scholarly journals Selective Enrichment Broth and Chromogenic Agar for Detection of Enterohemorrhagic Escherichia coli O26, O103, O111, O121, O145 and O157

2015 ◽  
Vol 32 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Yukiko HARA-KUDO ◽  
Junko ISOBE ◽  
Ichiro FURUKAWA ◽  
Fumio GONDAIRA ◽  
Jun TERAJIMA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Chromogenic Agar ◽  
Selective Enrichment Broth

scholarly journals SEL, a Selective Enrichment Broth for Simultaneous Growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes

2008 ◽  
Vol 74 (15) ◽  
pp. 4853-4866 ◽  
Author(s):  
Hyochin Kim ◽  
Arun K. Bhunia
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Enterica ◽  
Single Experiment ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
E Coli ◽  
Multiplex Pcr Assay ◽  
Selective Enrichment Broth ◽  
Simultaneous Growth

ABSTRACT Multipathogen detection on a single-assay platform not only reduces the cost for testing but also provides data on the presence of pathogens in a single experiment. To achieve this detection, a multipathogen selective enrichment medium is essential to allow the concurrent growth of pathogens. SEL broth was formulated to allow the simultaneous growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes. The results were compared to those obtained with the respective individual selective enrichment broths, Rappaport-Vassiliadis (RV) for S. enterica, modified E. coli broth with 20 mg of novobiocin/liter for E. coli O157:H7, and Fraser broth for L. monocytogenes, and a currently used universal preenrichment broth (UPB). The growth of each pathogen in SEL inoculated at 101 or 103 CFU/ml was superior to that in the respective individual enrichment broth, except in the case of RV, in which Salmonella cells inoculated at both concentrations grew equally well. In mixed-culture experiments with cells of the three species present in equal concentrations or at a 1:10:1,000 ratio, the overall growth was proportional to the initial inoculation levels; however, the growth of L. monocytogenes was markedly suppressed when cells of this species were present at lower concentrations than those of the other two species. Further, SEL was able to resuscitate acid- and cold-stressed cells, and recovery was comparable to that in nonselective tryptic soy broth containing 6% yeast extract but superior to that in the respective individual selective broths. SEL promoted the growth of all three pathogens in a mixture in ready-to-eat salami and in turkey meat samples. Moreover, each pathogen was readily detected by a pathogen-specific immunochromatographic lateral-flow or multiplex PCR assay. Even though the growth of each pathogen in SEL was comparable to that in UPB, SEL inhibited greater numbers of nontarget organisms than did UPB. In summary, SEL was demonstrated to be a promising new multiplex selective enrichment broth for the detection of the three most prominent food-borne pathogens by antibody- or nucleic acid-based methods.

scholarly journals Detection of Burkholderia pseudomallei in Sputum using Selective Enrichment Broth and Ashdown’s Medium at Kampong Cham Provincial Hospital, Cambodia

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 302 ◽  
Author(s):  
Somary Nhem ◽  
Joanne Letchford ◽  
Chea Meas ◽  
Sovanndeth Thann ◽  
James C. McLaughlin ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Low Frequency ◽  
Culture Methods ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Provincial Hospital ◽  
Testing Protocol ◽  
Standard Culture ◽  
Diagnostic Microbiology ◽  
Selective Enrichment Broth

Melioidosis infection, caused by Burkholderia pseudomallei, is increasingly reported in Cambodia. We hypothesized that implementation of an enhanced sputum testing protocol in a provincial hospital diagnostic microbiology laboratory would increase detection of B. pseudomallei. We tested 241 sputum specimens that were deemed acceptable for culture, comparing culture in selective enrichment broth followed by sub-culture on Ashdown’s medium to standard culture methods. Two specimens (0.8%) were positive for B. pseudomallei using the enhanced protocol whereas one specimen (0.4%) was positive using standard methods. These findings demonstrate that B. pseudomallei is rarely detected in sputum at this hospital. The low frequency of B. pseudomallei in sputum specimens precludes drawing any conclusions about the relative benefits of an enhanced sputum testing protocol at this site. Promoting clinician awareness of the infection and encouraging utilization of diagnostic microbiology services are likely to be important factors in facilitating identification of melioidosis.

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