scholarly journals Co-Administration of Anti-Angiogenic Peptide and DNA Vaccine in Cervical Cancer Tumor Model

2017 ◽  
Vol 10 (3) ◽  
Author(s):  
Parisa Saadat ◽  
Hoorieh Soleimanjahi ◽  
Seyed Mohsen Asghari ◽  
Maryam Fazeli ◽  
Hadi Razavinikoo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Vaccine ◽  
Tumor Model ◽  
Cancer Tumor

scholarly journals Circ-CCDC66 Upregulates REXO1 Expression to Aggravate Cervical Cancer Progression Via Restraining miR-452-5p

2020 ◽  
Author(s):  
Yun Zhang ◽  
Xing Li ◽  
Jun Zhang ◽  
Lin Mao
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Cancer Progression ◽  
Tumor Model ◽  
Tumor Stage ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Cell Fractionation ◽  
Real Time Quantitative Pcr ◽  
Cancer Tumor

Abstract BackgroundCervical cancer is one most common cancer types among females around the world. CircRNAs have been revealed to participate in multiple biological functions, and are involved in many diseases’ progression. In current study, we aimed to elucidate whether circ-CCDC66 participates cervical cancer progression. MethodsReal-time quantitative PCR (RT-qPCR) was conducted to measure the expression of circ-CCDC66, miR-452-5p, and REXO1 mRNA. Cell fractionation assay and RNA fluorescence in situ hybridization (FISH) were performed to locate circ-CCDC66 in cells. Cell proliferation ability was detected using cell account kit 8 (CCK-8). TRANSWELL assay was applied to evaluate cell migration or invasion ability. Bioinformatics analysis, biotinylated RNA pull-down, RNA immunoprecipitation, and dual-luciferase reporter assays were conducted to assess the association between miR-452 and circ-CCDC66 or REXO1. Western blot was applied to measure the protein expression of REXO1. Animal tumor model was used to assess the effect of circ-CCDC66 in vivo.ResultsCirc-CCDC66 was upregulated in cervical cancer tumor tissues, and correlated with tumor stage and tumor size. Downregulation of circ-CCDC66 inhibited cervical cancer cell proliferation, migration, and invasion. Circ-CCDC66 was an efficient molecular sponge for miR-452-5p, and negatively regulated miR-452-5p expression. MiR-452 directly targeted REXO1. The effects of circ-CCDC66 on cervical cancer cells were functioned through miR-452-5p/REXO1 axis. In animal experiment, downregulation of circ-CCDC66 was found to suppress tumor growth in vivo.ConclusionOur results demonstrated the effects of circ-CCDC66/miR-452-5p/REXO1 axis in cervical cancer progression, we might provide a novel therapeutic target for cervical cancer.

Long Non-Coding RNA ARAP1-AS1 Facilitates the Progression of Cervical Cancer by Regulating miR-149-3p and POU2F2

Pathobiology ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ling Zhou ◽  
Xiao-li Xu
Keyword(s):  
Cervical Cancer ◽  
Tumor Model ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Non Coding Rna ◽  
Injection Model ◽  
Rna Immunoprecipitation ◽  
Long Non Coding Rna

<b><i>Background:</i></b> Emerging research has demonstrated that long non-coding RNAs (lncRNAs) attach great importance to the progression of cervical cancer (CC). LncRNA ARAP1-AS1 was involved in the development of several cancers; however, its role in CC is far from being elucidated. <b><i>Methods:</i></b> Real-time PCR (RT-PCR) was employed to detect ARAP1-AS1 and miR-149-3p expression in CC samples. CC cell lines (HeLa and C33A cells) were regarded as the cell models. The biological effect of ARAP1-AS1 on cancer cells was measured using CCK-8 assay, colony formation assay, flow cytometry, Transwell assay and wound healing assay in vitro, and subcutaneous xenotransplanted tumor model and tail vein injection model in vivo. Furthermore, interactions between ARAP1-AS1 and miR-149-3p, miR-149-3p and POU class 2 homeobox 2 (POU2F2) were determined by bioinformatics analysis, qRT-PCR, Western blot, luciferase reporter and RNA immunoprecipitation assay, respectively. <b><i>Results:</i></b> The expression of ARAP1-AS1 was enhanced in CC samples, while miR-149-3p was markedly suppressed. Additionally, ARAP1-AS1 overexpression enhanced the viability, migration, and invasion of CC cells. ARAP1-AS1 downregulated miR-149-3p via sponging it. ARAP1-AS1 and miR-149-3p exhibited a negative correlation in CC samples. On the other hand, ARAP1-AS1 enhanced the expression of POU2F2, which was validated as a target gene of miR-149-3p. <b><i>Conclusion:</i></b> ARAP1-AS1 was abnormally upregulated in CC tissues and indirectly modulated the POU2F2 expression via reducing miR-149-3p expression. Our study identified a novel axis, ARAP1-AS1/miR-149-3p/POU2F2, in CC tumorigenesis.

