scholarly journals Plasmonic Nanopores: Optofluidic Separation of Nano-Bioparticles via Negative Depletion

2021 ◽  
Author(s):  
Xiangchao Zhu ◽  
Ahmet Cicek ◽  
Yixiang Li ◽  
Ahmet Ali Yanik
Keyword(s):  
Refractive Index ◽  
Chemical Composition ◽  
High Throughput ◽  
Light Transmission ◽  
Low Cost ◽  
Light Sources ◽  
Label Free ◽  
Incoherent Light ◽  
Drag Forces ◽  
On Chip

In this chapter, we review a novel “optofluidic” nanopore device enabling label-free sorting of nano-bioparticles [e.g., exosomes, viruses] based-on size or chemical composition. By employing a broadband objective-free light focusing mechanism through extraordinary light transmission effect, our plasmonic nanopore device eliminates sophisticated instrumentation requirements for precise alignment of optical scattering and fluidic drag forces, a fundamental shortcoming of the conventional optical chromatography techniques. Using concurrent optical gradient and radial fluidic drag forces, it achieves self-collimation of nano-bioparticles with inherently minimized spatial dispersion against the fluidic flow. This scheme enables size-based fractionation through negative depletion and refractive-index based separation of nano-bioparticles from similar size particles that have different chemical composition. Most remarkably, its small (4 μm × 4 μm) footprint facilitates on-chip, multiplexed, high-throughput nano-bioparticle sorting using low-cost incoherent light sources.

scholarly journals MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

2017 ◽  
Vol 22 (10) ◽  
pp. 1246-1252 ◽  
Author(s):  
Kishore Kumar Jagadeesan ◽  
Simon Ekström
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Web Application ◽  
Low Cost ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Label Free ◽  
Label Free Detection ◽  
R Shiny

Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4738 ◽  
Author(s):  
Mohamed Lemine Youba Diakité ◽  
Jerôme Champ ◽  
Stephanie Descroix ◽  
Laurent Malaquin ◽  
François Amblard ◽  
...  
Keyword(s):  
Long Range ◽  
Detection Method ◽  
Dna Detection ◽  
Low Cost ◽  
Label Free ◽  
Lab On Chip ◽  
Free Dna ◽  
Chip Format ◽  
Long Range Pcr ◽  
On Chip

scholarly journals A highly stable, nanotube-enhanced, CMOS-MEMS thermal emitter for mid-IR gas sensing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniel Popa ◽  
Richard Hopper ◽  
Syed Zeeshan Ali ◽  
Matthew Thomas Cole ◽  
Ye Fan ◽  
...  
Keyword(s):  
Gas Sensing ◽  
Low Cost ◽  
Complementary Metal Oxide Semiconductor ◽  
Oxide Semiconductor ◽  
Light Sources ◽  
Mir Spectroscopy ◽  
Sensing Applications ◽  
Cmos Mems ◽  
Extended Operation ◽  
On Chip

AbstractThe gas sensor market is growing fast, driven by many socioeconomic and industrial factors. Mid-infrared (MIR) gas sensors offer excellent performance for an increasing number of sensing applications in healthcare, smart homes, and the automotive sector. Having access to low-cost, miniaturized, energy efficient light sources is of critical importance for the monolithic integration of MIR sensors. Here, we present an on-chip broadband thermal MIR source fabricated by combining a complementary metal oxide semiconductor (CMOS) micro-hotplate with a dielectric-encapsulated carbon nanotube (CNT) blackbody layer. The micro-hotplate was used during fabrication as a micro-reactor to facilitate high temperature (>700 $$^{\circ }$$ ∘ C) growth of the CNT layer and also for post-growth thermal annealing. We demonstrate, for the first time, stable extended operation in air of devices with a dielectric-encapsulated CNT layer at heater temperatures above 600 $$^{\circ }$$ ∘ C. The demonstrated devices exhibit almost unitary emissivity across the entire MIR spectrum, offering an ideal solution for low-cost, highly-integrated MIR spectroscopy for the Internet of Things.

scholarly journals A highly stable, nanotube-enhanced, CMOS-MEMS thermal emitter for mid-IR gas sensing

2021 ◽  
Author(s):  
Daniel Popa ◽  
Richard Hopper ◽  
Syed Zeeshan Ali ◽  
Matthew Cole ◽  
Ye Fan ◽  
...  
Keyword(s):  
Gas Sensing ◽  
Low Cost ◽  
Complementary Metal Oxide Semiconductor ◽  
Oxide Semiconductor ◽  
Light Sources ◽  
Mir Spectroscopy ◽  
Sensing Applications ◽  
Cmos Mems ◽  
Extended Operation ◽  
On Chip

