Cytological investigation of deep-layer squamous cells in oral cytology

2021 ◽  
Vol 60 (1) ◽  
pp. 8-14
Author(s):  
Masaaki SUEMITSU ◽  
Takashi MATSUMOTO ◽  
Hiroyuki SETO ◽  
Mitsuko NAKAYAMA ◽  
Miyuki MORIKAWA ◽  
...  
Keyword(s):  
Deep Layer ◽  
Cytological Investigation ◽  
Squamous Cells ◽  
Oral Cytology

Galectin-1 is a useful marker for detecting neoplastic squamous cells in oral cytology smears

2016 ◽  
Vol 52 ◽  
pp. 101-109 ◽  
Author(s):  
Yuri Noda ◽  
Yuko Kondo ◽  
Manabu Sakai ◽  
Sunao Sato ◽  
Mitsunobu Kishino
Keyword(s):  
Squamous Cells ◽  
Oral Cytology

Incidence of Cytological Abnormalities in Pap's Smear in HIV Positive Women

2018 ◽  
Vol 2 (S1) ◽  
pp. e000136
Author(s):  
Suhana Jotva ◽  
Hemani Desai ◽  
Hansa Goswami
Keyword(s):  
Trichomonas Vaginalis ◽  
Clinical History ◽  
Hiv Positive ◽  
Squamous Intraepithelial Lesions ◽  
Squamous Cells ◽  
Medical College ◽  
Hiv Positive Women ◽  
Cytological Abnormalities ◽  
Intraepithelial Lesions ◽  
Hiv Negative

Aims and Objectives: The aim of present study is to estimate the frequency of abnormal PAP’s smears and mainly to detect precancerous and cancerous lesions as well as inflammatory lesions in HIV infected women. Methodology: Our study was a retrospective study of total 130 cases and PAP’s smears were examined in cytology section in Department of Pathology, BJ Medical College, Ahmedabad from 1st March 2017 to 31st August 2017. Both HIV positive and HIV negative patients were included in the study. 80 patients were HIV negative and 50 patients were HIV positive. The clinical history and relevant parameters were noted. All the smears were processed by a conventional method using Papanicolaou stain.  Results: Out of 130 cases, 118 cases were reported negative for intraepithelial lesions or malignancy (NILM). Five cases were positive for squamous intraepithelial lesions (SIL) out of which four were HIV positive. Seven cases were of atypical squamous cells of undetermined significance (ASCUS) out of which 5 were HIV positive. Three cases were showing bacterial vaginosis. 1 case was showing Trichomonas Vaginalis. 19 cases were showing changes of non-specific inflammation. 6 cases were showing atrophic changes.  Conclusion: HIV infected women are at more risk to develop cervical cytological abnormalities. The study revealed a maximum number of non-specific inflammatory smears followed by smears showing atrophic changes. Smears suspicious for malignancy (ASCUS) and smears showing squamous intraepithelial lesions (SIL) were more common in HIV positive patients. Thus regular PAP’s smears screening as recommended by National AIDS Control Organization (NACO) will help in early detection of cervical abnormalities in HIV positive women. 

Atypical squamous cells of indeterminate significance (ASC-US): follow-up of assay in Instituto Adolfo Lutz

2018 ◽  
Vol 50 (3) ◽  
Author(s):  
Denise Andrade Rosendo ◽  
Sandra Lorente ◽  
Clebia Maria dos Santos ◽  
Gustavo Martins Ferreira ◽  
Lethicia Moura Canello ◽  
...  
Keyword(s):  
Squamous Cells

scholarly journals Morphology of the Undersurface of the Anterolateral Acromion and Its Relationship to Surrounding Structures

2021 ◽  
Vol 9 (1) ◽  
pp. 232596712097748
Author(s):  
Yusuke Ueda ◽  
Akimoto Nimura ◽  
Keisuke Matsuki ◽  
Kumiko Yamaguchi ◽  
Hiroyuki Sugaya ◽  
...  
Keyword(s):  
Deep Layer ◽  
Micro Computed Tomography ◽  
Middle Aged ◽  
Aged Patients ◽  
Coracoacromial Ligament ◽  
3 Dimensional ◽  
Bony Surface ◽  
Inferior Aspect ◽  
Histologic Evaluation ◽  
Arthroscopic Subacromial Decompression