Early-stage cervical cancer: Tumor delineation by magnetic resonance imaging and ultrasound — A European multicenter trial

2013 ◽  
Vol 128 (3) ◽  
pp. 449-453 ◽  
Author(s):  
Elisabeth Epstein ◽  
Antonia Testa ◽  
Adrius Gaurilcikas ◽  
Alessia Di Legge ◽  
Liveke Ameye ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Cervical Cancer ◽  
Magnetic Resonance ◽  
Early Stage ◽  
Multicenter Trial ◽  
Resonance Imaging ◽  
Tumor Delineation ◽  
Early Stage Cervical Cancer ◽  
Cancer Tumor

scholarly journals Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis

2021 ◽  
Vol 12 (10) ◽  
Author(s):  
Chunyu Zhang ◽  
Pan Liu ◽  
Jiaming Huang ◽  
Yuandong Liao ◽  
Chaoyun Pan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Tumor Growth ◽  
Poor Prognosis ◽  
Prognostic Biomarker ◽  
Circular Rna ◽  
Circular Rnas ◽  
Inhibit Tumor Growth ◽  
Cancer Tumor ◽  
Tumor Growth And Metastasis

AbstractCircular RNAs (circRNAs) are known to act as key regulators in a variety of malignancies. However, the role of circRNAs in cervical cancer (CCa) remains largely unknown. Herein, we demonstrated that a circRNA derived from the TADA2A gene (hsa_circ_0043280) was significantly downregulated in CCa and that this reduction in expression was correlated with a poor prognosis. Furthermore, our results demonstrated that hsa_circ_0043280 functions as a tumor suppressor to inhibit tumor growth and metastasis in CCa. Mechanistically, hsa_circ_0043280 competitively sponges miR-203a-3p and prevents miR-203a-3p from reducing the levels of PAQR3. Collectively, our results demonstrate that hsa_circ_0043280 plays a pivotal role in the development and metastasis of CCa, thus suggesting that hsa_circ_0043280 has significant potential as a prognostic biomarker and a therapeutic target for CCa.

PMMA particle-mediated DNA vaccine for cervical cancer

2009 ◽  
Vol 88A (4) ◽  
pp. 849-857 ◽  
Author(s):  
Pei-Jen Lou ◽  
Wen-Fang Cheng ◽  
Yi-Chen Chung ◽  
Che-Yuan Cheng ◽  
Lien-Hua Chiu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Vaccine

A 700-kb physical and transcription map of the cervical cancer tumor suppressor gene locus on chromosome 11q13

Genomics ◽  
2005 ◽  
Vol 85 (6) ◽  
pp. 704-714 ◽  
Author(s):  
Kayvan Zainabadi ◽  
Payam Benyamini ◽  
Rita Chakrabarti ◽  
Mysore S. Veena ◽  
Settara C. Chandrasekharappa ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Gene Locus ◽  
Suppressor Gene ◽  
Chromosome 11Q13 ◽  
Cancer Tumor ◽  
Transcription Map

Alternatively spliced human tissue factor promotes tumor growth and angiogenesis in a pancreatic cancer tumor model

