scholarly journals Survival of Campylobacter jejuni in Frozen Chicken Meat and Genetic Analysis of Isolates by Pulsed-Field Gel Electrophoresis

2005 ◽  
Vol 22 (2) ◽  
pp. 59-65 ◽  
Author(s):  
Kazuaki ONO ◽  
Yoko ANDO ◽  
Fumihiko KAWAMORI ◽  
Yukie OZEKI ◽  
Keiko YANAGAWA
Keyword(s):  
Genetic Analysis ◽  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Chicken Meat ◽  
Pulsed Field

scholarly journals Clonal Population Structure and Antimicrobial Resistance of Campylobacter jejuni in Chicken Meat from Belgium

2009 ◽  
Vol 75 (13) ◽  
pp. 4264-4272 ◽  
Author(s):  
Ihab Habib ◽  
William G. Miller ◽  
Mieke Uyttendaele ◽  
Kurt Houf ◽  
Lieven De Zutter
Keyword(s):  
Campylobacter Jejuni ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Clonal Complex ◽  
Genotypic Diversity ◽  
Pulsed Field Gel Electrophoresis ◽  
Chicken Meat ◽  
Clonal Population ◽  
Clonal Population Structure ◽  
Sequence Types

ABSTRACT Campylobacter jejuni is one of the most important causes of human diarrhea worldwide. In the present work, multilocus sequence typing was used to study the genotypic diversity of 145 C. jejuni isolates from 135 chicken meat preparations sampled across Belgium. Isolates were further typed by pulsed-field gel electrophoresis, and their susceptibilities to six antimicrobials were determined. Fifty-seven sequence types (STs) were identified; 26.8% of the total typed isolates were ST-50, ST-45, or ST-257, belonging to clonal complex CC-21, CC-45, or CC-257, respectively. One clonal group comprised 22% (32/145) of all isolates, originating from five different companies and isolated over seven sampling months. Additionally, 53.1% of C. jejuni isolates were resistant to ciprofloxacin, and 48.2% were resistant to tetracycline; 28.9% (42/145) of all isolates were resistant to both ciprofloxacin and tetracycline. The correlation between certain C. jejuni clonal groups and resistance to ciprofloxacin and tetracycline was notable. C. jejuni isolates assigned to CC-21 (n = 35) were frequently resistant to ciprofloxacin (65.7%) and tetracycline (40%); however, 90% (18/20) of the isolates assigned to CC-45 were pansusceptible. The present study demonstrates that certain C. jejuni genotypes recur frequently in the chicken meat supply. The results of molecular typing, combined with data on sample sources, indicate a possible dissemination of C. jejuni clones with high resistance to ciprofloxacin and/or tetracycline. Whether certain clonal groups are common in the environment and repeatedly infect Belgian broiler flocks or whether they have the potential to persist on farms or in slaughterhouses needs further investigation.

scholarly journals A Three-Year Study of Campylobacter jejuni Genotypes in Humans with Domestically Acquired Infections and in Chicken Samples from the Helsinki Area

2000 ◽  
Vol 38 (5) ◽  
pp. 1998-2000 ◽  
Author(s):  
Marja-Liisa Hänninen ◽  
Päivikki Perko-Mäkelä ◽  
Anna Pitkälä ◽  
Hilpi Rautelin
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Peak Level ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Stool Samples

Campylobacter jejuni isolates from stool samples of patients with domestically acquired sporadic infections and from chicken from retail shops were studied during seasonal peaks from June to September over a 3-year period from 1996 to 1998. A large number of pulsed-field gel electrophoresis (PFGE) genotypes (a combinedSmaI-SacII pattern) were identified each year. Certain genotypes persisted for the whole study period, and predominant genotypes represented 28 to 52% of the strains during a restricted period of time. The peak level of positive chicken samples was between July and August of each study year, when 10 to 33% of the samples were positive for campylobacter. The same PFGE genotypes found in humans were also detected in the chicken samples. This suggests that common genotypes were circulating in the area.

scholarly journals Genetic Analysis of Multiple Vancomycin-ResistantEnterococcus Isolates Obtained Serially from Two Long-Term-Care Patients

1998 ◽  
Vol 36 (7) ◽  
pp. 2105-2108 ◽  
Author(s):  
Dianna J. Schoonmaker ◽  
Lawrence H. Bopp ◽  
Aldona L. Baltch ◽  
Raymond P. Smith ◽  
Mary Ellen Rafferty ◽  
...  
Keyword(s):  
High Risk ◽  
Genetic Analysis ◽  
Gel Electrophoresis ◽  
Long Term Care ◽  
Pulsed Field Gel Electrophoresis ◽  
Risk Of Infection ◽  
Term Care ◽  
Pulsed Field ◽  
Vancomycin Resistant

Fifty-eight vancomycin-resistant enterococcal isolates were obtained from two patients over 9 weeks. Numerous pulsed-field gel electrophoresis fingerprinting types were isolated from each patient. By PCR, all isolates were vanA +. However, many isolates from patient B were found to lack vanA by hybridization. Our results demonstrate the importance of examining multiple isolates, especially from patients who are at high risk of infection.

scholarly journals Validity of SmaI-defined genotypes of Campylobacter jejuni examined by SalI, KpnI, and BamHI polymorphisms: evidence of identical clones infecting humans, poultry, and cattle

1998 ◽  
Vol 120 (3) ◽  
pp. 231-237 ◽  
Author(s):  
S. L. W. ON ◽  
E. M. NIELSEN ◽  
J. ENGBERG ◽  
M. MADSEN
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Restriction Site ◽  
Epidemiological Studies ◽  
Pulsed Field Gel Electrophoresis ◽  
Genetic Differences ◽  
Genetic Fingerprinting ◽  
Molecular Subtyping ◽  
Pulsed Field ◽  
Heat Stable

We describe here an examination of the validity of molecular types of Campylobacter jejuni as defined by separation of SmaI-digested DNA using pulsed-field gel electrophoresis (PFGE), recently suggested as part of a molecular subtyping scheme. Thirty-four Danish strains from humans, water, poultry and cattle were assigned to one of six SmaI ‘profile groups’ (PGs), with two additional strains included as genotypically distinct controls. The interstrain relationships were reexamined by PFGE of SalI, KpnI and BamHI-digested DNA, and also by serotyping with heat-stable antigens. All outbreak-related strains were indistinguishable by all criteria, as were two sets of two randomly-isolated human strains. Two groups of indistinguishable isolates contained randomly isolated strains from more than one source (poultry, humans and/or cattle), a finding with significant epidemiological connotations. All ‘genetically identical’ strains belonged to the same serotype, whereas genetic differences were detected between strains assigned to the same SmaI PG but differing in serotype. We conclude that PFGE-based genetic fingerprinting can yield invaluable data for epidemiological studies of sporadic C. jejuni infection, but that results based on one restriction site polymorphism must be checked with another enzyme.

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