Background: A better understanding of the morphology underneath the acromion is needed to prevent complications after arthroscopic subacromial decompression. The precise correlations between the morphologic features underneath the acromion and the surrounding structures including the attachment of the coracoacromial ligament (CAL) and the origin of the deltoid middle head have not yet been determined in the absence of artifacts on the bony surface caused by dissection techniques. Moreover, anatomic findings in previous studies using only older-aged cadavers or dried bones may not reflect the morphologic features of younger and healthy specimens. Purpose: To characterize the anterolateral structures morphologically in the inferior aspect of the acromion, assess the relationships of these structures with surrounding structures without dissection artifacts on the bony surface, and verify the cadaveric data in the asymptomatic shoulders of living middle-aged patients. Study Design: Descriptive laboratory study. Methods: We initially analyzed the relationship between the morphology of the anterolateral structures and surrounding structures in 18 cadaveric shoulders (mean age, 81.8 years), 15 of which were subjected to macroscopic investigation of the CAL attachment and 3-dimensional micro—computed tomography investigation with radiopaque markers and 3 of which were subjected to histologic examination. We also analyzed the morphology underneath the anterolateral acromion in 24 asymptomatic shoulders of middle-aged patients (mean age, 54.8 years) to verify the cadaveric data. In both the cadaveric shoulders and the asymptomatic shoulders of live patients, the long axis, width, and height of the anterolateral prominence were measured by use of 3-dimensional CT imaging. Results: In cadavers, the anterolateral prominence underneath the acromion corresponded to the attachment of the CAL. Histologic evaluation revealed that the CAL was continuous to the deep layer of the deltoid middle head in the lateral acromion. The study in asymptomatic shoulders of middle-aged patients revealed bony prominences similar to those observed in cadavers. Conclusion: The anterolateral prominence, which corresponds to the attachment of the CAL below the acromion, may be a native structure below the acromion. Moreover, the CAL is continuous to the deep layer of the deltoid middle head in the lateral acromion.

scholarly journals A non-toxic, reversibly released imaging probe for oral cancer that is derived from natural compounds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Magda Ghanim ◽  
Nicola Relitti ◽  
Gavin McManus ◽  
Stefania Butini ◽  
Andrea Cappelli ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Progression ◽  
Intraoperative Imaging ◽  
Critical Role ◽  
Water Soluble ◽  
Stem Cell Marker ◽  
Squamous Cells ◽  
Tissue Identification ◽  
Tumorigenic Potential ◽  
The Impact

AbstractCD44 is emerging as an important receptor biomarker for various cancers. Amongst these is oral cancer, where surgical resection remains an essential mode of treatment. Unfortunately, surgery is frequently associated with permanent disfigurement, malnutrition, and functional comorbidities due to the difficultly of tumour removal. Optical imaging agents that can guide tumour tissue identification represent an attractive approach to minimising the impact of surgery. Here, we report the synthesis of a water-soluble fluorescent probe, namely HA-FA-HEG-OE (compound 1), that comprises components originating from natural sources: oleic acid, ferulic acid and hyaluronic acid. Compound 1 was found to be non-toxic, displayed aggregation induced emission and accumulated intracellularly in vesicles in SCC-9 oral squamous cells. The uptake of 1 was fully reversible over time. Internalization of compound 1 occurs through receptor mediated endocytosis; uniquely mediated through the CD44 receptor. Uptake is related to tumorigenic potential, with non-tumorigenic, dysplastic DOK cells and poorly tumorigenic MCF-7 cells showing only low intracellular levels and highlighting the critical role of endocytosis in cancer progression and metastasis. Together, the recognised importance of CD44 as a cancer stem cell marker in oral cancer, and the reversible, non-toxic nature of 1, makes it a promising agent for real time intraoperative imaging.

