scholarly journals Comparative Proteomic Analysis of Breast Cancer Tissue and the Adjacent Normal Tissue in Iranian Patients with HER2 Negative Ductal Carcinoma of Breast

2017 ◽  
Vol 10 (5) ◽  
Author(s):  
Mayram Amiri-Shoar ◽  
Masoumeh Hosseini ◽  
Mitra Arianmanesh ◽  
Reza Eghdam-Zamiri ◽  
Shahrokh Attarian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Proteomic Analysis ◽  
Normal Tissue ◽  
Ductal Carcinoma ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Adjacent Normal Tissue ◽  
Comparative Proteomic Analysis ◽  
Iranian Patients

Expressions of miR-21 and miR-210 in Breast Cancer and Their Predictive Values for Prognosis

2020 ◽  
Author(s):  
Xiaofei WU
Keyword(s):  
Breast Cancer ◽  
Normal Tissue ◽  
Breast Cancer Tissue ◽  
Clinical Staging ◽  
Cancer Tissue ◽  
Adjacent Normal Tissue ◽  
Normal Tissues ◽  
Qrt Pcr ◽  
Node Metastasis ◽  
Low Expression

Background: We aimed to investigate the expressions of miR-21 and miR-210 in the breast cancer tissue and their correlation with clinicopathological features and prognosis. Methods: A retrospective analysis was performed on 68 patients with breast cancer treated surgically in Wuhan General Hospital of Guangzhou Military in 2014-2015. The breast cancer tissue and the adjacent normal tissue were collected from the patients. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of miR-21 and miR-210 in the breast cancer and adjacent normal tissues. Results: According to qRT-PCR, the expression levels of miR-210 and miR-21 in the breast cancer tissue were significantly higher than those in the adjacent normal tissue (P<0.05), which were significantly correlated with lymph node metastasis, clinical staging and differentiation of patients (P<0.05). miR-21 and miR-210 were significantly positive correlated in both breast cancer tissues and adjacent normal tissues (r=0.7014, 0.7502, P<0.001). The survival rate in the miR-210 high expression group was significantly lower than that in the miR210 low expression group (P<0.05), whereas there was no significant difference between the miR-21 high and low expression groups. Conclusion: miR-21 and miR-210 are highly expressed in the breast cancer tissue and significantly correlated with lymph node metastasis, clinical staging and differentiation. miR-210, the up-regulated expression of which is related to the poor prognosis of patients with breast cancer, may be a potential prognostic indicator for breast cancer, which can be used to judge the prognosis.

Membrane proteomic analysis comparing squamous cell lung cancer tissue and tumour-adjacent normal tissue

2012 ◽  
Vol 319 (1) ◽  
pp. 118-124 ◽  
Author(s):  
Burong Li ◽  
Jiantong Chang ◽  
Yonglie Chu ◽  
Huafeng Kang ◽  
Jun Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Squamous Cell ◽  
Cell Lung Cancer ◽  
Proteomic Analysis ◽  
Normal Tissue ◽  
Squamous Cell Lung Cancer ◽  
Cancer Tissue ◽  
Adjacent Normal Tissue ◽  
Lung Cancer Tissue

Breast cancer in Irish families with Lynch syndrome.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 1543-1543
Author(s):  
Emmet Jordan ◽  
Michael P. Farrell ◽  
Karen Anne Cadoo ◽  
R. M. Clarke ◽  
C. Nolan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prostate Cancer ◽  
Colorectal Cancer ◽  
Lynch Syndrome ◽  
Hormone Receptor ◽  
Ductal Carcinoma ◽  
Hormone Receptor Status ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Receptor Status

