scholarly journals A Study on the Virus-Like Particle Formation of Hepatitis E Virus ORF2 and Rotavirus NSP4 Protein in the Eukaryotic and Prokaryotic Expression Systems

2017 ◽  
Vol 10 (12) ◽  
Author(s):  
Manoochehr Makvandi ◽  
Ali Teimoori ◽  
Niloofar Neisi ◽  
Alireza Samarbafzadeh
Keyword(s):  
Prokaryotic Expression ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Particle Formation ◽  
Expression Systems ◽  
Virus Like Particle ◽  
Nsp4 Protein

scholarly journals Role of the C-terminal cysteines in virus-like particle formation and oligomerization of the hepatitis E virus ORF2 truncated proteins

Virology ◽  
2020 ◽  
Vol 544 ◽  
pp. 1-11 ◽  
Author(s):  
Zhenzhen Liu ◽  
Nouredine Behloul ◽  
Sarra Baha ◽  
Wenjuan Wei ◽  
Wanru Tao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Particle Formation ◽  
Virus Like Particle

scholarly journals Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle

2008 ◽  
Vol 64 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Che-Yen Wang ◽  
Naoyuki Miyazaki ◽  
Tetsuo Yamashita ◽  
Akifumi Higashiura ◽  
Atsushi Nakagawa ◽  
...  
Keyword(s):  
Diffraction Analysis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
X Ray Diffraction ◽  
X Ray ◽  
Virus Like Particle

scholarly journals Hepatitis E virus truncated capsid protein as a potential carrier of exogenous antigens for the development of chimeric virus-like particle vaccines

2019 ◽  
Author(s):  
Tianyu LU ◽  
Nouredine BEHLOUL ◽  
Yi ZHOU ◽  
Sarra BAHA ◽  
Zhenzhen LIU ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Foot And Mouth Disease ◽  
Vaccine Design ◽  
Hepatitis E ◽  
Mouth Disease ◽  
Virus Like Particle ◽  
Orf2 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Abstract Background: Virus like particle (VLP), a multiprotein structure which is assembled automatically, can stimulate robust immune responses due to an appropriate size, repetitive epitopes, and a structure similar to native virions. Utilizing VLPs as vaccine carriers to present exogenous antigens is a promising and challenging field in vaccine design. Hence, this study aims to investigate the potential of Hepatitis E virus (HEV) truncated capsid protein as a VLP carrier presenting foreign antigens in vaccine design.Results: S and M domains of HEV ORF2 protein (aa112-455) were selected as an optimal carrier (CaSM). The exogenous antigen Seq8 containing three immunogenic domains from three different foot-and-mouth disease virus (FMDV) strains was linked to the C-terminal of CaSM to construct a chimeric VLP vaccine candidate (CaSM-Seq8). Morphological analysis showed that CaSM-Seq8 self-assembled into VLPs with a diameter of approximately 26 nm, similar to the VLPs of CaSM alone but smaller than native HEV virions. Further, the thermal stability and the proteolysis resistance of Seq8 were enhanced when carried by CaSM. The antigenicity analysis revealed a more robust reactivity against anti-FMDV specific antibodies when Seq8 was presented on the CaSM particles. Upon injection into mice, anti-FMDV IgGs induced by CaSM-Seq8 appeared earlier, increased faster, and maintained higher levels for a longer time than those induced by Seq8 antigen alone or a commercial inactivated FMDV vaccine.Conclusions: This study demonstrates the potential of the HEV truncated capsid protein VLPs as a presenting-platform of exogenous antigens in vaccine design and promising preliminary results on chimeric VLP vaccine against foot-and-mouth disease.

scholarly journals Chimeric hepatitis E virus-like particle as a carrier for oral-delivery

Vaccine ◽  
2013 ◽  
Vol 31 (2) ◽  
pp. 417-424 ◽  
Author(s):  
Pitchanee Jariyapong ◽  
Li Xing ◽  
Nienke E. van Houten ◽  
Tian-Cheng Li ◽  
Wattana Weerachatyanukul ◽  
...  
Keyword(s):  
Oral Delivery ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particle

scholarly journals Structure of the hepatitis E virus-like particle suggests mechanisms for virus assembly and receptor binding

2009 ◽  
Vol 106 (31) ◽  
pp. 12992-12997 ◽  
Author(s):  
T. S. Y. Guu ◽  
Z. Liu ◽  
Q. Ye ◽  
D. A. Mata ◽  
K. Li ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Hepatitis E Virus ◽  
Virus Assembly ◽  
Hepatitis E ◽  
Virus Like Particle

scholarly journals Crystallization and structure determination of recombinant hepatitis E virus-like particle

2008 ◽  
Vol 64 (a1) ◽  
pp. C327-C327
Author(s):  
N. Miyazaki ◽  
C.-Y. Wang ◽  
A. Higashiura ◽  
A. Nakagawa ◽  
T.-C. Li ◽  
...  
Keyword(s):  
Structure Determination ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
Virus Like Particle

Empty virus-like particle-based enzyme-linked immunosorbent assay for antibodies to hepatitis E virus

2000 ◽  
Vol 62 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Tian-Cheng Li ◽  
Jing Zhang ◽  
Haruhide Shinzawa ◽  
Masamichi Ishibashi ◽  
Michio Sata ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Like Particle

Hepatitis E Virus in LeberBiopsien von Patienten mit akuter Hepatitis unbekannter Ätiologie

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
C Dorloff ◽  
J Hemberger ◽  
M Odenthal ◽  
H Holzmann ◽  
S Aberle ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E

Severe course of Hepatitis E Virus infection in a patient with pre-existing autoimmune liver disease

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
S Schlosser ◽  
J Pflaum ◽  
K Weigand ◽  
JJ Wenzel ◽  
W Jilg ◽  
...  
Keyword(s):  
Liver Disease ◽  
Virus Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Autoimmune Liver Disease
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