Abstract The gas sensor market is growing fast, driven by many socioeconomic and industrial factors. Mid-infrared (MIR) gas sensors offer excellent performance for an increasing number of sensing applications in healthcare, smart homes, and the automotive sector. Having access to low-cost, miniaturized, energy efficient light sources is of critical importance for the monolithic integration of MIR sensors. Here, we present an on-chip broadband thermal MIR source fabricated by combining a complementary metal oxide semiconductor (CMOS) micro-hotplate with a dielectric-encapsulated carbon nanotube (CNT) blackbody layer. The micro-hotplate was used during fabrication as a micro-reactor to facilitate high temperature (>700 • C) growth of the CNT layer and also for post-growth thermal annealing. We demonstrate, for the first time, stable extended operation in air of devices with a dielectric-encapsulated CNT layer at heater temperatures above 600 • C. The demonstrated devices exhibit almost unitary emissivity across the entire MIR spectrum, offering an ideal solution for low-cost, highly-integrated MIR spectroscopy for the Internet of Sensors.

scholarly journals Ultrafast-nonlinear ultraviolet pulse modulation in an AlInGaN polariton waveguide operating up to room temperature

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
D. M. Di Paola ◽  
P. M. Walker ◽  
R. P. A. Emmanuele ◽  
A. V. Yulin ◽  
J. Ciers ◽  
...  
Keyword(s):  
Refractive Index ◽  
Room Temperature ◽  
Light Sources ◽  
Optical Pulses ◽  
Pulse Modulation ◽  
Nonlinear Materials ◽  
Exciton Polaritons ◽  
Nonlinear Modulation ◽  
On Chip ◽  
New Generation

AbstractUltrafast nonlinear photonics enables a host of applications in advanced on-chip spectroscopy and information processing. These rely on a strong intensity dependent (nonlinear) refractive index capable of modulating optical pulses on sub-picosecond timescales and on length scales suitable for integrated photonics. Currently there is no platform that can provide this for the UV spectral range where broadband spectra generated by nonlinear modulation can pave the way to new on-chip ultrafast (bio-) chemical spectroscopy devices. We demonstrate the giant nonlinearity of UV hybrid light-matter states (exciton-polaritons) up to room temperature in an AlInGaN waveguide. We experimentally measure ultrafast nonlinear spectral broadening of UV pulses in a compact 100 μm long device and deduce a nonlinearity 1000 times that in common UV nonlinear materials and comparable to non-UV polariton devices. Our demonstration promises to underpin a new generation of integrated UV nonlinear light sources for advanced spectroscopy and measurement.

scholarly journals Use of LSPR Spectroscopy Biosensing for In Situ Identification of Arsenic from Bioleaching of Realgar by Acidithiobacillus ferrooxidans

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Ruixiang Xu ◽  
Wenbin Zhao ◽  
Shenghui Xu ◽  
Lei Yan ◽  
Zhengrong Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Response Speed ◽  
Localized Surface Plasmon ◽  
Enzyme Linked Immunosorbent Assay ◽  
Arsenic Content ◽  
Label Free ◽  
Monitoring Methods ◽  
Specific Selectivity

Localized surface plasmon resonance (LSPR) spectroscopy has received extensive attention as a new method in chemical and biological analysis that can be integrated with nanotechnology. As a new analytical method, LSPR possesses various advantages over the traditional bioanalysis method of enzyme-linked immunosorbent assay (ELISA), including a label-free procedure, low cost, high response speed, simple operation and structure, and ease of storage and transport. Additionally, in situ and high-throughput measurements can be achieved. This study aims to solve the problem of the lack of in situ and highly efficient monitoring methods for current realgar bioleaching processes. An LSPR chip is made to monitor the changes in arsenic content in the process of realgar bioleaching. A convenient, fast, sensitive, and high-throughput method of bioleaching process investigation based on the LSPR spectroscopic in situ monitoring technique is proposed. The LSPR chip provided a highly specific selectivity and a linear detection of arsenic content in the range of 1.0–100.0 μM with detection limit (LOD) 0.898 μM. The developed chip was applied to the quantification of realgar bioleaching sample with satisfactory results.

scholarly journals Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 345
Author(s):  
Kaiser Pärnamets ◽  
Tamas Pardy ◽  
Ants Koel ◽  
Toomas Rang ◽  
Ott Scheler ◽  
...  
Keyword(s):  
High Throughput ◽  
Focal Point ◽  
Low Cost ◽  
Fluorescent Microscopy ◽  
Detection Methods ◽  
Sensor Technology ◽  
Portable Devices ◽  
Light Sources ◽  
Microscopy Techniques ◽  
Massive Parallelization

