scholarly journals Molecular Characterization of Leishmania Infection in Sand flies From Sistan Va Baluchistan Province, Southeastern Iran

2012 ◽  
Vol 5 (2) ◽  
pp. 430-431 ◽  
Author(s):  
Hamid Kassiri ◽  
Saied Reza Naddaf ◽  
Mehdi Mohebali ◽  
Ezat-Aldin Javadian
2019 ◽  
Vol 7 (12) ◽  
pp. 644
Author(s):  
Mattia Calzolari ◽  
Elena Carra ◽  
Gianluca Rugna ◽  
Paolo Bonilauri ◽  
Federica Bergamini ◽  
...  

Visceral leishmaniasis (VL) caused by Leishmania (L.) infantum is a public health threat in the Emilia-Romagna region, northeastern Italy, but its epidemiology has not been fully elucidated in this area. The objective of this study was to characterize Leishmania infection in sand flies collected in a re-emerging focus of VL in the Bologna province. During the summer of 2016, 6114 sand flies were collected, identified, and tested for Leishmania detection. Of the identified sand flies, 96.5% were Phlebotomus (P.) perfiliewi and 3.5% were P. perniciosus. Detected parasites were characterized by biomolecular methods (multilocus microsatellite typing and characterization of repetitive region on chromosome 31), and quantified by real-time PCR. The prevalence of Leishmania infection in individually-tested P. perfiliewi sand flies varied from 6% to 10% with an increasing trend during the season. Promastigotes of L. infantum were isolated by dissection in one P. perfiliewi female; the isolated strain (Lein-pw) were closely related to Leishmania parasites from VL cases in northeastern Italy, but differed from strains isolated in dogs from the same area. Our findings strongly support the vector status of P. perfiliewi for human VL in the study area.


2009 ◽  
Vol 56 (6) ◽  
pp. 583-588 ◽  
Author(s):  
JAN VOTÝPKA ◽  
LUCIE LANTOVÁ ◽  
KASHINATH GHOSH ◽  
HENK BRAIG ◽  
PETR VOLF

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom

2012 ◽  
Vol 224 (03) ◽  
Author(s):  
A Streltsov ◽  
S Emmrich ◽  
F Engeland ◽  
JH Klusmann

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