scholarly journals Leukocyte and Platelet Rich Plasma (L-PRP) Versus Leukocyte and Platelet Rich Fibrin (L-PRF) For Articular Cartilage Repair of the Knee: A Comparative Evaluation in an Animal Model

2015 ◽  
Vol 17 (10) ◽  
Author(s):  
Davoud Kazemi ◽  
Ashraf Fakhrjou
Keyword(s):  
Animal Model ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Comparative Evaluation ◽  
Platelet Rich Plasma ◽  
Articular Cartilage Repair ◽  
Platelet Rich Fibrin

scholarly journals Reprogrammed Synovial Fluid-Derived Mesenchymal Stem/Stromal Cells Acquire Enhanced Therapeutic Potential for Articular Cartilage Repair

Cartilage ◽  
2021 ◽  
pp. 194760352110408
Author(s):  
Brian E. Walczak ◽  
Hongli Jiao ◽  
Ming-Song Lee ◽  
Wan-Ju Li
Keyword(s):  
Animal Model ◽  
Articular Cartilage ◽  
Synovial Fluid ◽  
Cartilage Repair ◽  
Stromal Cells ◽  
Cartilage Damage ◽  
Cellular Reprogramming ◽  
Human Mscs ◽  
Articular Cartilage Repair

Objectives Functions of mesenchymal stem/stromal cells (MSCs) are affected by patient-dependent factors such as age and health condition. To tackle this problem, we used the cellular reprogramming technique to epigenetically alter human MSCs derived from the synovial fluid of joints with osteoarthritis (OA) to explore the potential of reprogrammed MSCs for repairing articular cartilage. Materials and Methods MSCs isolated from the synovial fluid of three patients’ OA knees (Pa-MSCs) were reprogrammed through overexpression of pluripotency factors and then induced for differentiation to establish reprogrammed MSC (Re-MSC) lines. We compared the in vitro growth characteristics, chondrogenesis for articular cartilage chondrocytes, and immunomodulatory capacity. We also evaluated the capability of Re-MSCs to repair articular cartilage damage in an animal model with spontaneous OA. Results Our results showed that Re-MSCs increased the in vitro proliferative capacity and improved chondrogenic differentiation toward articular cartilage-like chondrocyte phenotypes with increased THBS4 and SIX1 and decreased ALPL and COL10A1, compared to Pa-MSCs. In addition, Re-MSC-derived chondrocytes expressing elevated COL2A and COL2B were more mature than parental cell-derived ones. The enhancement in chondrogenesis of Re-MSC involves the upregulation of sonic hedgehog signaling. Moreover, Re-MSCs improved the repair of articular cartilage in an animal model of spontaneous OA. Conclusions Epigenetic reprogramming promotes MSCs harvested from OA patients to increase phenotypic characteristics and gain robust functions. In addition, Re-MSCs acquire an enhanced potential for articular cartilage repair. Our study here demonstrates that the reprogramming strategy provides a potential solution to the challenge of variation in MSC quality.

Platelet-rich Plasma for Articular Cartilage Repair

2013 ◽  
Vol 21 (4) ◽  
pp. 213-219 ◽  
Author(s):  
Geoffrey D. Abrams ◽  
Rachel M. Frank ◽  
Lisa A. Fortier ◽  
Brian J. Cole
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Platelet Rich Plasma ◽  
Articular Cartilage Repair

Platelet-rich plasma – A promising cell carrier for micro-invasive articular cartilage repair

2009 ◽  
Vol 72 (4) ◽  
pp. 455-457 ◽  
Author(s):  
Wei Wu ◽  
Junrui Zhang ◽  
Qinshan Dong ◽  
Yanpu Liu ◽  
Tianqiu Mao ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Platelet Rich Plasma ◽  
Articular Cartilage Repair ◽  
Cell Carrier

scholarly journals Statistical image analysis and escort histograms in characterization of articular cartilage repair in a skeleton animal model

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252505
Author(s):  
Ryszard Tomaszewski ◽  
Jerzy Dajka
Keyword(s):  
Animal Model ◽  
Image Analysis ◽  
Articular Cartilage ◽  
Medical Treatment ◽  
Cartilage Repair ◽  
Cluster Formation ◽  
Growth Plate Chondrocytes ◽  
Articular Cartilage Repair ◽  
Statistical Image Analysis ◽  
Significant Difference

Statistical image analysis of an ensemble of digital images of histological samples is performed as an auxiliary investigation a result of the recently proposed method of articular cartilage repair utilizing growth plate chondrocytes in a skeleton animal model. A fixed–shift model of maximal likelihood estimates of image histograms applied for monochromatic (grayscale) images or their RGB components confirms the statistically significant effect of the previously proposed medical treatment. The type of staining used to prepare images of histological samples is related to the visibility of the effectiveness of medical treatment. Hellinger distance of escort distributions for maximal likelihood estimates of image histograms of medically treated and control samples is investigated to identify grayscale (or RGB) intensities responsible for statistically significant difference of the estimates. A difference of Shannon entropy quantifying informational content of the histograms allows one to identify staining and image colors which are most suitable to visualize cluster formation typical for articular cartilage repair processes.

Small-Diameter Awls Improve Articular Cartilage Repair After Microfracture Treatment in a Translational Animal Model

2015 ◽  
Vol 44 (1) ◽  
pp. 209-219 ◽  
Author(s):  
Patrick Orth ◽  
Julia Duffner ◽  
David Zurakowski ◽  
Magali Cucchiarini ◽  
Henning Madry
Keyword(s):  
Animal Model ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Small Diameter ◽  
Articular Cartilage Repair

Articular Cartilage Repair Strategies in the Ankle Joint

2007 ◽  
pp. 283-309 ◽  
Author(s):  
Monika Volesky ◽  
Timothy Charlton ◽  
Jonathan T. Deland
Keyword(s):  
Articular Cartilage ◽  
Ankle Joint ◽  
Cartilage Repair ◽  
Articular Cartilage Repair
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