Evaluation of Antimicrobial Effect of Ferulago angulata on Staphylococcus aureus Growth Isolated From Subclinical Mastitis, in Vitro Study

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Somayeh Bohlouli ◽  
Sara Hasanvand
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Subclinical Mastitis ◽  
Alcoholic Extract ◽  
Causative Agents ◽  
Disk Method ◽  
Bacterial Effect

Background: Staphylococcus aureus is one of the important causative agents in mastitis. Objectives: The aim of this study was determination antimicrobial effect of Ferulago angulata and different common therapeutic antibiotics on growth of Staphylococcus aureus isolated from subclinical mastitis cases. Methods: In this study the antibacterial effect of Ferulago angulata essential oil, aqueous and hydro alcoholic extract on Staphylococcus aureus growth was done and compared with common therapeutic antibiotics such as tetracycline, erythromycin, kanamycin and gentamicin by diffusion disk method. Minimum inhibitory concentration and minimum bactericidal concentration were tested through tube standard method. Results: Antimicrobial effect of essential oil and hydro alcoholic extract was increased by dose-dependently. 40 mg/mL extract and 20% essential oil are the highest dose with antimicrobial effect. Extract of Ferulago angulata has maximum antibacterial activity on the growth of S. aureus. Conclusions: Ferulago angulata had remarkable anti-bacterial effect on Staphylococcus aureus growth isolated from subclinical mastitis cases. In comparison Ferulago angulata essential oil was more powerful than extract. Regarding the fact that antibiotic resistance is growing, Ferulago angulata as an herbal plant with anti-bacterial effect could be used in Staphylococcus aureus isolated from subclinical mastitis cases.

scholarly journals Chemical Composition and Antimicrobial Potential of Satureja hortensis L. in Fresh Cow Cheese

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Ersilia Alexa ◽  
Corina Danciu ◽  
Ileana Cocan ◽  
Monica Negrea ◽  
Adriana Morar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Chemical Composition ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Alcoholic Extract ◽  
Satureja Hortensis ◽  
Fresh Cheese ◽  
Vitro Effect

This study presents data about the chemical composition and antimicrobial effect of Satureja hortensis L. used as both dry plant and essential oil, on fresh cow’s cheese, in order to extend its shelf-life. The proximate and elemental composition of dry plant of Satureja hortensis L. highlights important level of microelements. The content of microelements increases even when small amounts of Satureja hortensis in fresh cheese were added. The addition of Satureja hortensis dry plant leads to an increase in Fe (13.46–65.54%) and Mn (8.33–88.33%) content of fresh cheese, depending on the amount of plant added. The composition of essential oil isolated from Satureja hortensis L. was analyzed by GC-MS and the main compounds found were carvacrol (19.68%), o-cymene (30.86%), and p-cymene (28.07%). In order to use Satureja hortensis L. as natural preservative in food industry, in vitro effect of plant extract and essential oil against Staphylococcus aureus Gram-positive bacteria was tested. The oil of Satureja hortensis L. showed antimicrobial activity at 0.50–1.5%, while the alcoholic extract does not inhibit Staphylococcus aureus mycelial growth. The antimicrobial effect of Satureja hortensis L. dry plant in various proportions (0.5–1.5%) and essential oil (0.1%; 0.25%; 0.5%), on fresh cow’s cheese, was assessed after 3 and 7 days by counting colonies obtained at 30°C. Results have shown that the addition of Satureja hortensis L. dry plant and essential oil led to a reduction in the total number of germs, this reduction being more significant when the essential oil was used. Regarding the effect of Satureja hortensis L. essential oil against Staphylococcus aureus inoculated in fresh cow’s cheese, the results highlight that the essential oil of Satureja hortensis L. may be a natural solution to prevent the development of this bacteria, while the ethanol extract does not prove to be effective.

scholarly journals The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study

2021 ◽  
Vol 55 (4) ◽  
pp. 285
Author(s):  
Bernadette Dian Novita ◽  
Silvia Sutandhio
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Water Extract ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Bark Extract ◽  
Native Plant ◽  
Cinnamomum Burmannii

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia

scholarly journals Comparative study of antimicrobial effect of brihati [solanum indicum linn ] root and fruit- an in vitro study.

