scholarly journals Molecular Detection of Metallo-β-lactamase Genes blaIMP, blaVim,‎ ‎and blaSPM-1‎ in Bacillus cereus Strains Isolated from Infant Milk Powder Samples

2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Ciamak Ghazaei
Keyword(s):  
Bacillus Cereus ◽  
Molecular Detection ◽  
Milk Powder ◽  
Powder Samples ◽  
Infant Milk Powder

Evaluation of Bacterial Contamination in Goat Milk Powder Using PacBio Single Molecule Real-Time Sequencing and Droplet Digital PCR

2018 ◽  
Vol 81 (11) ◽  
pp. 1791-1799 ◽  
Author(s):  
HUIMIN MA ◽  
JING LI ◽  
XIAOXIA XI ◽  
HAIYAN XU ◽  
LAGA WURI ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Single Molecule ◽  
Bacterial Contamination ◽  
Bacterial Species ◽  
Milk Powder ◽  
Goat Milk ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Powder Samples

ABSTRACT Goat milk powder is a nutritious and easy-to-store product that is highly favored by consumers. However, the presence of contaminating bacteria and their metabolites may significantly affect the flavor, solubility, shelf life, and safety of the product. To comprehensively and accurately understand the sanitary conditions in the goat milk powder production process and potential threats from bacterial contamination, a combination of Pacific Biosciences single molecule real-time sequencing and droplet digital PCR was used to evaluate bacterial contamination in seven goat milk powder samples from three dairies. Ten phyla, 119 genera, and 249 bacterial species were identified. Bacillus, Paenibacillus, Lactococcus, and Cronobacter were the primary genera. Bacillus cereus, Lactococcus lactis, Alkaliphilus oremlandii, and Cronobacter sakazakii were the dominant species. With droplet digital PCR, 6.3 × 104 copies per g of Bacillus cereus and 1.0 × 104 copies per g of Cronobacter spp. were quantified, which may increase the risk of food spoilage and the probability of foodborne illness and should be monitored and controlled. This study offers a new approach for evaluating bacterial contamination in goat milk powder and supplies a reference for the assessment of food safety and control of potential risk, which will be of interest to the dairy industry.

Simultaneous Determination of 2- and 3-MCPD Esters in Infant Formula Milk Powder by Solid-Phase Extraction and GC-MS Analysis

2016 ◽  
Vol 99 (3) ◽  
pp. 786-791 ◽  
Author(s):  
Liyuan Wang ◽  
Ying Ying ◽  
Zhengyan Hu ◽  
Tianjiao Wang ◽  
Xianghong Shen ◽  
...  
Keyword(s):  
Infant Formula ◽  
Solid Phase ◽  
Milk Powder ◽  
Fatty Acid Esters ◽  
Formula Milk ◽  
Ms Analysis ◽  
Infant Formula Milk ◽  
Powder Samples ◽  
Infant Milk Powder ◽  
Mcpd Esters

Abstract The objective of this study was to establish a method for detecting fatty acid esters of chloropropanols [including 2-monochloropropane-1,3-diol (2-MCPD) ester and 3-monochloropropane-1,2-diol (3-MCPD) ester] in infant milk powder using isotope dilution and GC-MS. The fat fraction in infant milk powder samples was extracted and treated with sodium methylate–methanol to cleave the ester bonds of the 2- and 3-MCPD esters. The resultant 2- and 3-MCPDs in the samples were purified with matrix solid-phase liquid–liquid extraction and derivatized with heptafluorobutyryl imidazole for GC-MS analysis. Standard samples of deuterium isotope–labeled 2- and 3-MCPD palmitic acid double esters and stearic acid double esters were used as the internal standards. We also detected 2- and 3-MCPD ester contents in 88 commercial samples of infant formula milk powder using this system. The detection system we established showed a good linearity of 2- and 3-MCPD ester contents in serially diluted standard solutions within the concentration range 25−1000 μg/L, with r2 > 0.9995 and an LOD of 30 μg/kg for both of the MCPD esters. The recoveries of the two MCPD esters spiked at 25, 50, 100, and 200 μg/kg in blank infant formula milk ranged from 98.2 to 110.5%, with RSDs <4.8%, suggesting good accuracy and reliability of this method. In the 88 commercial infant formula milk powder samples, the mean content of 2-MCPD ester was 41 μg/kg (0–52 μg/kg) and that of 3-MCPD was 185 μg/kg (0–316 μg/kg). Based on these results, we estimated the exposure levels of the two MCPD esters in infants fed on formula milk, and the results indicate potential health risks of consuming formula milk products contaminated by the two MCPD esters.

