scholarly journals Detection of ISPa1328 and ISPpu21, Two Novel Insertion Sequences in the OprD Porin and blaIMP-1 Gene Among Metallo-Beta-Lactamase-Producing Pseudomonas aeruginosa Isolated From Burn Patients

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mehrzad Sadredinamin ◽  
Ali Hashemi ◽  
Hossein Goudarzi ◽  
Samira Tarashi ◽  
Neda Yousefi Nojookambari ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Insertion Sequences ◽  
Burn Patients ◽  
Beta Lactamase

Incidence and Susceptibility Pattern of Metallo-Beta-Lactamase Producers Among Pseudomonas aeruginosa Isolated From Burn Patients at Kurdistan Province

2012 ◽  
Vol 5 (3) ◽  
pp. 507-510 ◽  
Author(s):  
Enayatollah Kalantar ◽  
Vahideh Torabi ◽  
Himen Salimizand ◽  
Fariborz Soheili ◽  
Rashid Ramezanzadeh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Burn Patients ◽  
Beta Lactamase ◽  
Susceptibility Pattern

scholarly journals Burn Patients wounds Infected With Metallo-Beta-Lactamase-Producing Pseudomonas aeruginosa: Multidrug Resistant Strains

2014 ◽  
Vol 3 (2) ◽  
Author(s):  
Mojtaba Anvarinejad ◽  
Aziz Japoni ◽  
Noroddin Rafaatpour ◽  
Jalal Mardaneh ◽  
Pejman Abbasi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
Burn Patients ◽  
Beta Lactamase ◽  
Resistant Strains

Epidemiology and virulence of VIM-4 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from burn patients in eastern Algeria

Burns ◽  
2016 ◽  
Vol 42 (4) ◽  
pp. 906-918 ◽  
Author(s):  
Samah Meradji ◽  
Abouddihaj Barguigua ◽  
Mohamed cherif Bentakouk ◽  
Kaotar Nayme ◽  
Khalid Zerouali ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Burn Patients ◽  
Beta Lactamase ◽  
Eastern Algeria

Molecular Characterization of Methicillin Resistant and Extended Spectrum β-Lactamase Staphylococcus aureus Isolated from Burn Patients

2020 ◽  
pp. 145-151
Author(s):  
Shawnm Ahmed Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Vancomycin Resistance ◽  
World Health ◽  
Molecular Technique ◽  
Burn Patients ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

Antibiotic resistance has become a major world health challenge and has limited the ability of physician's treatment. Staphylococcus aureus the most notorious pathogens causes morbidity and mortality especially in burn patients. However, Staphylococcus aureus rapidly acquired resistance to multiple antibiotics. Vancomycin, a glycopeptide antibiotic remains a drug of choice for treatment of severe Methicillin Resistance S. aureus infections. This study aimed to detect the emergence of beta-lactam and glycopeptide resistance genes. 50 clinical specimens of S. aureus collected from burn patients in burn and plastic surgery units in Sulaimani-Iraq city. All specimens were confirmed to be positive for S. aureus. All the isolates were assessed for their susceptibility to different antibiotics depending on NCCL standards, followed by Extended Spectrum Beta Lactamase detection by double disk diffusion synergy test. The production of β- lactamases was evaluated in the isolated strains by several routine methods and polymerase chain reaction. Among the isolates 94% were Methicillin resistance and 34.28% were Extended Spectrum Beta Lactamase producer. PCR based molecular technique was done for the bla genes related to β- lactamase enzymes by the specific primers, as well as genes which related to reduced sensitivity to Vancomycin were detected. The results indicated that all isolated showed the PBP1, PBP2, PBP3, PBP4, trfA and trfB, graSR, vraS except the vraR gene and the prolonged therapy of Methicillin resistance infection with teicoplanin have been associated with progress of resistance and the rise of tecoplanin resistance may be a prologue to evolving Vancomycin resistance. In conclusion, beta-lactam over taking can rise Vancomycin- Intermediate S. aureus strains leading to appearance of Vancomycin resistance although the treatment of Vancomycin resistant infections is challenging.

scholarly journals Detection of extended spectrum beta-lactamase genes in Pseudomonas aeruginosa isolated from patients in rural Eastern Cape Province, South Africa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mojisola C. Hosu ◽  
Sandeep D. Vasaikar ◽  
Grace E. Okuthe ◽  
Teke Apalata
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
High Rate ◽  
Infection Prevention And Control ◽  
Beta Lactamase ◽  
Eastern Cape ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Resistance Patterns

AbstractThe proliferation of extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa represent a major public health threat. In this study, we evaluated the antimicrobial resistance patterns of P. aeruginosa strains and characterized the ESBLs and Metallo- β-lactamases (MBL) produced. Strains of P. aeruginosa cultured from patients who attended Nelson Mandela Academic Hospital and other clinics in the four district municipalities of the Eastern Cape between August 2017 and May 2019 were identified; antimicrobial susceptibility testing was carried out against thirteen clinically relevant antibiotics using the BioMérieux VITEK 2 and confirmed by Beckman autoSCAN-4 System. Real-time PCR was done using Roche Light Cycler 2.0 to detect the presence of ESBLs; blaSHV, blaTEM and blaCTX-M genes; and MBLs; blaIMP, blaVIM. Strains of P. aeruginosa demonstrated resistance to wide-ranging clinically relevant antibiotics including piperacillin (64.2%), followed by aztreonam (57.8%), cefepime (51.5%), ceftazidime (51.0%), piperacillin/tazobactam (50.5%), and imipenem (46.6%). A total of 75 (36.8%) multidrug-resistant (MDR) strains were observed of the total pool of isolates. The blaTEM, blaSHV and blaCTX-M was detected in 79.3%, 69.5% and 31.7% isolates (n = 82), respectively. The blaIMP was detected in 1.25% while no blaVIM was detected in any of the strains tested. The study showed a high rate of MDR P. aeruginosa in our setting. The vast majority of these resistant strains carried blaTEM and blaSHV genes. Continuous monitoring of antimicrobial resistance and strict compliance towards infection prevention and control practices are the best defence against spread of MDR P. aeruginosa.

The frequency of genes encoding exotoxin A and exoenzyme S in Pseudomonas aeruginosa strains isolated from burn patients

Burns ◽  
2016 ◽  
Vol 42 (5) ◽  
pp. 1116-1120 ◽  
Author(s):  
Azar Dokht Khosravi ◽  
Fatemeh Shafie ◽  
Effat Abbasi Montazeri ◽  
Soodabeh Rostami
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Burn Patients ◽  
Exotoxin A ◽  
Exoenzyme S ◽  
Genes Encoding
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