scholarly journals Comparing the Detection of Lymph Nodes Micrometastasis in Breast Cancer by the Hematoxylin and Eosin Staining Method (H&E) and the Immunohistochemical Method (IHC)

2017 ◽  
Vol 10 (11) ◽  
Author(s):  
Shokouh Taghipour Zahir ◽  
Shekofeh Shateri ◽  
Mohammad Baghi Yazdi ◽  
Pouria Barand ◽  
Elham Zare
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Staining Method ◽  
Immunohistochemical Method ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

scholarly journals INFILTRATING DUCTAL CARCINOMA OF MAMMARY GLAND IN A GERMAN SHEPHERD DOG

2012 ◽  
Vol 3 (2) ◽  
pp. 174-176
Author(s):  
R Sharmin ◽  
MM Rahman ◽  
M Masuduzzaman
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Staining Method ◽  
Ductal Carcinoma ◽  
Histopathological Study ◽  
German Shepherd ◽  
German Shepherd Dog ◽  
Infiltrating Ductal Carcinoma ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

A German Shepherd bitch of about 2.5 years old showed swollen ulcerated L-5 mammary gland. The affected L-5 gland and adjacent lymphnode were excised and processed for histopathological study with routine Hematoxylin and Eosin staining method. The ductular epithelial cells showed polymorphism and adenoid pattern growth of cells with hyperchromatric nuclei. Metastasis was not evident in the excised lymphnode. The neoplastic epithelial cells infiltrated the surround tissue. On the basis of histopathological finding this case was diagnosed as infiltrating ductal carcinoma.

scholarly journals Cytologic Features of Ventricular Tumors of the Central Nervous System: A Review with Emphasis on Diff-Quik Stained Smears

2021 ◽  
pp. 1-12
Author(s):  
Jose Antonio Jimenez-Heffernan ◽  
Federico Alvarez ◽  
Patricia Muñoz-Hernández ◽  
Carmen Bárcena ◽  
Daniel Azorin ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Staining Method ◽  
Ventricular System ◽  
Primary Tumors ◽  
Common Location ◽  
The Central Nervous System ◽  
Ventricular Tumors ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

<b><i>Background:</i></b> Neoplasms from the ventricular system share a common location but have highly variable histogenesis. Many are slowly growing tumors that behave in a benign fashion. They can be classified as primary and secondary tumors. The most common primary tumors are ependymomas, subependymomas, subependymal giant cell astrocytomas, central neurocytomas, choroid plexus tumors, meningiomas, germinomas, pineal parenchymal tumors, papillary tumors of the pineal region, chordoid gliomas, rosette-forming glioneuronal tumors of the fourth ventricle, and craniopharyngiomas. Pilocytic astrocytomas, medulloblastomas, and atypical teratoid/rhabdoid tumors often show secondary involvement of the ventricular system. <b><i>Summary:</i></b> Advances in neurosurgery have facilitated access to the ventricular system increasing the number of cases in which such tumors can be biopsied. In this context, cytology has been proven to be an extremely useful diagnostic tool during intraoperative pathologic consultations. Many ventricular tumors are infrequent, and the cytologic information available is limited. In this review, we describe the cytologic features of the uncommon ventricular tumors and report on unusual findings of the more common ones. For the cytologic evaluation of brain tumors, many neuropathologists prefer formalin fixation and hematoxylin and eosin staining. In this review, we highlight the cytologic findings as seen with Diff-Quik, a very popular staining method among cytopathologists. In fact, when pathologists are unfamiliar with cytology, it is common to request the assistance of cytopathologists during the evaluation of intraoperative procedures. <b><i>Key Message:</i></b> Ventricular tumors of the central nervous system comprise a group of heterogeneous tumors with very different cytologic features. The cytomorphology of these tumors, including rare entities, is often very characteristic, allowing a precise recognition during intraoperative pathologic consultations. Diff-Quik is a valuable staining method that can be used alone or as a complement to hematoxylin and eosin staining. Diff-Quik allows for clear visualization of the overall architecture, cytoplasmic details, and extracellular material.

scholarly journals A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and hematoxylin and eosin staining

2014 ◽  
Vol 6 (02) ◽  
pp. 084-090 ◽  
Author(s):  
Pinki Pandey ◽  
Alok Dixit ◽  
Aparna Tanwar ◽  
Anuradha Sharma ◽  
Sanjeev Mittal
Keyword(s):  
Staining Method ◽  
The Other ◽  
Staining Procedure ◽  
Significant Difference ◽  
Efficient Alternative ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
The Difference ◽  
Different Tissues

ABSTRACT Introduction: Our study presents a new deparaffinizing and hematoxylin and eosin (H and E) staining method that involves the use of easily available, nontoxic and eco-friendly liquid diluted dish washing soap (DWS) by completely eliminating expensive and hazardous xylene and alcohol from deparaffinizing and rehydration prior to staining, staining and from dehydration prior to mounting. The aim was to evaluate and compare the quality of liquid DWS treated xylene and alcohol free (XAF) sections with that of the conventional H and E sections. Materials and Methods: A total of 100 paraffin embedded tissue blocks from different tissues were included. From each tissue block, one section was stained with conventional H and E (normal sections) and the other with XAF H and E (soapy sections) staining method. Slides were scored using five parameters: Nuclear, cytoplasmic, clarity, uniformity, and crispness of staining. Z-test was used for statistical analysis. Results: Soapy sections scored better for cytoplasmic (90%) and crisp staining (95%) with a statistically significant difference. Whereas for uniformity of staining, normal sections (88%) scored over soapy sections (72%) (Z = 2.82, P < 0.05). For nuclear (90%) and clarity of staining (90%) total scored favored soapy sections, but the difference was not statistically significant. About 84% normal sections stained adequately for diagnosis when compared with 86% in soapy sections (Z = 0.396, P > 0.05). Conclusion: Liquid DWS is a safe and efficient alternative to xylene and alcohol in deparaffinization and routine H and E staining procedure. We are documenting this project that can be used as a model for other histology laboratories.

scholarly journals Adenomyoepithelioma of breast

2016 ◽  
Vol 6 (11) ◽  
pp. 962-964 ◽  
Author(s):  
N Sharma ◽  
U Manandhar ◽  
G Sayami
Keyword(s):  
Breast Cancer ◽  
Family History ◽  
Excisional Biopsy ◽  
Myoepithelial Cells ◽  
Left Breast ◽  
Pathological Diagnosis ◽  
Breast Lump ◽  
History Of ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

Adenomyoepithelioma of breast is a rare, benign proliferative tumor formed of proliferationof myoepithelial cells surrounding small epithelium lined spaces. We present two cases ofadenomyoepithelioma of the breast. The irst case was of a 23-years-old female with right breast lump and the second of a 41-year-old female with left breast lump. No patient had prior, coexistent carcinoma of either breast. None had any family history of breast cancer either. Excisional biopsy, Hematoxylin and Eosin staining and immunohistochemistry with S100 were performed. The pathological diagnosis was adenomyoepithelioma.

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