High-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high flow rates, the droplets allow massive parallelization, further increasing the throughput of droplets. However, this novel methodology poses unique challenges related to commonly used fluorometry and fluorescent microscopy techniques. We review the optical sensor technology and light sources applicable to DMFC, as well as analyze the challenges and advantages of each option, primarily focusing on electronics. An analysis of low-cost and/or sufficiently compact systems that can be incorporated into portable devices is also presented.

scholarly journals Indistinguishable Photons from a Single Molecule under Pulsed Excitation

2021 ◽  
Vol 255 ◽  
pp. 06002
Author(s):  
Pietro Lombardi ◽  
Maja Colautti ◽  
Rocco Duquennoy ◽  
Ghulam Murtaza ◽  
Prosenjit Majumder ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Photon ◽  
Low Cost ◽  
Photon Emission ◽  
Light Sources ◽  
Single Photons ◽  
Quantum Communications ◽  
Ideal Quantum ◽  
On Chip ◽  
Key Resources

Quantum light sources are crucial for the future of quantum photonic technologies and, among them, single photons on-demand are key resources in quantum communications and information processing. Ideal quantum emitters providing indistinguishable photons in a clocked manner, negligible decoherence and spectral diffusion, and with potential for scalability are today still a major challenge. We report on photostable and indistinguishable single photon emission from dibenzoterrylene molecules isolated in anthracene nanocrystals (DBT:Ac NCs) at 3K. The visibility of two-photon interference is preserved even when they are separated more than thirty times the excited-state lifetime, or ten fluorescence cycles. One of the advantages of organic molecules is the low-cost mass production of nominally identical emitters, that also allow for on-chip integration. These aspects combined with high spectral stability and coherence make them promising for applications and future quantum technologies.

High-throughput acoustofluidic microchannels for single cell rotation

2021 ◽  
Author(s):  
Junwen Zhu ◽  
Qiqian Zhang ◽  
Fei Liang ◽  
Yongxiang Feng ◽  
Wenhui Wang
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Rotation Velocity ◽  
Microfluidic Channels ◽  
Lab On Chip ◽  
Dead End ◽  
Cell Rotation ◽  
Fabrication Procedure ◽  
On Chip ◽  
Highly Uniform

Abstract There is a growing desire for cell rotation in the field of biophysics, bioengineering and biomedicine. We herein present novel microfluidic channels for simultaneous high-throughput cell self-rotation using local circular streaming generated by ultrasonic wave excited bubble arrays. The bubble traps achieve high homogeneity of liquid-gas interface by setting capillary valves at the entrances of dead-end bubble trappers orthogonal to the main microchannel. In such a highly uniform bubble array, rotation at different fields of bubble-relevant vortices is considered equal and interconvertible. The device is compatible with cells of various size and retains manageable rotation velocity when actuated by signals of varying frequency and voltage. Experimental observations were confirmed consistent with theoretical estimation and numerical simulation. Comparing with the conventional approaches of cell rotation, our device has multiple merits such as high throughput, low cost and simple fabrication procedure, and high compatibility for lab-on-chip integration. Therefore, the platform holds a promise in cell observation, medicine development and biological detection.

Predictive Model for the Cell Passing Pressure in Deformation-Based CTC Chips

2014 ◽  
Author(s):  
Zhifeng Zhang ◽  
Jie Xu ◽  
Xiaolin Chen
Keyword(s):  
Surface Tension ◽  
High Throughput ◽  
Low Cost ◽  
Blood Stream ◽  
Ease Of Use ◽  
Threshold Pressure ◽  
Label Free ◽  
Future Design ◽  
Early Cancer Diagnosis ◽  
Cancer Metastases

Circulating tumor cells (CTCs) in blood stream have known to have cancer seeding effects and cause cancer metastases. Detection of CTCs plays an important role in early cancer diagnosis and treatment monitoring. A new technique of using microfluidic devices for CTC detection (CTC-chips) provides a promising solution. Among various CTC-chip designs, label-free chips based on cell size and deformability have the advantages of structural simplicity, stable performance, low cost and ease of use. In deformation-based CTC-chips, the threshold pressure required to pass CTCs through the chips is a key parameter of informing the CTC filtering capability and device sensitivity. Most existing models for the threshold pressure in deformation-based CTC-chips are based on quasi-static Young-Laplace surface tension model. However, for modeling high throughput CTC-chips, dynamic terms such as pressure drop in the surrounding flow should be taken into consideration. In the present work, we consider liquid-liquid (cell-medium) interfacial tension along with the effect of fluid flow in the threshold pressure prediction, and propose a second order relationship between the threshold pressure and the reciprocal of hydraulic diameter of the filter channel. Based on the new equation, the effect of parameters such as surface tension coefficient, viscosity, velocity and channel roundness are studied. This predicative model can be a valuable tool to aid future design of high throughput CTC-chips.

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