scholarly journals Isolation and Characterization of a Novel Toluene-Degrading Bacterium Exhibiting Potential Application in Bioremediation

2013 ◽  
Author(s):  
Afrouzossadat Hosseini Abari ◽  
Giti Emtiazi ◽  
Seyed Mahdi Ghasemi ◽  
Rasoul Roghanian
Keyword(s):  
Potential Application ◽  
Degrading Bacterium ◽  
Isolation And Characterization

The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host

2021 ◽  
Vol 97 (4) ◽  
Author(s):  
Rute Ferreira ◽  
Rui Amado ◽  
Jorge Padrão ◽  
Vânia Ferreira ◽  
Nicolina M Dias ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Communities ◽  
Potential Application ◽  
Deleterious Effect ◽  
Wastewater Treatment Plants ◽  
Lytic Phage ◽  
Filamentous Bacterium ◽  
Sphaerotilus Natans ◽  
Isolation And Characterization

ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.

scholarly journals Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3

2019 ◽  
Vol 51 (2) ◽  
pp. 170-178
Author(s):  
Tao Peng ◽  
Qing-Kun Chen ◽  
Jing-Sheng Lun ◽  
Amit Pratush ◽  
Guang-Ming Xiong ◽  
...  
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

2005 ◽  
Vol 251 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Li Yang ◽  
Yu-hua Zhao ◽  
Bing-xin Zhang ◽  
Ching-Hong Yang ◽  
Xin Zhang
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of a Novel p,p’-DDT-Degrading Bacterium: Aeromonas sp. Strain MY1 from Agricultural Soil

2020 ◽  
pp. 12-22
Author(s):  
Y. Murtala ◽  
B. C. Nwanguma ◽  
L. U. S. Ezeanyika
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Phylogenetic Analyses ◽  
Inhibitory Effect ◽  
Aerobic Conditions ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Background: Despite the banned on the use of dichlorodiphenyltrichloroethane (DDT) and other Persistent Organic Pollutants (POPs) by the Stockholm Convention for their toxicity, emerging shreds of evidence have indicated that DDT is, however, still in use in developing countries. This might increase the global burden of DDT contamination and its hazardous effects. Aim: This study focused on the isolation and characterization of p,p’-DDT-degrading bacterium from a tropical agricultural soil. Methodology: Standard isolation procedure was used for the screening and isolation of the strain. The 16S rRNA and phylogenetic analyses were used to identify the isolate and established protocols were followed to characterize the strain. Results: A new strain belonging to the genus Aeromonas was isolated from agricultural soil using minimal salt-p,p’-DDT enrichment medium. The 16S rRNA sequencing was used to identify the strain and the partial sequence was deposited in the NCBI GenBank as Aeromonas sp. Strain MY1. This mesophilic isolate was capable of utilizing up to 50 mgL-1 of p,p’-DDT as the sole carbon source at an optimum pH of 7.5 and optimum temperature of 35 °C within 120 h under aerobic conditions. Fe2+ (0.2 mgL-1) demonstrated a stimulatory effect on the p,p’-DDT degradation capacity by the strain MY1. However, Zn, Cu, Pb, Hg, Ag and Cr ions have demonstrated various patterns of inhibitory effect on the p,p’-DDT degradation capacity of the isolate at 0.2 mgL-1. The strain MY1 could be a promising candidate for the bioremediation of p,p’-DDT contaminant. Conclusion: Aeromonas sp. strain MY1 was capable of utilizing p,p’-DDT as a sole carbon source under aerobic conditions. The utilization capacity of the strain was influenced by some heavy metals. Fe was found to enhance the p,p’-DDT utilization capacity of the isolate at a lower concentration. While Zn, Cu, Pb, Hg, Ag and Cr showed various patterns of inhibitory effect.

Isolation and characterization of an electrochemically active and cyanide-degrading bacterium isolated from a microbial fuel cell

RSC Advances ◽  
2014 ◽  
Vol 4 (69) ◽  
pp. 36458 ◽  
Author(s):  
Feng Ya-li ◽  
Wang Wei-da ◽  
Tang Xin-hua ◽  
Li Hao-ran ◽  
Du Zhuwei ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Electrochemically Active
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