scholarly journals Comparison of Anticancer Effects of Hydroalcoholic Extracts of Camellia sinensis and Lepidium sativum L on HeLa Cell Line

2020 ◽  
Vol 13 (11) ◽  
Author(s):  
Somayeh Jahani ◽  
Zahra Heidari ◽  
Mehdi Azami ◽  
Bita Moudi
Keyword(s):  
Hela Cell ◽  
Growth Inhibition ◽  
Cell Line ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Hela Cells ◽  
Green Tea Extract ◽  
Lepidium Sativum ◽  
Hela Cell Line ◽  
Tea Extract

Background: The antioxidative activity of green tea and garden cress extract is of interest in cancer. Objectives: The current study aimed at evaluating the effect of hydroalcoholic extracts of Lepidium sativum (cress) and Camellia sinensis (green tea) on the culture medium of the HeLa cell line. Methods: Dulbecco’s Modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) was used to culture HeLa cells, which was exposed to the different concentrations of green tea and cress extracts at 24 hours and 48 hours. Cell viability and apoptotic cells were quantified by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT assay and propidium iodide, respectively. Results: The highest percentage of growth inhibition (85%) was observed at 100 μg/mL of the green tea extract after 48-hour treatment. The percentage of growth inhibition at 24 h after treatment was 83% for green tea (P > 0.05). The high growth inhibition percentage of HeLa cells at 100 μg/mL of cress extract at 24 hours and 48 hours (49.8%) after treatment was 27.92% and 49.8%, respectively (P > 0.05). Additionally, the cell apoptosis assay indicated that green tea and cress extracts had toxic effects on the HeLa cells. This effect was highest at the concentration of 100 μg/mL and more evident in green tea. Conclusions: It can be concluded that green tea extract compared with cress had a more cytotoxic effect against cervical cancer.

scholarly journals Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In Vitro

2011 ◽  
Vol 62 (2) ◽  
pp. 139-146 ◽  
Author(s):  
Ksenija Durgo ◽  
Sandra Kostić ◽  
Katarina Gradiški ◽  
Draženka Komes ◽  
Maja Osmak ◽  
...  
Keyword(s):  
Cell Line ◽  
Green Tea ◽  
Laryngeal Carcinoma ◽  
Resistant Cell ◽  
Resistant Cell Line ◽  
Green Tea Extract ◽  
Carcinoma Cells ◽  
Tea Extract ◽  
Human Laryngeal Carcinoma

Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In VitroGreen tea (Camellia sinensis) contains several bioactive compounds which protect the cell and prevent tumour development. Phytochemicals in green tea extract (mostly flavonoids) scavenge free radicals, but also induce pro-oxidative reactions in the cell. In this study, we evaluated the potential cytotoxic and prooxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) on human laryngeal carcinoma cell line (HEp2) and its cross-resistant cell line CK2. The aim was to see if the extract and its two flavonoids could increase the sensitivity of the cisplatin-resistant cell line CK2 in comparison to the parental cell line. The results show that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. The pro-oxidant effect of green tea was determined at concentrations higher than those found in traditionally prepared green tea infusions.

scholarly journals Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis)

2007 ◽  
Vol 19 (1) ◽  
Author(s):  
Isti Endah Kurniwati ◽  
Juni Handajani ◽  
Regina TC. Tandelilin
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Dental Plaque ◽  
Health Benefit ◽  
Green Tea Extract ◽  
Control Group ◽  
Research Subjects ◽  
Significant Difference ◽  
Tea Extract

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.

scholarly journals Effect of camellia sinensis extract on the expression level of transcription factors and cytochrome p450 genes coding phase i drug-metabolizing enzymes

2013 ◽  
Vol 59 (4) ◽  
pp. 45-59
Author(s):  
Anna Bogacz ◽  
Monika Karasiewicz ◽  
Joanna Bartkowiak-Wieczorek ◽  
Marcin Ożarowski ◽  
Agnieszka Seremak-Mrozikiewicz ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Mrna Level ◽  
Green Tea Extract ◽  
Expression Level ◽  
Gene Transcript ◽  
Mrna Levels ◽  
Clinically Significant ◽  
Tea Extract

Abstract Green tea (Camellia sinensis) is widely used as a popular beverage and dietary supplement that can significantly reduce the risk of many diseases. Despite the widespread use of green tea, the data regarding the safety as well as herb-drug interactions are limited. Therefore, the aim of our study was to assess the influence of standardized green tea extract (GTE) containing 61% catechins and 0.1% caffeine on the expression level of rat CYP genes and the corresponding transcription factors expression by realtime PCR. The findings showed that GTE resulted in a significant decrease of CYP2C6 expression level by 68% (p<0.001). In case of CYP3A1 and CYP3A2, the mRNA levels were also reduced by extract but in a lesser degree compared to CYP2C6. Simultaneously the significant increase in the mRNA level of CAR, RXR and GR factors was observed by 54% (p<0.05), 79% (p<0.001) and 23% (p<0.05), respectively after 10 days of green tea extract administration. In addition, there was noted a small increase of CYP1A1 expression level by 21% (p>0.05) was noted. No statistically significant differences were observed for CYP1A2 and CYP2D1/2. In the same study we observed an increase in amount of ARNT gene transcript by 27% (p<0.05) in the long-term use. However, green tea extract showed the ability to stimulate HNF-1α both after 3 and 10 days of treatment by 30% (p<0.05) and 80% (p<0.001), respectively. In contrast, no change was observed in the concentration of HNF-4α cDNA. These results suggest that GTE may change the expression of CYP enzymes, especially CYP2C6 (homologue to human CYP2C9) and may participate in clinically significant interactions with drugs metabolized by these enzymes.

