scholarly journals Preparation of l-lactate Biosensor that uses Polyion Complex Membrane Containing Peroxidase and Ferrocene

2008 ◽  
Vol 76 (8) ◽  
pp. 552-554
Author(s):  
Kenta IWASAWA ◽  
Mika EGUCHI ◽  
Katsuhiro UNO ◽  
Soichi YABUKI
Keyword(s):  
Polyion Complex ◽  
Complex Membrane

Peroxidase and Toluidine Blue-Incorporated DNA Bio-Polyion Complex Film as Biosensing Layer for Hydrogen Peroxide Determination

2013 ◽  
Vol 750 ◽  
pp. 84-87 ◽  
Author(s):  
Ting Ting Gu ◽  
Hong Qi Xia ◽  
Jian Li Wang ◽  
Da Wei Yang ◽  
Yang Zhao ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Cathodic Current ◽  
Catalytic Center ◽  
Polyion Complex ◽  
Double Stranded Dna ◽  
Electron Transfer Rate Constant ◽  
Complex Membrane ◽  
Highly Sensitive ◽  
Electrochemical Redox ◽  
Complex Film

Horseradish peroxidase (HRP) and toluidine blue (TB) were incorporated in polyion complex membrane composed of double stranded DNA(dsDNA) and chitosan prepared on the surface of an glassy carbon (GC) disk electrode to fabricate highly sensitive and selective reagentless H2O2 biosensor. The embedded-TB in the DNA/chitosan membrane exhibited excellent electrochemical redox property with an electron transfer rate constant of 3.12 ± 0.5 sec−1, and shuttled electron effectively from the base GC electrode to catalytic center of the HRP. Under the applied potential of -0.22V (versus Ag/AgCl) and pH 7.0, the resulting electrode (HRP/DNA–TB/chitosan/GCE) exhibited rapid (<10 s) and sensitive response to H2O2. The calibration curve of H2O2, plotting steady-state cathodic current versus H2O2 concentration, was linear up to 0.1mM with a detection limit of 1 μM H2O2 (S/N = 3). The H2O2 response was scarcely interfered by ascorbic acid and uric acid, which potentially reduce oxidized intermediate of the HRP and interfere with the response of peroxidase-based electrodes.

scholarly journals Amperometric Alcohol-Sensing Electrode Based on a Polyion Complex Membrane Containing Alcohol Oxidase.

1997 ◽  
Vol 13 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Fumio MIZUTANI ◽  
Soichi YABUKI ◽  
Seiichiro IIJIMA
Keyword(s):  
Alcohol Oxidase ◽  
Polyion Complex ◽  
Complex Membrane
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