scholarly journals Prokaryotic Expression and Purification of Recombinant Human Leukemia Inhibitory Factor; Analysis of the Ability to Maintain Pluripotency in Embryonic Stem Cells

2018 ◽  
Vol 20 (8) ◽  
Author(s):  
Behrouz Taheri ◽  
Mohammad-Hossein Modarressi ◽  
Mahmoud Hashemitabar ◽  
Mohammad Miryounesi ◽  
Elahe Motevaseli
Keyword(s):  
Stem Cells ◽  
Factor Analysis ◽  
Embryonic Stem Cells ◽  
Leukemia Inhibitory Factor ◽  
Prokaryotic Expression ◽  
Embryonic Stem ◽  
Inhibitory Factor ◽  
Human Leukemia ◽  
Expression And Purification ◽  
Prokaryotic Expression And Purification

scholarly journals Developmental expression of myeloid leukemia inhibitory factor gene in preimplantation blastocysts and in extraembryonic tissue of mouse embryos.

1990 ◽  
Vol 10 (7) ◽  
pp. 3801-3805 ◽  
Author(s):  
F Conquet ◽  
P Brûlet
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Growth Factor ◽  
Leukemia Inhibitory Factor ◽  
Embryonic Stem ◽  
Inhibitory Factor ◽  
Developmental Expression ◽  
Mouse Development ◽  
Extraembryonic Tissue ◽  
Developmental Potential

Murine leukemia inhibitory factor (LIF) protein is a growth factor which has the ability to maintain the developmental potential of pluripotent embryonic stem cells through a specific receptor. We have examined the expression pattern of the LIF gene from the preimplantation stage (3.5 days post coitum) to the midgestation stage (12.5 days post coitum) of the mouse embryo. LIF transcripts were detected at the preimplantation blastocyst stage, whereas no transcripts were detectable in embryonic stem cells. LIF gene transcription continued in the extraembryonic tissue of the 7.5-day and in the placenta of 9.5-, 10.5-, and 12.5-day post coitum embryos. No transcripts were detected in the embryo proper of the corresponding stages. Our results suggest that this growth factor is synthesized in the extraembryonic part of the embryo and acts on the embryonic tissues during early mouse development.

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