scholarly journals Effect of green tea extract (Camellia sinensis) on the spermatic parameters of Wistar rats submitted or not to testicular heat shock

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Joane Isis Travassos Vieira ◽  
Taylane Alves da Silva ◽  
Williane Maria Pereira Barbosa ◽  
Gabriela Lima de Azevêdo ◽  
Lúcia Cristina Pereira Arruda ◽  
...  
Keyword(s):  
Heat Shock ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Wistar Rats ◽  
Green Tea Extract ◽  
Tea Extract

scholarly journals In vitro Anticancer Activity Effect of Extracellular Metabolites of Some Bacterial Species on HeLa Cell Line.

2020 ◽  
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Line ◽  
Hela Cells ◽  
Bacterial Species ◽  
Hela Cell Line ◽  
Inhibitory Effects ◽  
Anticancer Effect ◽  
E Coli ◽  
Extracellular Metabolites

Background: Cancer is still one of the most serious problems that affect human health. Despite the intense efforts to develop treatments, effective agents are still not available. In some cases, conventional therapy could be harmful or fail because of emerging drug resistance. Therefore, the development of novel therapies against cancer is of utmost importance. Assessment of anticancer effects of bacterial metabolites on cancer cells may help in the process of finding new cheap, reliable, contentious and safe cancer therapy. Objective: To determine the anticancer effect of the extracellular metabolites of eight bacterial species on HeLa cell line. Methodology: Extracellular metabolites were prepared by isolating and culturing eight bacterial species (Escherichia coli, Staphylococcus aureus, Micrococcus, Pseudomonas aeruginosa, Lactic acid bacteria, Klebsiella, Proteus and E. coli with its phage) in liquid media. Tubes were incubated overnight and centrifuged. Supernatant was filtered and concentrated using Infra-Red concentrator. Different concentrations were prepared and their anticancer effect were evaluated using MTT cell proliferation assay. Results: Results showed variation among the eight bacteria concerning proliferation inhibition against HeLa cells in a time and concentration dependent manner. Pseudomonas and E. coli with its phage revealed considerable anticancer activity with 63% and 86% inhibitory effects (both at 1000 µg\ml) and IC50 of 301 and 1395 µg/dl at 24h respectively. While Proteus and Micrococcus showed low inhibitory effects and S. aureus enhanced the proliferation of HeLa cells at low concentrations. Conclusion: Among the tested bacteria, Pseudomonas and E. coli and its phage gave the best anticancer inhibitory effects against HeLa cells. Further studies using purified components of effective bacteria are recommended.

scholarly journals Investigation of the Dose-Enhancement Effects of Spherical and Rod-Shaped Gold Nanoparticles on the HeLa Cell Line

2020 ◽  
Vol 9 ◽  
pp. 1581
Author(s):  
Samad Amani ◽  
Alireza Mehdizadeh ◽  
Mohammad Mehdi Movahedi ◽  
Marzieh Keshavarz ◽  
Fereshteh Koosha
Keyword(s):  
Gold Nanoparticles ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cells ◽  
Current Treatment ◽  
X Rays ◽  
Hela Cell Line ◽  
X Ray ◽  
Cervical Cancer Cells ◽  
Gold Nanomaterials

Background: Cervical cancer cells are known as radioresistant cells. Current treatment methods have not improved the patients’ survival efficiently; thus, new therapeutic strategies are needed to enhance the efficacy of radiotherapy. Gold nanomaterials with different shapes and sizes have been explored as radiosensitizers. The present study compared the radiosensitizing effects of gold nanorods (AuNRs) with spherical gold nanoparticles (AuNPs) on the HeLa cell line irradiated with megavoltage X-rays. Materials and Methods: The cytotoxicity of AuNRs and AuNPs on HeLa cells in the presence and absence of 6-MV X-ray was investigated using the MTT assay. For this aim, HeLa cells were incubated with and AuNPs and AuNRs at various concentrations (5, 10, and 15 µg/mL) for 6 hours. Afterward, HeLa cells were irradiated with 6-MV X-ray at a single dose of 2 Gy. Results: The results showed that the addition of AuNRs and AuNPs could enhance the radiosensitivity of HeLa cells. Both AuNRs and AuNPs showed low toxicity on HeLa cells, while AuNRs were more toxic than AuNPs at the examined concentrations. Moreover, it was found that AuNRs could enhance the radiosensitivity of HeLa cells more than spherical-shaped AuNPs. Conclusion: This study revealed that the shape of nanoparticles is an effective factor when they are used as radiosensitizing agents during radiotherapy. [GMJ.2020;9:e1581]

scholarly journals PENGARUH PENGGUNAAN EKSTRAK DAUN TEH HIJAU (Camellia sinensis) PADA PROSES PEMBUATAN NAGET DAGING SAPI TERHADAP SIFAT KIMIA