Occurence of potentially enterotoxigenic Bacillus cereus in infant milk powder

2013 ◽  
Vol 237 (2) ◽  
pp. 275-279 ◽  
Author(s):  
Angela Di Pinto ◽  
Elisabetta Bonerba ◽  
Giancarlo Bozzo ◽  
Edmondo Ceci ◽  
Valentina Terio ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Milk Powder ◽  
Infant Milk Powder

scholarly journals A Novel Graphene Oxide-Based Aptasensor for Amplified Fluorescent Detection of Aflatoxin M1 in Milk Powder

Sensors ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3840 ◽  
Author(s):  
Xiaodong Guo ◽  
Fang Wen ◽  
Qinqin Qiao ◽  
Nan Zheng ◽  
Matthew Saive ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Milk Powder ◽  
High Sensitivity ◽  
High Specificity ◽  
Fluorescent Detection ◽  
Aflatoxin M1 ◽  
Powder Samples ◽  
Infant Milk Powder

In this paper, a rapid and sensitive fluorescent aptasensor for the detection of aflatoxin M1 (AFM1) in milk powder was developed. Graphene oxide (GO) was employed to quench the fluorescence of a carboxyfluorescein-labelled aptamer and protect the aptamer from nuclease cleavage. Upon the addition of AFM1, the formation of an AFM1/aptamer complex resulted in the aptamer detaching from the surface of GO, followed by the aptamer cleavage by DNase I and the release of the target AFM1 for a new cycle, which led to great signal amplification and high sensitivity. Under optimized conditions, the GO-based detection of the aptasensor exhibited a linear response to AFM1 levels in a dynamic range from 0.2 to 10 μg/kg, with a limit of detection (LOD) of 0.05 μg/kg. Moreover, the developed aptasensor showed a high specificity towards AFM1 without interference from other mycotoxins. In addition, the technique was successfully applied for the detection of AFM1 in infant milk powder samples. The aptasensor proposed here offers a promising technology for food safety monitoring and can be extended to various targets.

scholarly journals Evaluation of some chemical parameters of powder milk available in Mymensingh town

2012 ◽  
Vol 10 (1) ◽  
pp. 95-100 ◽  
Author(s):  
M F I Kajal ◽  
A Wadud ◽  
M N Islam ◽  
P K Sarma
Keyword(s):  
Statistical Analysis ◽  
Significant Variation ◽  
Milk Powder ◽  
Cow Milk ◽  
Local Market ◽  
Chemical Parameters ◽  
Total Solids ◽  
Milk Samples ◽  
Powder Milk ◽  
Powder Samples

The study was undertaken to evaluate some chemical parameters of powder milk available in Mymensingh town. Powder milk samples of six different brands namely Kwality, NIDO, Diploma, Anchor, Farmland & Starship were collected from local market. Powder samples were analyzed to know the chemical (PH, acidity, fat, protein, lactose, ash, moisture, solids-not-fat, total solids) characteristics. PH, acidity of all samples was almost similar to the cow milk  when the dried milk was reconstituted. PH ranged from 6.6-6.8, acidity from 0.10-0.15, fat from 26.198-27.89 g/100 g,  protein from 25.22-27.01 g/100g, lactose from 36.63-37.65 g/100g, ash from 5.34-5.48 g/100g, moisture from 3.36-4.48 g/100g, solids-not-fat from 67.99-70.07 g/100g, total solids from 95.51-96.63 g/100g, among the brands of powder milk. Statistical analysis showed significant variation for (PH and acidity) among different powder milk brands. chemical parameters in all brands of milk powder was found as per recommended standard. DOI: http://dx.doi.org/10.3329/jbau.v10i1.12099 J. Bangladesh Agril. Univ. 10(1): 95–100, 2012    

Chemometrics-assisted liquid chromatography-full scan mass spectrometry for simultaneous determination of multi-class estrogens in infant milk powder

2018 ◽  
Vol 10 (12) ◽  
pp. 1459-1471 ◽  
Author(s):  
Xiao-Dong Sun ◽  
Hai-Long Wu ◽  
Zhi Liu ◽  
Li-Xia Xie ◽  
Yong Hu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Milk Powder ◽  
Calibration Method ◽  
Second Order ◽  
Infant Milk Powder ◽  
Full Scan ◽  
Second Order Calibration

Simultaneous determination of estrogens in infant milk powder using a second-order calibration method coupled with LC-MS operated in full scan mode.

scholarly journals Salmonella Detection in Dried Milk Products by Motility Enrichment on Modified Semisolid Rappaport-Vassiliadis Medium: Collaborative Study

1996 ◽  
Vol 79 (2) ◽  
pp. 441-450 ◽  
Author(s):  
Robert F Bolderduk ◽  
John E Milas ◽  
H Asperger ◽  
H Becker ◽  
S Chatron ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Milk Powder ◽  
Culture Method ◽  
Milk Products ◽  
Whey Powder ◽  
Whole Milk ◽  
Significant Difference ◽  
Powder Samples ◽  
Butter Milk ◽  
Whole Milk Powder

Abstract A collaborative study involving 19 laboratories was performed to validate motility enrichment on modified semisolid Rappaport-Vassiliadis (MSRV) medium for rapid detection of motile Salmonella in dried milk products. The MSRV method was compared with the AOAC culture method for detection of Salmonella in nonfat milk powder, whole milk powder, whey powder, casein powder, and butter milk powder. Samples were artificially inoculated with Salmonella at 2 levels of contamination. Un-inoculated control samples were included for each type of product. The sensitivity rates were 100% for the MSRV method and 99.0% for the AOAC culture method, while the specificity rate was 100.0% for both methods. Only for the samples of whey powder, which were inoculated with H2S negative S. tennessee, was there a significant difference in the proportion of samples positive by MSRV and the culture procedure. The MSRV method for detection of motileSalmonella in dried milk products has been adopted first action by AOAC INTERNATIONAL.

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