scholarly journals Evaluation of Drug Resistance Before and After Biofilm Formation of Bacteria Causing Wound Infection and Detection of Their Protease Activity

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Tasnuba Tabassum Proma ◽  
Tasnia Ahmed
Keyword(s):  
Wound Infection ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Alcaligenes Faecalis ◽  
Protease Production ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Before And After ◽  
Almost All

Background: Wound infection is a highly common problem in hospital settings, where microbes are often resistant and difficult to treat due to rapid exposure to antibiotics. While treating wound infection, bacteria often enter the deep tissue; as therapy needs long exposure time, bacteria have sufficient time to develop biofilm, which makes them much more resistant to antibiotics. Objectives: The current study was performed to identify wound-infecting bacteria and determine their protease production activity. Methods: The ability to produce biofilm was evaluated by the Congo red agar and tube methods. Antibiotic resistance pattern was assessed before and after biofilm formation to detect the changes in resistance due to biofilm formation. Results: We identified Pseudomonas aeruginosa, Proteus mirabilis, Proteus vulgaris, Corynebacteriumxerosis., Alcaligenes faecalis, Bacillus cereus, Escherichia coli, Acinetobacterspp., Klebsiellapneumonia, Staphylococcus spp., Shigella spp., and Salmonella spp. in 20 wound samples, among which about 10 isolates were found to be biofilm producers. Almost all the biofilm producers showed complete resistance or a much smaller inhibition zone. Conclusions: Pathogenic bacteria can be more difficult to eradicate by antibiotic treatment if they are able to produce biofilm; thus, it is essential to prevent biofilm formation.

scholarly journals Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk

Biologics ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 164-176
Author(s):  
Abdallah S. Abdelsattar ◽  
Anan Safwat ◽  
Rana Nofal ◽  
Amera Elsayed ◽  
Salsabil Makky ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Salmonella Spp ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Wide Range ◽  
Almost All ◽  
And Control ◽  
Milk And Dairy Products

Food safety is very important in the food industry as most pathogenic bacteria can cause food-borne diseases and negatively affect public health. In the milk industry, contamination with Salmonella has always been a challenge, but the risks have dramatically increased as almost all bacteria now show resistance to a wide range of commercial antibiotics. This study aimed to isolate a bacteriophage to be used as a bactericidal agent against Salmonella in milk and dairy products. Here, phage ZCSE6 has been isolated from raw milk sample sand molecularly and chemically characterized. At different multiplicities of infection (MOIs) of 0.1, 0.01, and 0.001, the phage–Salmonella interaction was studied for 6 h at 37 °C and 24 h at 8 °C. In addition, ZCSE6 was tested against Salmonella contamination in milk to examine its lytic activity for 3 h at 37 °C. The results showed that ZCSE6 has a small genome size (<48.5 kbp) and belongs to the Siphovirus family. Phage ZCSE6 revealed a high thermal and pH stability at various conditions that mimic milk manufacturing and supply chain conditions. It also demonstrated a significant reduction in Salmonella concentration in media at various MOIs, with higher bacterial eradication at higher MOI. Moreover, it significantly reduced Salmonella growth (MOI 1) in milk, manifesting a 1000-fold decrease in bacteria concentration following 3 h incubation at 37 °C. The results highlighted the strong ability of ZCSE6 to kill Salmonella and control its growth in milk. Thus, ZCSE6 is recommended as a biocontrol agent in milk to limit bacterial growth and increase the milk shelf-life.

scholarly journals Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253445
Author(s):  
Chee-Hoo Yip ◽  
Sobina Mahalingam ◽  
Kiew-Lian Wan ◽  
Sheila Nathan
Keyword(s):  
Staphylococcus Aureus ◽  
Liquid Chromatography ◽  
Virulence Factors ◽  
Bacterial Growth ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Bacterial Virulence ◽  
Protease Production ◽  
Natural Soil ◽  
Microbial Competition

Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens, particularly in microbial competition through its antimicrobial activity, towards the growth and secreted virulence factors of four clinical pathogenic bacteria (methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa) as well as Staphylococcus aureus and Escherichia coli. Prodigiosin was first extracted from S. marcescens and its purity confirmed by absorption spectrum, high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The extracted prodigiosin was antagonistic towards all the tested bacteria. A disc-diffusion assay showed that prodigiosin is more selective towards Gram-positive bacteria and inhibited the growth of MRSA, S. aureus and E. faecalis and Gram-negative E. coli. A minimum inhibitory concentration of 10 μg/μL of prodigiosin was required to inhibit the growth of S. aureus, E. coli and E. faecalis whereas > 10 μg/μL was required to inhibit MRSA growth. We further assessed the effect of prodigiosin towards bacterial virulence factors such as haemolysin and production of protease as well as on biofilm formation. Prodigiosin did not inhibit haemolysis activity of clinically associated bacteria but was able to reduce protease activity for MRSA, E. coli and E. faecalis as well as decrease E. faecalis, Salmonella Typhimurium and E. coli biofilm formation. Results of this study show that in addition to its role in inhibiting bacterial growth, prodigiosin also inhibits the bacterial virulence factor protease production and biofilm formation, two strategies employed by bacteria in response to microbial competition. As clinical pathogens were more resistant to prodigiosin, we propose that prodigiosin is physiologically important for S. marcescens to compete against other bacteria in its natural soil and surface water environments.

