scholarly journals Characterization of Uropathogenic Escherichia coli: Distribution of Adhesin-Encoding Genes and O-Serotypes Among Ciprofloxacin Susceptible and Resistant Isolates

2019 ◽  
Vol 12 (9) ◽  
Author(s):  
Ahmad Rashki ◽  
Masuod Rahdar ◽  
Zahra Rashki Ghalehnoo
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Encoding Genes

scholarly journals Characterization of auto-agglutinating and non-typeable uropathogenic Escherichia coli strains

2019 ◽  
Vol 13 (06) ◽  
pp. 465-472
Author(s):  
Ulises Hernández-Chiñas ◽  
Alejandro Pérez-Ramos ◽  
Laura Belmont-Monroy ◽  
María E Chávez-Berrocal ◽  
Edgar González-Villalobos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Resistant Strains ◽  
Tract Infections ◽  
Disk Method

Introduction: Uropathogenic Escherichia coli (UPEC) are the main etiological agent of urinary tract infections (UTIs). Association between different serotypes and UTIs is known, however, some strains are incapable to be serotyped. The aim of this work was to study bthe phenotypical and genotypical characteristics of 113 non-typeable (NT) and auto-agglutinating (AA) E. coli strains, isolated from UTIs in children and adults. Methodology: The 113 UPEC strains were analyzed by PCR assays using specific primers to determine their serogroups, fimH, papC, iutA, sat, hlyCA and cnf1, virulence associated genes, and chuA, yjaA and TSPE4.C2 for phylogroup determination. Additionally, the diffusion disk method was performed to evaluate the antimicrobial resistance to 18 antimicrobial agents. Results: Using the PCR assay, 63% (71) of the strains were genotyped showing O25 and O75 as the most common serogroups. The virulence genes fimH (86%) and iutA (74%) were the most prevalent, in relation to the phylogroups the commensal (A and B1) and virulent (B2 and D) showed similar frequencies (P > 0.05). The antimicrobial susceptibility test showed a high percentage (73%) of multidrug-resistant strains. Conclusions: The genotyping allowed identifying the serogroup in many of the strains that could not be typed by traditional serology. The strains carried virulence genes and were multidrug-resistant in both, commensal and virulent phylogroups. Our findings revealed that, in addition to the classical UPEC serogroups, there are pathogenic serogroups not reported yet.

scholarly journals Molecular Characterization of Uropathogenic Escherichia coli Reveals Emergence of Drug Resistant O15, O22 and O25 Serogroups

Medicina ◽  
2019 ◽  
Vol 55 (11) ◽  
pp. 733 ◽  
Author(s):  
Ruta Prakapaite ◽  
Frederic Saab ◽  
Rita Planciuniene ◽  
Vidmantas Petraitis ◽  
Thomas J. Walsh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Asymptomatic Bacteriuria ◽  
Empirical Therapy ◽  
Uropathogenic Escherichia Coli ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Tract Infections ◽  
Resistance Rates

Background and Objectives: Uropathogenic Escherichia coli (UPEC) are common pathogens causing urinary tract infections (UTIs). We aimed to investigate the relationship among clinical manifestation, serogroups, phylogenetic groups, and antimicrobial resistance among UPEC. Materials and Methods: One-hundred Escherichia coli isolates recovered from urine and ureteral scrapings were used for the study. The prevalence of antimicrobial resistance was determined by using European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommendations. E. coli serogroups associated with UTI, as well as phylogenetic diversity were analyzed using multiplex PCR reactions. Results: Eighty-seven strains (87%) were isolated from females, while 13 (13%) from males. A high frequency of resistance to cephalosporins (43%) and fluoroquinolones (31%) was observed. Among UTI-associated serogroups O15 (32.8%), O22 (23.4%), and O25 (15.6%) were dominant and demonstrated elevated resistance rates. The E. coli phylogenetic group B2 was most common. These observations extended to pregnant patients with asymptomatic bacteriuria. Conclusions: Due to high rates of resistance, strategies using empirical therapy of second-generation cephalosporins and fluoroquinolones should be reconsidered in this population.

Identification and characterization of class 1 integrons among multidrug-resistant uropathogenic Escherichia coli strains in Mexico

2022 ◽  
Vol 162 ◽  
pp. 105348
Author(s):  
Edgar González-Villalobos ◽  
Rosa María Ribas-Aparicio ◽  
Laura Belmont-Monroy ◽  
Gerardo Aparicio-Ozores ◽  
H. Ángel Manjarrez-Hernández ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Identification And Characterization

scholarly journals Genomic characterization of NDM-1 and 5, and OXA-181 carbapenemases in uropathogenic Escherichia coli isolates from Riyadh, Saudi Arabia

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0201613 ◽  
Author(s):  
Moataz Abd El Ghany ◽  
Hazem Sharaf ◽  
Mohamed H. Al-agamy ◽  
Atef Shibl ◽  
Grant A. Hill-Cawthorne ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
Uropathogenic Escherichia Coli ◽  
Genomic Characterization
Sign in / Sign up

Export Citation Format

Share Document