scholarly journals Microsatellite Length Polymorphism for DNA-Based Typing of Candida albicans Isolated from HIV Positive Patients in Tehran, Iran

2018 ◽  
Vol 11 (5) ◽  
Author(s):  
Ehsan Farahbakhsh ◽  
Farzad Katiraee ◽  
Shahla Roudbar Mohammadi ◽  
Shirin Shahbazi ◽  
Mohammad Hossein Yadegari
Keyword(s):  
Candida Albicans ◽  
Length Polymorphism ◽  
Hiv Positive ◽  
Microsatellite Length Polymorphism

scholarly journals Comparison of Microsatellite Length Polymorphism and Multilocus Sequence Typing for DNA-Based Typing of Candida albicans

2007 ◽  
Vol 45 (12) ◽  
pp. 3958-3963 ◽  
Author(s):  
D. Garcia-Hermoso ◽  
O. Cabaret ◽  
G. Lecellier ◽  
M. Desnos-Ollivier ◽  
D. Hoinard ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Length Polymorphism ◽  
Multilocus Sequence Typing ◽  
Microsatellite Length Polymorphism

Molecular epidemiology of Candida albicans strains isolated from the oropharynx of HIV-positive patients at successive clinic visits

2001 ◽  
Vol 39 (4) ◽  
pp. 341-352 ◽  
Author(s):  
B. A. Lasker ◽  
C. M. Elie ◽  
T. J. Lott ◽  
A. Espinel-Ingroff ◽  
L. Gallagher ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Molecular Epidemiology ◽  
Hiv Positive ◽  
Clinic Visits

scholarly journals Comparison of MultiLocus Sequence Typing (MLST) and Microsatellite Length Polymorphism (MLP) for Pneumocystis jirovecii genotyping

2020 ◽  
Vol 18 ◽  
pp. 2890-2896
Author(s):  
Maud Gits-Muselli ◽  
Pascal Campagne ◽  
Marie Desnos-Ollivier ◽  
Patrice Le Pape ◽  
Stéphane Bretagne ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Multilocus Sequence Typing ◽  
Pneumocystis Jirovecii ◽  
Microsatellite Length Polymorphism

scholarly journals Evaluation of virulence factors of Candida albicans isolated from HIV-positive individuals using HAART

2016 ◽  
Vol 66 ◽  
pp. 61-65 ◽  
Author(s):  
Ralciane de Paula Menezes ◽  
Érika Bezerra de Melo Riceto ◽  
Aércio Sebastião Borges ◽  
Denise Von Dolingër de Brito Röder ◽  
Reginaldo dos Santos Pedroso
Keyword(s):  
Candida Albicans ◽  
Virulence Factors ◽  
Hiv Positive

scholarly journals Genetic Analysis of Candida glabrata from Candiduric Patients Using Microsatellite Length Polymorphism, Antifungal Susceptibility, and Enzymatic Profiles

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Maral Gharaghani ◽  
Ali Rezaei-Matehkolaei ◽  
Amir Kamal Hardani ◽  
Ali Zarei Mahmoudabadi
Keyword(s):  
Enzymatic Activity ◽  
Microsatellite Markers ◽  
Length Polymorphism ◽  
Spanning Tree ◽  
Candida Glabrata ◽  
Minimum Spanning Tree ◽  
Antifungal Susceptibility ◽  
Wild Type ◽  
High Genetic Diversity ◽  
Microsatellite Length Polymorphism

Background: Candida glabrata is the second agent of candiduria with increased resistance to antifungals. Microsatellite length polymorphism (MLP) is one of the genotyping techniques used in the epidemiological investigation to improve clinical management. Objectives: We aimed to detect different genotypes of C. glabrata isolates using six microsatellite markers and the MLP technique. Moreover, our genotypes' association with other countries' genotypes was illustrated using a minimum spanning tree. We investigated in vitro antifungal susceptibility and enzymatic activity profiles of the isolates. Methods: Six microsatellite markers were amplified using multiplex-PCR for 22 C. glabrata strains isolated from urine in pediatric patients admitted to the Abuzar Children's Hospital in Ahvaz, Iran. The PCR products were presented for fragment analysis, and the size of the alleles was determined. Antifungal susceptibility tests and extracellular enzyme activities were also performed. Results: Nineteen multilocus genotypes were detected so that 22.7% of the strains had identical genotypes. The isolates were wild-type for amphotericin B (0.0625 - 2 µg/mL), itraconazole (0.125 - 2 µg/mL), and voriconazole (0.0078 - 0.00625 µg/mL). All the isolates were sensitive to fluconazole at the minimum inhibitory concentration (MIC) range (0.0312 - 16 μg/mL), and three of them were resistant to caspofungin (MIC ≥ 0.5 μg/mL). Moreover, 72.7 and 68.2% of the isolates had no phospholipase and esterase activities. The highest potency of enzymatic activity was obtained in hemolysin and proteinase enzymes. A high genetic diversity (19 genotypes of the 22 isolates) existed among the urinary C. glabrata isolates. Based on the minimum spanning tree, two clusters of our genotypes were related to C. glabrata genotypes in a previous study in Iran, and the third cluster was entirely connected with Chinese genotypes. Conclusions: Most of the isolates were the non-wild type for posaconazole but were rarely resistant to other antifungals. Hemolysin and proteinase secreted as the main virulence factors among the urinary C. glabrata isolates.

Ectophosphatase activity in Candida albicans influences fungal adhesion: study between HIV-positive and HIV-negative isolates

Oral Diseases ◽  
2010 ◽  
Vol 16 (5) ◽  
pp. 431-437 ◽  
Author(s):  
MB Portela ◽  
LF Kneipp ◽  
IP Ribeiro de Souza ◽  
C Holandino ◽  
CS Alviano ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Hiv Positive ◽  
Fungal Adhesion ◽  
Hiv Negative
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