scholarly journals Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Salmonella Spp ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

scholarly journals Effect of Lactobacillus salivarius supernatant against growth and biofilm formation of some pathogenic microorganisms

2016 ◽  
Vol 13 (2) ◽  
pp. 247-252
Author(s):  
Baghdad Science Journal
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Pathogenic Microorganisms ◽  
Lactobacillus Salivarius ◽  
Inhibition Effect ◽  
Probiotic Lactobacillus ◽  
Diffusion Assay ◽  
Klebsiella Spp

The Inhbititory effect of cocentrated and non-cocentrated supernatant of the probiotic Lactobacillus salivarius against growth of some potential pathogenic microorganisms which included Pseudomonas eruginosa, Klebsiella spp, Escherichia coli and Candida albicans. The results were diffusion assay revealed that concentrated and non-concentrated supernatant had inhitory effect against pathogenic bacteria with inhibition zone renged between 13-17mm while inhibition effect of concentrated supernatant against C.albicans was inhibition zone 8mm. On the other hand, the effect of these suprnatant against biofilm formation of the tested microorganisms was studied. The result showed that the concentrated supernatant had inhibitory effect on biofilm formation for all tested microorganisms with percentage (28-29)% against tested bacteria and (23)% against C.albicans.

Assessment of the Microbiological Quality of Ready-To-Use Vegetables for Health-Care Food Services

1997 ◽  
Vol 60 (8) ◽  
pp. 954-960 ◽  
Author(s):  
JOSEPH A. ODUMERU ◽  
SHEILA J. MITCHELL ◽  
DAVID M. ALVES ◽  
JOHN A. LYNCH ◽  
ARLENE J. YEE ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Food Services ◽  
Salmonella Spp ◽  
E Coli ◽  
Vegetable Samples ◽  
Serotype O ◽  
Select Group ◽  
Before And After

The microbiological quality of ready-to-use (RTU) vegetables, including chopped lettuce, salad mix, carrot sticks, cauliflower florets, sliced celery, coleslaw mix, broccoli florets, and sliced green peppers was determined before and after processing. Microbial profiles were obtained 24 h after processing and on days 4, 7, and 11 after storage at 4 and 10°C to simulate temperature abuse. In addition, the microbial profiles of four RTU vegetables, coleslaw mix, salad mix, cauliflower florets, and sliced green peppers were determined 7 days after distribution to a select group of Ontario hospitals. RTU vegetables, with the exception of green peppers, showed up to a 1-log decrease in aerobic colony counts after processing. These counts increased to preprocessing levels after 4 days of storage at both 4 and 10°C. RTU vegetables stored at temperature abuse conditions (10°C)had significantly higher counts (P &lt; 0.001) on days 4 to 11 as compared to those stored at 4°C. Green peppers had the highest bacterial counts while cauliflower and chopped lettuce had the lowest counts at both storage temperatures (P &lt; 0.05). Increased levels of Listeria monocytogenes in RTU vegetables were associated with temperature abuse. Levels of &gt;100 MPN/g for L. monocytogenes were detected in 8 of 120 (6.7%) samples stored at 10°C but not in 175 samples stored at 4°C after 7 days (P &lt; 0.05). Overall, L. monocytogenes was detected in 13 of 120 (10.8%) RTU vegetables stored for up to 11 days at 10°C and 5 of 176 (2.8%) samples stored at 4°C (P &lt; 0.05). E. coli was detected in 2 of the 120 (1.7%) processed RTU vegetables after day 7 of storage at 10°C and 1 of the 65 (1.5%) unprocessed vegetables from the same batches of vegetables used for processing. This indicator organism was not detected in RTU vegetable samples stored at 4°C or in any of the RTU vegetable samples obtained from hospital coolers. Other pathogenic bacteria, such as Salmonella spp., Campylobacter spp., Yersinia enterocolitica (serotype O:3) and verocytotoxigenic E. coli (VTEC) were not detected in any of the RTU vegetables tested, Recommendations regarding processing, distribution, and storage of these products are presented.