1543 Background: Breast cancer is not among the recognised malignant manifestations of Lynch Syndrome which include colorectal, endometrial, gastric, ovarian and upper urinary tract tumours. In this study we report the prevalence of breast cancer in Irish Lynch Syndrome families and determine immunohistochemical (IHC) expression of mismatch repair proteins (MMR) in available breast cancer tissue. Methods: Breast cancer prevalence was determined among Lynch Syndrome kindreds from two institutions in Ireland. One kindred was omitted due to a biallelic MMR and BRCA1 mutation.The clinicopathological data that was collected on breast cancer cases included age of onset, morphology, and hormone receptor status, and a genotype phenotype correlation was investigated. Immunohistochemical staining was performed for MLH1, MSH2, MSH6, and PMS2 on all available breast cancer tissue from affected individuals. Results: The distribution of MMR mutations seen in sixteen pedigrees were as follows; MLH1 (n=5), MSH2 (7), MSH6 (3), PMS2 (1). Sixty cases of colorectal cancer and 14 cases of endometrial cancer were seen. Seven breast cancers (5 invasive ductal and 2 invasive lobular cancers) and 1 case of ductal carcinoma in situ were reported in 7 pedigrees. This compared with 4 cases of prostate cancer. Of the 7 LS kindreds containing breast cancer, 6 MSH2 mutations and 1 MSH6 mutations were identified. Median age of breast cancer diagnosis was 49 years (range 38-57). Hormone receptor status is available on 3 breast cancer cases at time of abstract submission; all were ER positive and HER 2 negative. All cases had grade 2 or 3 tumours. 5 samples were available for IHC evaluation. 3 out of 5 cases showed loss of MMR expression, all showed loss of MSH2 and MSH6 expression. One of the two cases with normal IHC expression in breast tissue belonged to a kindred where 3 siblings with colorectal cancer and documented deleterious mutations demonstrated no IHC loss. Conclusions: Breast cancer occurred at an early age and was more common than prostate cancer in Irish Lynch Syndrome pedigrees. All identified breast cancer were in kindreds with MSH2 or MSH6 mutations. Enhanced breast cancer screening may be warranted in certain Lynch Syndrome kindreds.

scholarly journals Proteomic analysis of breast cancer tissue reveals upregulation of actin-remodeling proteins and its relevance to cancer invasiveness

2008 ◽  
Vol 3 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Dong-Hyun Kim ◽  
Jinhee Bae ◽  
Jong Won Lee ◽  
Seon-Young Kim ◽  
Yong-Hak Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Proteomic Analysis ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Actin Remodeling ◽  
Cancer Invasiveness

scholarly journals Indication of high lipid content in epithelial-mesenchymal transitions of breast tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Siti Norbaini Sabtu ◽  
S. F. Abdul Sani ◽  
L. M. Looi ◽  
S. F. Chiew ◽  
Dharini Pathmanathan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Multivariate Analysis ◽  
Nucleic Acids ◽  
Raman Spectra ◽  
Cancer Progression ◽  
Component Analysis ◽  
Breast Cancer Tissue ◽  
Metabolic Changes ◽  
Cancer Tissue

AbstractThe epithelial-mesenchymal transition (EMT) is a crucial process in cancer progression and metastasis. Study of metabolic changes during the EMT process is important in seeking to understand the biochemical changes associated with cancer progression, not least in scoping for therapeutic strategies aimed at targeting EMT. Due to the potential for high sensitivity and specificity, Raman spectroscopy was used here to study the metabolic changes associated with EMT in human breast cancer tissue. For Raman spectroscopy measurements, tissue from 23 patients were collected, comprising non-lesional, EMT and non-EMT formalin-fixed and paraffin embedded breast cancer samples. Analysis was made in the fingerprint Raman spectra region (600–1800 cm−1) best associated with cancer progression biochemical changes in lipid, protein and nucleic acids. The ANOVA test followed by the Tukey’s multiple comparisons test were conducted to see if there existed differences between non-lesional, EMT and non-EMT breast tissue for Raman spectroscopy measurements. Results revealed that significant differences were evident in terms of intensity between the non-lesional and EMT samples, as well as the EMT and non-EMT samples. Multivariate analysis involving independent component analysis, Principal component analysis and non-negative least square were used to analyse the Raman spectra data. The results show significant differences between EMT and non-EMT cancers in lipid, protein, and nucleic acids. This study demonstrated the capability of Raman spectroscopy supported by multivariate analysis in analysing metabolic changes in EMT breast cancer tissue.

Quantitative estimation of BRCA1 protein expression in breast cancer tissue using the method of flow cytometry

2016 ◽  
Vol 61 ◽  
pp. S183
Author(s):  
E. Shestakova ◽  
E. Dudko ◽  
A. Grishanina ◽  
V. Kirsanov ◽  
N. Vichljantzeva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Protein Expression ◽  
Quantitative Estimation ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Brca1 Protein

Detection of DNA fragmentation in human breast cancer tissue by an antibody specific to single-stranded DNA

Breast Cancer ◽  
1998 ◽  
Vol 5 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Shunzo Kobayashi ◽  
Hirotaka Iwase ◽  
Yoshihiko Kawarada ◽  
Naoyuki Miura ◽  
Toshihiro Sugiyama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Fragmentation ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Single Stranded Dna
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