2020 ◽  
Vol 1 (1) ◽  
pp. 34
Author(s):  
Muhammad Fakhri Maulana ◽  
Lilis Suryaningsih ◽  
Andry Pratama
Keyword(s):  
Moisture Content ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Design Method ◽  
Chemical Properties ◽  
Fat Content ◽  
Green Tea Extract ◽  
Polyphenol Content ◽  
Randomized Design ◽  
Tea Extract

Green tea is known as a plant who has many purpose as well as an antioxidant sources. The purpose of this research is to find out the effect of adding green tea (Camellia sinensis) extract in beef nuggets on chemical properties (moisture content, fat content, and polyphenol content). This research is an experimental research using completely randomized design method with 4 treatments and 5 repetition. The treatment that given was P1= beef nugget with no added green tea extract, P2= beef nugget + 0,5% green tea extract, P3= beef nugget + 1% green tea extract, and P4= beef nugget + 1,5% green tea extract with 5 repetition. Chemical analysis that used in this research is moisture content analysis, fat content, and polyphenols content. The obtained results then tested with anova, duncan, and polynomial test. The results showed that the treatment had a significantly real effect on polyphenols content but not on the moisture content and fat content. The addition of 1,5% green tea (Camellia sinensis) extract increase the polyphenol content of 305,96 mg/kg and influence 80,79% of the polyphenol content.

scholarly journals Cytotoxic activity of Pinus cembra L. needle extract: A preliminary study on HeLa cell line

2021 ◽  
Vol 57 (2) ◽  
pp. 93-98
Author(s):  
Cristina LUNGU ◽  
◽  
Cosmin-Teodor MIHAI ◽  
Gabriela VOCHITA ◽  
Daniela GHERGHEL ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cells ◽  
Pine Needles ◽  
Hela Cell Line ◽  
Pine Needle ◽  
Cytotoxic Effects ◽  
Antitumor Potential ◽  
Cembran Pine

The aim of this study was to investigate the cytotoxic effects of a hydromethanolic extract obtained from cembran pine needles in HeLa cell line. In this respect, the effects of needle extract on protein synthesis, viability, proliferation and cell cycle in HeLa cells were evaluated after 48 h treatment. Cembran pine needle extract dose-dependently decreased protein synthesis in HeLa cells causing 44.26% reduction in protein synthesis at 100µg/ml. At 25, 50 and 100µg/ml, it increased cell death in comparison with the control (20.99%, 21.49% and 23.63%, respectively vs. 9.83%). In addition, at 100µg/ml, cembran pine needle extract showed a remarkable antiproliferative effect whereas at 25 and 50µg/ml, it induced sub-G1 phase cells accumulation (11.68 ± 0.81% and 14.69 ± 0.56%, respectively in comparison with control, 6.03 ± 0.55%), an indicator of proapoptotic effects. Taken together, these results indicate that cembran pine needles are a source of compounds with antitumor potential which needs to be further investigated and exploited.

scholarly journals Developing a Low-fat Drinking Yoghurt by Incorporating Green Tea (Camellia sinensis) Extract as a Functional Ingredient

2021 ◽  
Author(s):  
R.A.A.S. Ranasinghe ◽  
M.P. Edirisinghe ◽  
W.A.D. Nayananjalie
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Titratable Acidity ◽  
Green Tea Extract ◽  
Organoleptic Properties ◽  
Sensory Data ◽  
Green Tea Leaves ◽  
Microbial Analysis ◽  
Tea Extract ◽  
Sterilized Milk

Background: Nowadays people are health-concerned and consume more of functional foods. Thus, this study is focused on developing a drinking yoghurt incorporated with green tea (Camellia sinensis) extract as a functional ingredient.Methods: Green tea extract (GTE) was obtained by brewing 02 g of dried green tea leaves in 100 mL of water at 90°C for 30 minutes. Drinking yoghurts were prepared with standardized sterilized milk incorporated with 0%, 10%, 15%, 20% and 25% (v/v) of GTE. Physio-chemical, microbial and sensory analysis were conducted to evaluate the quality attributes of the developed product. It was stored under refrigerated conditions (4°C) for 15 days.Result: Sensory data showed that 25% green tea extract incorporated drinking yoghurt had the best organoleptic properties. It had the significantly (p less than 0.05) highest ash and lowest fat contents compared to the control. Significantly (p less than 0.05) higher pH and lower titratable acidity values were observed in 25% green tea extract incorporated yoghurt compared to the control on 15th day of storage. The microbial analysis did not show the presence of coliform. In conclusion, yoghurt incorporated with 25% (v/v) green tea extract has better organoleptic properties and nutritional value while it can be stored under refrigerated conditions for 15 days without any quality deterioration while producing at affordable price.

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