scholarly journals microRNA Utilize as a Potential Tool for Stress Tolerance in Plants

2021 ◽  
Author(s):  
Jyoti Rani
Keyword(s):  
Gene Expression ◽  
Heavy Metal ◽  
Stress Tolerance ◽  
Expression Profiling ◽  
Gene Expression Regulation ◽  
Nutrient Deficiency ◽  
Crop Plants ◽  
Environmental Condition ◽  
Control Gene ◽  
Potential Tool

This chapter describe the possibilities of MicroRNAs (miRNAs) in crop plants gene expression regulation in different metabolic pathways. Several current researches have shown different environmental stresses induce abnormal expression of miRNA, thus signifying that miRNAs may be an appropriate tool for genetical improvement in plant for stress tolerance. These miRNAs mainly control gene expression through translational inhibition. Generally, stress induce miRNAs-based inhibition of their target mRNAs, however, positive transcription factors accumulated and become more active after mRNA inhibition. Initially, researchers were mainly focused on miRNA identification, appropriate to specific or multiple environmental condition, expression profiling and recognize their roles in stress tolerance. Transformed miRNA expression studied in some plant species for better understanding of plant development and stress tolerance such as heavy metal, salinity, temperature, drought and nutrient deficiency. All these findings indicate that miRNAs act as a potential tool for genetic engineering and to enhance stress tolerance in crop plants.

Gene expression profiling to identify the toxicities and potentially relevant human disease outcomes associated with environmental heavy metal exposure

2017 ◽  
Vol 221 ◽  
pp. 64-74 ◽  
Author(s):  
Hesham M. Korashy ◽  
Ibraheem M. Attafi ◽  
Konrad S. Famulski ◽  
Saleh A. Bakheet ◽  
Mohammed M. Hafez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heavy Metal ◽  
Gene Expression Profiling ◽  
Human Disease ◽  
Expression Profiling ◽  
Metal Exposure ◽  
Heavy Metal Exposure ◽  
Disease Outcomes

scholarly journals Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish

Brain ◽  
2020 ◽  
Author(s):  
Juanjuan Luo ◽  
Pei Liu ◽  
Chunjiao Lu ◽  
Wanping Bian ◽  
Dongsheng Su ◽  
...  
Keyword(s):  
Gene Expression ◽  
Focal Adhesion ◽  
Expression Profiling ◽  
Signalling Pathways ◽  
Transgenic Zebrafish ◽  
High Grade ◽  
Human Gliomas ◽  
Bioinformatics Analyses ◽  
Single Deletion ◽  
Potential Tool

Abstract The molecular pathogenesis of glioblastoma indicates that RTK/Ras/PI3K, RB and TP53 pathways are critical for human gliomagenesis. Here, several transgenic zebrafish lines with single or multiple deletions of nf1, tp53 and rb1 in astrocytes, were established to genetically induce gliomagenesis in zebrafish. In the mutant with a single deletion, we found only the nf1 mutation low-efficiently induced tumour incidence, suggesting that the Nf1 pathway is critical for the initiation of gliomagenesis in zebrafish. Combination of mutations, nf1;tp53 and rb1;tp53 combined knockout fish, showed much higher tumour incidences, high-grade histology, increased invasiveness, and shortened survival time. Further bioinformatics analyses demonstrated the alterations in RTK/Ras/PI3K, cell cycle, and focal adhesion pathways, induced by abrogated nf1, tp53, or rb1, were probably the critical stepwise biological events for the initiation and development of gliomagenesis in zebrafish. Gene expression profiling and histological analyses showed the tumours derived from zebrafish have significant similarities to the subgroups of human gliomas. Furthermore, temozolomide treatment effectively suppressed gliomagenesis in these glioma zebrafish models, and the histological responses in temozolomide-treated zebrafish were similar to those observed in clinically treated glioma patients. Thus, our findings will offer a potential tool for genetically investigating gliomagenesis and screening potential targeted anti-tumour compounds for glioma treatment.

Metallothioneins and Phytochelatins: Role and Perspectives in Heavy Metal(loid)s Stress Tolerance in Crop Plants

2016 ◽  
pp. 237-264 ◽  
Author(s):  
Devesh Shukla ◽  
Prabodh K. Trivedi ◽  
Pravendra Nath ◽  
Narendra Tuteja
Keyword(s):  
Heavy Metal ◽  
Stress Tolerance ◽  
Crop Plants

Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling

2002 ◽  
Vol 69 ◽  
pp. 135-142 ◽  
Author(s):  
Elena M. Comelli ◽  
Margarida Amado ◽  
Steven R. Head ◽  
James C. Paulson
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Affymetrix Genechip ◽  
Microarray Technology ◽  
Gene Arrays ◽  
Glycosyltransferase Gene

The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.

278: Gene Expression Profiling of Prostatic Epithelial Cells in Two-Dimensional Versus Three-Dimensional Cultures

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Toshiyuki Tsunoda ◽  
Junichi Inocuchi ◽  
Darren Tyson ◽  
Seiji Naito ◽  
David K. Ornstein
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Three Dimensional ◽  
Two Dimensional

748: Gene Expression Profiling of Renal Medullary Carcinoma

2004 ◽  
Vol 171 (4S) ◽  
pp. 198-199 ◽  
Author(s):  
Ximing J. Yang ◽  
Jun Sugimura ◽  
Maria S. Tretiakova ◽  
Bin T. Teh
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Medullary Carcinoma ◽  
Renal Medullary Carcinoma
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