2007 ◽  
Vol 120 ◽  
pp. S13-S21 ◽  
Author(s):  
Jennifer E. Hobbs ◽  
Anaadriana Zakarija ◽  
Deborah L. Cundiff ◽  
Jennifer A. Doll ◽  
Emily Hymen ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Tumor Growth ◽  
Tissue Factor ◽  
Human Tissue ◽  
Tumor Model ◽  
Cancer Tumor ◽  
Alternatively Spliced

scholarly journals Sonophoresis Enhanced Transdermal Delivery of Cisplatin in the Xenografted Tumor Model of Cervical Cancer

2020 ◽  
Vol Volume 13 ◽  
pp. 889-902
Author(s):  
Shanshan Ma ◽  
Chang Liu ◽  
Bo Li ◽  
Tingting Zhang ◽  
Li Jiang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Transdermal Delivery ◽  
Tumor Model ◽  
Xenografted Tumor

scholarly journals The antitumor efficiency of combined electrochemotherapy and single dose irradiation on a breast cancer tumor model

2012 ◽  
Vol 46 (3) ◽  
Author(s):  
Elham Raeisi ◽  
Seyed Aghamiri ◽  
Azin Bandi ◽  
Negar Rahmatpour ◽  
Seyed Firoozabadi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Single Dose ◽  
Tumor Model ◽  
Dose Irradiation ◽  
Cancer Tumor

scholarly journals Prospective intra/inter-observer evaluation of pre-brachytherapy cervical cancer tumor width measured in TRUS and MR imaging

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Mario Federico ◽  
Carmen Rosa Hernandez-Socorro ◽  
Ivone Ribeiro ◽  
Jesus Gonzalez Martin ◽  
Maria Dolores Rey-Baltar Oramas ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Patients ◽  
Clinical Information ◽  
Figo Stage ◽  
Quantitative Agreement ◽  
Stage I ◽  
Maximum Width ◽  
Dose Adaptation ◽  
Wide Availability ◽  
Cancer Tumor

Abstract Background Ultrasound (US) imaging has been proved as an excellent diagnostic tool in gynecology and, due to its wide availability and limited cost, is under intense investigation as base for dose adaptation in cervical cancer brachytherapy. Purpose of this work is to test inter/intra-observer uncertainties between magnetic resonance (MR) and trans-rectal ultrasound (TRUS) imaging in defining maximum tumor width before first brachytherapy (BT) application in a prospective cohort of cervical cancer patients undergoing image-guided adaptive brachytherapy (IGABT). Methods One hundred ten consecutive cervical cancer patients treated between 2013 and 2016 were included. Before the first BT implant patients underwent MR and TRUS scan with no applicator in place. Images were independently analyzed by three examiners, blinded to the other’s results. With clinical information at hand, maximum tumor width was measured on preBT TRUS and MR. Quantitative agreement analysis was undertaken. Intra-class correlation coefficient (ICC), Passing-Bablok and Bland Altman plots were used to evaluate the intra/inter-observers measurement agreement. Results Average difference between tumor width measured on MR (HRCTVMR) and TRUS (HRCTVTRUS) was 1.3 ± 3.2 mm (p <  0.001); 1.1 ± 4.6 mm (p = 0.01) and 0.7 ± 3 mm (p = 0.01). The error was less than 3 mm in 79, 82 and 80% of the measurements for the three observers, respectively. Intra-observer ICC was 0.96 (CI95% 0.94–0.97), 0.93 (CI95% 0.9–0.95) and 0.96 (CI95% 0.95–0.98) respectively. Inter-observer ICC for HRCTVMR width measures was 0.92 (CI95% 0.89–0.94) with no difference among FIGO stages. Inter-observer ICC for HRCTVTRUS was 0.86 (CI95% 0.81–0.9). For FIGO stage I and II tumors, ICC HRCTVTRUS values were comparable to respective HRCTVMR ICC values. For larger tumors HRCTVTRUS inter-observer ICC values were lower than respective HRCTVMR although remaining acceptable. Conclusions Our results suggest that TRUS is equivalent to MR in assessing preBT tumor maximum width in cervical cancer FIGO stage I/II. In more advanced stages TRUS seems to be slightly inferior to MR although maintaining a good agreement to gold standard imaging.

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