scholarly journals Immunocytochemical study of smooth muscle actin for the detection of myoepithelial cells in aspiration biopsy cytology of the breast.

1995 ◽  
Vol 34 (3) ◽  
pp. 413-419
Author(s):  
Hitoshi ITO ◽  
Yohko MIYAJIMA ◽  
Reiko SHINODA ◽  
Shinobu UMEMURA ◽  
Yutaka TSUTSUMI ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Myoepithelial Cells ◽  
Aspiration Biopsy ◽  
Muscle Actin ◽  
Immunocytochemical Study ◽  
Aspiration Biopsy Cytology

scholarly journals p21/waf1 and Smooth-Muscle Actin α Expression in Stromal Fibroblasts of Oral Cancers

2010 ◽  
Vol 33 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Ioulia Chatzistamou ◽  
Nikolina Dioufa ◽  
George Trimis ◽  
Alexandra Sklavounou ◽  
Christos Kittas ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Actin ◽  
Myoepithelial Cells ◽  
Mrna Levels ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Stromal Fibroblasts ◽  
Oral Cancers ◽  
Malignant Lesions

Background: Concerted alterations between stromal fibroblasts and neoplastic cells underline the carcinogenic process. Activation of alpha-smooth muscle actin (SMA) expression, a cytoskeleton protein normally expressed only in myoepithelial cells, is considered a landmark for the activation of stromal fibroblasts with little however being known regarding the mechanism governing the expression of SMA in the stroma.Methods: We have evaluated by immunohistochemistry the expression of SMA in the stroma of oral malignant and pre-malignant lesions, in association with the expression of p53 and p21 tumor suppressors that were shown previously to be deregulated and/or mutated in stromal fibroblasts of various cancers. The effects of p21 knockdown in SMA expression and cell migration and the mRNA levels of endogenous p21 in fibroblasts co-cultured with cancer cells were also assessed.Results: We found that both p21 and SMA expression was elevated in the stroma, but not the epithelium, of malignant as compared to pre-malignant lesions. We also noted that the expression of both was positively correlated, implying that SMA expression may be regulated by p21. Consistently with this notion we found that siRNA-mediated p21 suppression resulted in the reduction of SMA levels and also inhibited cell migration.Conclusion: Our results show that p21 deregulation is associated with the activation of stromal fibroblasts of oral cancers by a mechanism that involves the stimulation of SMA expression.

Immunohistochemical investigation into the distribution pattern of myoepithelial cells in the bovine mammary gland

1997 ◽  
Vol 64 (2) ◽  
pp. 197-205 ◽  
Author(s):  
EVA HELLMÉN ◽  
AXEL ISAKSSON
Keyword(s):  
Smooth Muscle ◽  
Mammary Gland ◽  
Distribution Pattern ◽  
Basal Cell ◽  
Cell Layer ◽  
Smooth Muscle Actin ◽  
Myoepithelial Cells ◽  
Muscle Actin ◽  
Bovine Mammary Gland ◽  
Basal Cell Layer

The distribution pattern of myoepithelial cells in the bovine mammary gland was investigated by an immunohistochemical technique, using monoclonal antibodies against cytokeratins 5, 6 and 18 and cytokeratins 8 and 14 and against α-smooth-muscle actin filaments. Myoepithelial cells were shown to be present as a continuous basal cell layer in the intralobular ducts, as discontinuous cell rows in the basal cell layer of the interlobular ducts, and as single cells dispersed in the basal cell layer of the quarter cisterns, while they were apparently absent in the teat cisterns. Unlike the case with myoepithelial cells of the human breast, anti-cytokeratin 14 was less specific as a marker of bovine myoepithelial cells than was anti-α-smooth-muscle actin.

scholarly journals Specific demonstration of myoepithelial cells by anti-alpha smooth muscle actin antibody.

1988 ◽  
Vol 36 (6) ◽  
pp. 659-663 ◽  
Author(s):  
P Gugliotta ◽  
A Sapino ◽  
L Macrí ◽  
O Skalli ◽  
G Gabbiani ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cells ◽  
Salivary Glands ◽  
Smooth Muscle Actin ◽  
Practical Interest ◽  
Myoepithelial Cells ◽  
Basal Cells ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Microfilament Bundles

The myoepithelial cells of the sweat, mammary, tracheobronchial, and salivary glands are specifically identified by monoclonal antibody alpha-SM-1, which recognizes alpha smooth muscle actin and not the other actin isoforms. Basal or "reserve" cells in the stratified epithelia and excretory ducts of the salivary glands are negative, as well as all other epithelial cells in various organs. The reaction can be performed in routinely fixed and embedded tissues and is of practical interest in diagnostic histopathology. In immunoelectron cytochemistry, alpha-SM-1 antibody binds to the microfilament bundles in myoepithelial cells of the breast, but does not stain luminal cells and occasional basally located epithelial cells. These basal cells are morphologically and immunocytochemically distinct from the myoepithelial cells, and their nature and significance remain to be clarified.

scholarly journals Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

2021 ◽  
Vol 11 (8) ◽  
pp. 3524
Author(s):  
Azeem Ul Yaqin Syed ◽  
Muhammad A. Ahmed ◽  
Eman I. AlSagob ◽  
Mansour Al-Askar ◽  
Abdulrahman M. AlMubarak ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Control Cell ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Muscle Actin ◽  
Catha Edulis ◽  
Squamous Carcinoma Cells ◽  
Dose Dependent

The aim was to determine the cytotoxicity of Khat (Catha edulis (Vahl) Forssk. ex Endl) on normal oral fibroblasts (NOFs) and SCC4 (squamous carcinoma cells) along with expression of α-smooth muscle actin (α-SMA) in fibroblasts. Khat filtrate was prepared to obtain a concentrated viscous solution. NOFs and SCC4 cells were cultured in biological cabinets and were grown in Dulbeccos’ modified Eagles medium. Frozen cells were thawed at 37 °C and cell seeding was performed. NOFs and SCC4 cells were seeded on 96 well plates and allowed to attach. The medium was removed and a fresh medium containing different concentrations of Khat was added. The group without Khat served as a negative control and 4% paraformaldehyde as the positive control. Cell viability was assessed using the MTT assay and effect of Khat on fibroblast and SCC4 phenotypes was evaluated by immunostaining. Analysis of variance was used to assess data (p < 0.05). NOF 316 showed cell death in response to 4% paraformaldehyde, 12.5, 6.25, and 3.12 mg/mL of Khat. The highest concentration of Khat (25 mg/mL) failed to cause cytotoxicity of NOF 316. NOF 319 and NOF 26 displayed cell death at all concentrations of Khat, however, cytotoxicity was not dose dependent. NOF 18 and SCC4 cells showed dose-dependent cell death. NOF 316 showed α-SMA expression after 1 mg/mL of Khat exposure. Not all fibroblasts were α-SMA-positive, suggesting specific activation of a subset of fibroblasts. Khat is cytotoxic to NOF and SCC4 cells. Furthermore, it can also cause activation and phenotypic changes in oral fibroblasts, indicating a potential role in progression of oral squamous cell carcinoma.

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