scholarly journals INOVASI PEMANFAATAN EKSTRAK ALGA HIJAU Ulva sp dari PANTAI LUK,SUMBAWA SEBAGAI KANDIDAT ANTIBAKTERI TERHADAP Salmonella thypi dan Staphylococcus aureus

2020 ◽  
Vol 4 (3) ◽  
pp. 1-6
Author(s):  
Rian Adha Ardinata ◽  
Baso Manguntungi
Keyword(s):  
Staphylococcus Aureus ◽  
Green Algae ◽  
Extraction Method ◽  
Pathogenic Bacteria ◽  
Morphological Characteristics ◽  
Inhibition Zone ◽  
Zone Of Inhibition ◽  
Almost All ◽  
Sea Area ◽  
And Staphylococcus Aureus

Indonesia is a maritime country that has rich marine resourcesoverflow. One of them is algae, which are distributed in almost all watersIndonesia is included in the Luk sea area, Sumbawa. Wrong type of potential algaehas the most content of primary and secondary metabolite compounds is algaegreen. This research was conducted to determine the antibacterial activity of the extractrough green algae from Luk beach. This research includes identifying green algaebased on morphological characteristics, followed by extracting green algae usingThe maceration method using distilled water was previously carried out in three preparationssamples namely drying (27 ° C), heating (70-80 ° C) and cooling (-10 ° C),each tested with a concentration of 20%, 40%, 60%, 80% and 100% then testedin vitro against Salmonella thypi and Staphylococcus aureus. TestingGreen algae antibacterial shows a different zone of inhibition in each type of extractionand concentrations of both pathogenic bacteria. The greatest inhibition zone against s.thypi isby using the extraction method with a concentration of 60% drying and method100% heating, while the greatest against s.aureusi is by usingextraction method heating concentration 80%.

scholarly journals Anti-plasmid activity of Chlorpromazine in types of antibiotics resistant pathogenic bacteria

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Alaa Qasim ◽  
Muhsin Ayoub Essa
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Bacterial Strains ◽  
Before And After ◽  
Bacteria Resistance ◽  
New Strategies

This study was performed to evaluate the ability of chlorpromazine compound to cure the plasmids and remove the antibiotic resistance character of multidrug resistance Gram-negative pathogenic bacteria. The curing experiment was done by incubating bacterial strains in nutrient broth with sub MIC concentration of chlorpromazine, and the disc diffusion method was used before and after curing to evaluate antibiotic resistance pattern of bacteria. Synergetic test of chlorpromazine with antibiotics that the studied bacteria showed resistance toward also done. The results showed the ability of chlorpromazine to remove the plasmids from four bacterial genera out of five genera, where the most plasmid removal cases was with E.cloacae, P.aeruginosa, and P.merabilis, and the loss of antibiotics resistance was observed in the same bacterial genera, but P.merabilis showed the most antibiotics resistance losing by three antibiotics out of nine antibiotics that the bacteria were resistant to them before curing. As for the synergistic effect, the compound showed a synergism with only two antibiotic and with the three aforementioned bacterial genera. From results of this study we concluded the possibility of removing bacteria resistance to antibiotics by eliminating plasmids through using chlorpromazine, and the possibility of using this compound in synergism with antibiotics, this will open the way to find new strategies  to treat many diseases caused by resistant bacteria.

scholarly journals The inhibitory effect of aqueous and alcoholic extract of red pepper on some isolated pathogenic bacteria from different areas of human body

Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2270-2273
Author(s):  
Lina Qays Yaseen ◽  
Sura Hameed Nayyef ◽  
Nadia Ibraheem Salih
Keyword(s):  
Pathogenic Bacteria ◽  
Control Sample ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Red Pepper ◽  
Alcoholic Extract ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Alcohol Extract ◽  
Long Time