scholarly journals A Hypothesis for the Redevelopment of Warm-Core Cyclones over Northern Australia

2008 ◽  
Vol 136 (10) ◽  
pp. 3863-3872 ◽  
Author(s):  
Kerry Emanuel ◽  
Jeff Callaghan ◽  
Peter Otto
Keyword(s):  
Heat Transfer ◽  
Thermal Diffusivity ◽  
Tropical Cyclones ◽  
Deep Layer ◽  
Heat Fluxes ◽  
Northern Australia ◽  
One Dimensional ◽  
Warm Core ◽  
Vertical Heat ◽  
Underlying Soil

Tropical cyclones moving inland over northern Australia are occasionally observed to reintensify, even in the absence of well-defined extratropical systems. Unlike cases of classical extratropical rejuvenation, such reintensifying storms retain their warm-core structure, often redeveloping such features as eyes. It is here hypothesized that the intensification or reintensification of these systems, christened agukabams, is made possible by large vertical heat fluxes from a deep layer of very hot, sandy soil that has been wetted by the first rains of the approaching systems, significantly increasing its thermal diffusivity. To test this hypothesis, simulations are performed with a simple tropical cyclone model coupled to a one-dimensional soil model. These simulations suggest that warm-core cyclones can indeed intensify when the underlying soil is sufficiently warm and wet and are maintained by heat transfer from the soil. The simulations also suggest that when the storms are sufficiently isolated from their oceanic source of moisture, the rainfall they produce is insufficient to keep the soil wet enough to transfer significant quantities of heat, and the storms then decay rapidly.

scholarly journals The Role of CD68 (+) Histiocytic Macrophages in Nasal Polyp Development

2020 ◽  
Author(s):  
Nuray Bayar Muluk ◽  
Osman Kürşat Arikan ◽  
Pınar Atasoy ◽  
Rahmi Kiliç ◽  
Eda Tuna Yalçinozan
Keyword(s):  
Nasal Cavity ◽  
Nasal Polyp ◽  
Nasal Polyps ◽  
Deep Layer ◽  
Ethmoid Sinus ◽  
Adult Patients ◽  
Control Group ◽  
Study Group ◽  
Deep Layers

Abstract Objectives The aim of this study was to investigate the role of CD68 (+) histiocytic macrophages (H-M) in the nasal polyp pathogenesis. Materials and Methods The study group consisted of 24 adult patients with nasal polyposis. The control group consisted of 11 adult patients without nasal polyps. A total of 36 nasal polyp samples (10-nasal cavity, 10-maxillary sinus, and 16-ethmoid sinus) from the study group and 11 inferior turbinate samples from the control group were analyzed by immunohistochemical staining, with monoclonal antibodies against CD68 (+) H-M. Results CD68 positivity was significantly higher than the control group in the subepithelial (SE) layer of the ethmoid sinus, and deep layers of nasal cavity, maxillary, and ethmoid sinuses. In SE and deep layers of ethmoid and maxillary sinuses, CD68 positivity was significantly higher than that of the epithelial layer. In the deep layer, histiocytic macrophages tended to gather around eosinophils. Conclusion The high numbers of CD68 (+) histiocytic macrophages mainly located in deep layer of lamina propria may be responsible for the phagocytosis of eosinophils within the polyp tissue. Therefore, it may be concluded that increased macrophages in nasal polyps do not trigger the growth of nasal polyps. Instead, they may serve to reduce the number of eosinophils in already-developed nasal polyps.

scholarly journals Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology

2016 ◽  
Vol 50 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Alves Mônica Ghislaine Oliveira ◽  
Mario Pérez-Sayáns ◽  
Maria-Elena Padín-Iruegas ◽  
Maria Dolores Reboiras-López ◽  
José Manuel Suarez-Peñaranda ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Rna Extraction ◽  
Extraction Methods ◽  
Rna Extraction Methods ◽  
Oral Cytology
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