2019 ◽  
Vol 7 (05) ◽  
Author(s):  
Sampada Dhongde ◽  
Shashidhar Naik
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Comparative Study ◽  
Wound Infection ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Literature Mining ◽  
Whole Plant

Brihati is a well known drug used in various diseases like dyspnoea, odema, wound infection etc1 .Both root and fruit are useful part of plant according to brihatrayi2-11.The study is undertaken for antimicrobial activity of root and fruit against Staphylococcus aureus and Staphylococcus Caprae. If  antimicrobial  effect of root and fruit is similar than it is our duty to save the plant. No  need to pluck the whole  plant or root and use only fruit extracts for antimicrobial activity.However the literature mining reveled that brihati have not been studied for antimicrobial activities, hence the present study is first systematic attempt to analyze the antimicrobial potential of Brihati on selected strains.

scholarly journals Antibacterial Effects of Hydro-alcoholic Extract of Pennyroyal, Cinnamon and Rhubarb on Klebsiella pneumoniae and Staphylococcus aureus: An In-vitro Study

2019 ◽  
pp. 1-6 ◽  
Author(s):  
Zohreh Gheisari ◽  
Leila Kalani ◽  
Mansoor Khaledi ◽  
Ali Nouri ◽  
Yaser Eshaghi Milasi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Extraction Process ◽  
Alcoholic Extract ◽  
Cinnamon Extract ◽  
Antimicrobial Metabolites ◽  
And Staphylococcus Aureus

Background: Medicinal plants have been used for many different purposes in the past. These plants may produce antimicrobial metabolites that affect the natural growth of microorganisms. The aim of this research was to study the antibacterial properties of rhubarb, cinnamon and pennyroyal on Staphylococcus aureus and Klebsiella pneumoniae. Materials and Methods: In this study, the effect of hydro-alcoholic extract of pennyroyal, cinnamon and rhubarb on Staphylococcus aureus and Klebsiella pneumoniae was investigated after their collection and extraction process. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of this extract on bacteria were also measured. Results: The MIC of hydro alcoholic extract of pennyroyal was above 400 mg/ml for both Klebsiella pneumoniae and Staphylococcus aureus. Also the MBC has been reported 400 and 500 mg/dL, respectively. The MIC of cinnamon extract for Klebsiella pneumoniae and Staphylococcus aureus were reported 250 and higher and 500 and higher, respectively. Also, the MBC was reported 500 and 600 mg/ml, respectively. Klebsiella pneumoniae was resistant to rhubarb plant extract, and the MIC and the MBC for Staphylococcus aureus were reported 200 mg/ml and higher and 400 mg/ml and higher, respectively. Conclusion: The results of this study demonstrated that the hydro alcoholic extract of cinnamon, pennyroyal and rhubarb has anti bacterial effect against Klebsiella pneumoniae and Staphylococcus aureus.

scholarly journals The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study

2020 ◽  
Vol 55 (4) ◽  
pp. 285
Author(s):  
Bernadette Dian Novita ◽  
Silvia Sutandhio
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Water Extract ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Bark Extract ◽  
Native Plant ◽  
Cinnamomum Burmannii

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia.

scholarly journals Comparing Anti-bacterial Effect of CO2 Laser and 0.2% Chlorhexidine Solution on Staphylococcus aureus and Pseudomonas aeruginosa: In Vitro Study

2016 ◽  
Vol 7 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Ali Taghavi ◽  
Afsoon Asadollahi ◽  
Hosein Eslami ◽  
Rana Attaran ◽  
Mohammad Reza Ranjkesh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Co2 Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Chlorhexidine Solution ◽  
Bacterial Effect
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