In most underdeveloped nations, traditional medicine, including herbal treatment, is still widely used. Due to the growth of antibiotic resistance, this study aims to use pepper as an anti-bacterial as alternative to antibiotics. Pepper is one of the most important plants used as a medicine for a long time in various countries and civilizations. This study aims to use pepper as an anti-bacterial in alternative to antibiotics. The current study included the inhibitory efficacy of aqueous and alcoholic red pepper extract on seven bacterial isolates: -Staphylococcus aureus, Streptococcus spp, Escherichia coli, Klebseilla spp, Salmonella spp, Pseudomonas aeruginosa, Proteus spp. These isolates were isolated from different pathologies and regions, and they were diagnosed according to the site of infection. Several antibiotics were also used as a standard control sample for germs. This study shows that the highest inhibitory Effect against E. coli bacterium, as the average inhibition diameter was about 16.5 mm, and it is an excellent inhibitory when compared to the antagonist's gentamicin and nitrofurantoin as it showed good inhibitory efficacy against the bacteria such as S. aureus P. aeruginosa, and Salmonella spp. While the effect on Klebsiella was equal, on the other hand, the aqueous extract did not show any efficacy against Proteus spp, as was shown in the results. The results also showed that Staph. Aureus bacteria were the most affected by the alcohol extract of the red pepper as it showed a high inhibition zone compared with the control sample tetracycline and nitrofurantoin. The plant's aqueous and alcoholic red pepper extracts were effective against the tested bacterial isolates. The plant's aqueous and alcoholic red pepper extract has good inhibitory efficacy against the studied bacterial isolates.

scholarly journals Antimicrobial Resistance Pattern against E. coli and Salmonella spp. in Environmental Effluents

2016 ◽  
Vol 5 (2) ◽  
pp. 52-58 ◽  
Author(s):  
MM Hassan ◽  
M Ahaduzzaman ◽  
M Alam ◽  
MS Bari ◽  
KB Amin ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
E Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
Veterinary Hospital

Hospitals (medical & veterinary) and slaughterhouse effluents were the most contaminating effluents and need to be paid more attention due to pathogenic bacteria related to animal and public health concern. Two bacterial isolates such as E. coli and Salmonella from six medical hospitals, five veterinary hospitals and five slaughter houses were isolated to find out the antibiotic resistance pattern by using disc diffusion method. The antibiotic resistance patterns of identified isolates showed that Ampicillin, Ciprofloxacin, Enrofloxacin, Pefloxacin, Colistin, Erythromycin, Oxytetracycline were 100%, Doxycycline was 83%, Gentamycin was 50% and Neomycin was 33% resistance to medical isolates and Ampicillin, Enrofloxacin, Pefloxacin and Erythromycin were 100%, Ciprofloxacin was 40%, Colistin was 60%, Doxycycline was 80%, Gentamycin was 20%; Neomycin and Oxytetracycline 80% resistance to veterinary hospital isolates and Ampicillin, Enrofloxacin, Ciprofloxacin, Pefloxacin, Colistin, Oxytetracycline, Gentamycin, Doxycycline and Erythromycin were 100% and Neomycin was 40% resistance to slaughter houses isolates of E. coli. The level of resistance of Salmonella positive isolates was found Ampicillin, Enrofloxacin, Pefloxacin, Gentamycin and Erythromycin to 100%, Ciprofloxacin was 67%, Oxytetracycline was 33% but Colistin and Neomycin was found sensitive to the isolates from both medical and veterinary hospital. Results indicated that hospitals and slaughter houses waste effluent has multiple-antibiotic resistance against E. coli and Salmonella.International Journal of Natural Sciences (2015), 5(2) 52-58

scholarly journals Investigation of Pathogenic Bacterial Transport by Waterbirds: A Case Study of Flooded and Non-Flooded Rice Systems in Mississippi

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1833
Author(s):  
Alexandra G. Firth ◽  
Beth H. Baker ◽  
John P. Brooks ◽  
Renotta Smith ◽  
Raymond B. Iglay ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
External Input ◽  
Rice Fields ◽  
Salmonella Spp ◽  
Fecal Indicator ◽  
Fecal Matter ◽  
Pathogen Indicators ◽  
Pathogen Transport ◽  
Before And After

Waterbirds may facilitate pathogen transport in rice fields from autumn to winter. This study investigated pathogen dynamics in two types of rice fields, those considered conventionally managed and a low-external-input-sustainable-agriculture (LEISA) rice system in the Mississippi Alluvial Valley, winter 2017–2018. In each system, fields were (1) left unflooded or (2) flooded from November to March. Fecal indicator and pathogenic bacteria (enterococci, Clostridium perfringens, Escherichia coli, Salmonella spp., and Campylobacter spp.) were quantified in soil before and after winter flooding, and bird fecal matter estimated on both farm types. Water samples were tested for enterococci, C. perfringens and E. coli before fields were drained. The LEISA flooded fields had greater detections of C. perfringens in the soil than conventional non-flooded fields. There was an observed decreasing trend of C. perfringens detection associated with lower bird abundances among treatment groups. All observed pathogen levels in both systems were below EPA standards. Results suggest that long-term waterbird stopovers can influence pathogen indicators in soil, but not at levels to threaten human and environmental health standards. Future studies should focus on long-term monitoring of pathogen introduction in rice fields that harbor wintering waterfowl or other waterbirds.

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