scholarly journals Construction of Plasmid Insulin Gene Vector Containing Metallothionein IIA (pcDNAMTChIns) and Carbohydrate Response Element (ChoRE), and Its Expression in NIH3T3 Cell Line

2012 ◽  
Vol 10 (3) ◽  
pp. 543-547 ◽  
Author(s):  
Hossein Piri ◽  
Bahram Kazemi ◽  
Mohsen Rezaei ◽  
Mojgan Bandehpour ◽  
Iraj Khodadadi ◽  
...  
Keyword(s):  
Cell Line ◽  
Insulin Gene ◽  
Nih3t3 Cell ◽  
Gene Vector ◽  
Response Element ◽  
Nih3t3 Cell Line

scholarly journals Senescence-like changes induced by expression of p21Waf1/Cip1 in NIH3T3 cell line

Cell Research ◽  
2002 ◽  
Vol 12 (3-4) ◽  
pp. 229-233 ◽  
Author(s):  
Xi CHEN ◽  
Wei ZHANG ◽  
Yun Fei GAO ◽  
Xiao Qin SU ◽  
Zhong He ZHAI
Keyword(s):  
Cell Line ◽  
Nih3t3 Cell ◽  
Nih3t3 Cell Line

scholarly journals A novel polypeptide from Cervus nippon Temminck proliferation of epidermal cells and NIH3T3 cell line.

2006 ◽  
Vol 53 (2) ◽  
pp. 395-397 ◽  
Author(s):  
Shu-Wen Guan ◽  
Leng-Xin Duan ◽  
Yuan-Yuan Li ◽  
Ben-Xiang Wang ◽  
Qiu-Li Zhou
Keyword(s):  
Cell Line ◽  
Dose Range ◽  
Cervus Nippon ◽  
Epidermal Cells ◽  
Nih3t3 Cell ◽  
Amino Acid Residues ◽  
Single Chain ◽  
Nih3t3 Cell Line ◽  
Velvet Antler ◽  
Velvet Antler Polypeptide

A novel polypeptide, velvet antler polypeptide (VAPPs), having a stimulary effect on proliferation of some cell was isolated from the velvet antler of sika deer (Cervus nippon Temminck). This polypeptide consists of a single chain of 32 amino-acid residues VLSAT DKTNV LAAWG KVGGN APAFG AEALE RM. VAPPs showed marked stimulary effect on rat epidermal cells and NIH3T3 cell line (dose range from 10-40 mg x L(-1) and 5-80 mg x L(-1), respectively).

scholarly journals Establishment and Application of Engineered NIH 3T3 Cell Line with Stable Human RAGE Expression

2020 ◽  
Vol 02 (01) ◽  
pp. e23-e27
Author(s):  
Xinyi Xiao ◽  
Hui Chen ◽  
Pameila Paerhati ◽  
Meiqi Zhou ◽  
Zhuoyi Yang ◽  
...  
Keyword(s):  
Cell Line ◽  
Molecular Mechanisms ◽  
Amyloid Β ◽  
Cellular Level ◽  
Nih3t3 Cell ◽  
Stable Cell Line ◽  
Nih3t3 Cells ◽  
Nih3t3 Cell Line ◽  
Lentivirus Transduction ◽  
Rage Expression

Abstract Aim NIH3T3 cell line with expression of human receptor for advanced glycation end-products (hRAGE) transduced with lentivirus vectors was used to analyze affinity, biological activity, and/or molecular mechanisms of molecules targeting the hRAGE pathway. Method The DNA fragment coding for hRAGE gene was integrated into the genome of NIH3T3 cells using lentivirus transduction. Cells expressing hRAGE were selected with puromycin, and the level of hRAGE expression was analyzed by Western blot. To establish a stable cell line, colonies of hRAGE-expressing cells were generated, and the level of RAGE expression in each engineered cell line was analyzed within 20 generations. Flow cytometry assay was used to verify affinity of anti-hRAGE antibody binding to hRAGE on the surface of engineered cells. The engineered NIH3T3 cell line was applied to assess effects of anti-hRAGE blocking antibody on amyloid β-induced cells apoptosis by CCK-8 assay. Results The engineered NIH3T3 cell line (hRAGE-NIH3T3) could stably express human RAGE. Commercial anti-RAGE polyclonal antibody could recognize and bind to human RAGE on the surface of hRAGE-NIH3T3 but not original NIH3T3 cells. In addition, hRAGE-NIH3T3 was more sensitive to RAGE pathway-dependent stimulation. Our data show that the hRAGE-NIH3T3 cell line established is an excellent tool in the study of RAGE-targeting molecules based on the cellular level, biological function, and RAGE-mediated molecular mechanisms.

scholarly journals CYTOTOXIC EFFECTS OF ARIPIPRAZOLE ON MKN45 AND NIH3T3 CELL LINES AND GENOTOXIC EFFECTS ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES

2019 ◽  
Vol 56 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Mohammad SHOKRZADEH ◽  
Abbas MOHAMMADPOUR ◽  
Mona MODANLOO ◽  
Melika HASSANI ◽  
Nasrin Ghassemi BARGHI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Normal Cell ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Nih3t3 Cell ◽  
Dopamine Antagonists ◽  
Blood Lymphocytes

ABSTRACT BACKGROUND: Gastric cancer is known as the fourth most common cancer. Current treatments for cancer have damaged the sensitive tissues of the healthy body, and in many cases, cancer will be recurrent. Therefore, need for treatments that are more effective is well felt. Researchers have recently shifted their attention towards antipsychotic dopamine antagonists to treat cancer. The anticancer activities of aripiprazole remain unknown. OBJECTIVE: This study aimed to evaluate the efficacy and safety of aripiprazole on gastric cancer and normal cell lines. METHODS: In this regard, the cytotoxicity and genotoxicity of aripiprazole were investigated in MKN45 and NIH3T3 cell lines by methyl tetrazolium assay and on peripheral blood lymphocytes by micronucleus assay. For this purpose, cells were cultured in 96 wells plate. Stock solutions of aripiprazole and cisplatin were prepared. After cell incubation with different concentrations of aripiprazole (1, 10, 25, 50, 100 and 200 μL), methyl tetrazolium solution was added. For micronucleus assay fresh blood was added to RPMI culture medium 1640 supplemented, and different concentrations of aripiprazole (50, 100 and 200 μL) were added. RESULTS: The finding of present study showed that the IC50 of aripiprazole in the cancer cell line (21.36 μg/mL) was lower than that in the normal cell line (54.17 μg/mL). Moreover, the micronucleus assay showed that the frequency of micronuclei of aripiprazole at concentrations below 200 μM was much less than cisplatin. CONCLUSION: Aripiprazole can be a good cytotoxic compound and good candidate for further studies of cancer therapy.

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