo Yean ◽  
Kishanraj Selva Raju ◽  
Rathinam Xavier ◽  
Sreeramanan Subramaniam ◽  
Subash C. B. Gopinath ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Molecular Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Chain Reaction ◽  
Protein Coding ◽  
Methicillin Resistant ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document