Improvement in situ PCR-Detection of single-copy HPV-DNA-

2010 ◽  
Vol 49 (6) ◽  
pp. 393-399
Author(s):  
Kaori OKAYAMA ◽  
Mitsuaki OHKODO ◽  
Tomoko KUMAGAI ◽  
Hiromi YABUSAKI ◽  
Haruki YOSHINAGA ◽  
...  
Keyword(s):  
Single Copy ◽  
Pcr Detection ◽  
In Situ Pcr ◽  
Hpv Dna

Localization of HPV and SC35 Domains in SiHa and CaSki Cells by TSA Using Fluorescent Tyramides

1997 ◽  
Vol 3 (S2) ◽  
pp. 141-142
Author(s):  
K.E. Adler ◽  
P.T. Moen ◽  
T.J. Erickson ◽  
M.N. Bobrow
Keyword(s):  
Spatial Relationship ◽  
High Sensitivity ◽  
Single Copy ◽  
Hpv Dna ◽  
Siha Cells ◽  
Specialized Equipment ◽  
Related Proteins ◽  
Relationship Of ◽  
Antigen 5

Detection of integrated HPV DNA in SiHa and CaSki cells has been demonstrated by in situ PCR (1,2,3) and more recently TSA (4). These reports focused on high sensitivity single copy HPV detection using chromogenic techniques. Here we utilize high resolution and high sensitivity fluorescent methods of detection to analyze the spatial relationship of HPV DNA with respect to subnuclear domains enriched in splicing-related proteins, shown here by immunofluorescent detection of the SC-35 antigen (5). In situhybridization was performed using conventional reagents and the resulting probe signal amplified using fluorescent labeled tyramides (6). In contrast to techniques such as in situ PCR which require specialized procedures and equipment, detection of HPV DNA in SiHa cells was rapidly and routinely achieved using no specialized equipment. In addition, high target resolution was maintained, in contrast to in situ PCR, which is plagued by signal dispersion and difficult localization (7).

In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease

2003 ◽  
Vol 4 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Ivan Cordova ◽  
Phil Jones ◽  
Nigel A. Harrison ◽  
Carlos Oropeza
Keyword(s):  
Pcr Detection ◽  
In Situ Pcr ◽  
Lethal Yellowing Disease ◽  
Lethal Yellowing ◽  
Yellowing Disease

In Situ PCR Detection of HIV Expression in the Human Placenta

2003 ◽  
pp. 75-86
Author(s):  
Asad U. Sheikh ◽  
Bruno M. Polliotti ◽  
Richard K. Miller
Keyword(s):  
Human Placenta ◽  
Pcr Detection ◽  
In Situ Pcr

scholarly journals RT in situ PCR detection of MART-1 and TRP-2 mRNA in formalin-fixed, paraffin-embedded tissues of melanoma and nevi

2008 ◽  
Vol 21 (3) ◽  
pp. 326-333 ◽  
Author(s):  
Eijun Itakura ◽  
Rong-Rong Huang ◽  
Duan-Ren Wen ◽  
Eberhard Paul ◽  
Peter H Wünsch ◽  
...  
Keyword(s):  
Pcr Detection ◽  
In Situ Pcr ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Fluorescent RT in situ PCR detection of MRP1 mRNA in human HCV infected liver

10.4081/1620 ◽  
2009 ◽  
Vol 45 (1) ◽  
pp. 105 ◽  
Author(s):  
L Pascolo ◽  
S Bogoni ◽  
A Spanò ◽  
E Demori ◽  
A Amoroso ◽  
...  
Keyword(s):  
Pcr Detection ◽  
In Situ Pcr ◽  
Infected Liver

scholarly journals Visualization and Direct Counting of Individual Denitrifying Bacterial Cells in Soil by nirK-Targeted Direct in situ PCR

2011 ◽  
Vol 26 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Noriko Ryuda ◽  
Tomoyoshi Hashimoto ◽  
Daisuke Ueno ◽  
Koichi Inoue ◽  
Takashi Someya
Keyword(s):  
Bacterial Cells ◽  
In Situ Pcr ◽  
Direct Counting
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