scholarly journals Atlas of Age- and Tissue-Specific DNA Methylation during Early Development of Barley (Hordeum vulgare)

2020 ◽  
Author(s):  
Moumouni Konate ◽  
Mike J. Wilkinson ◽  
Benjamin T. Mayne ◽  
Eileen S. Scott ◽  
Bettina Berger ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Hordeum Vulgare ◽  
Early Development ◽  
Tissue Specific

scholarly journals Atlas of Age- and Tissue-specific DNA Methylation during Early Development of Barley (Hordeum vulgare)

2018 ◽  
Author(s):  
Moumouni Konate ◽  
Michael J. Wilkinson ◽  
Banjamin Mayne ◽  
Eileen Scott ◽  
Bettina Berger ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Hordeum Vulgare ◽  
Organ Function ◽  
Tissue Specific ◽  
Organ Differentiation ◽  
Differentiated Cells ◽  
Differentially Methylated Genes ◽  
Organ Specific ◽  
Development And Differentiation ◽  
Methylation Patterns

The barley (Hordeum vulgare) genome comprises over 32,000 genes, with differentiated cells expressing only a subset of genes; the remainder being silent. Mechanisms by which tissue-specific genes are regulated are not entirely understood, although DNA methylation is likely to be involved. DNA methylation patterns are not static during plant development, but it is still unclear whether different organs possess distinct methylation profiles. Methylation-sensitive GBS was used to generate DNA methylation profiles for roots, leaf-blades and leaf-sheaths from five barley varieties, using seedlings at the three-leaf stage. Differentially Methylated Markers (DMMs) were characterised by pairwise comparisons of roots, leaf-blades and leaf-sheaths of three different ages. While very many DMMs were found between roots and leaf parts, only a few existed between leaf-blades and leaf-sheaths, with differences decreasing with leaf rank. Organ-specific DMMs appeared to target mainly repeat regions, implying that organ differentiation partially relies on the spreading of DNA methylation from repeats to promoters of adjacent genes. Furthermore, the biological functions of differentially methylated genes in the different organs correlated with functional specialisation. Our results indicate that different organs do possess diagnostic methylation profiles and suggest that DNA methylation is important for both tissue development and differentiation and organ function.

Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs

2021 ◽  
Author(s):  
Xiaotong Wu ◽  
Hongmei Zhang ◽  
Bingjie Zhang ◽  
Yu Zhang ◽  
Qiuyan Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Early Development ◽  
Epigenetic Reprogramming ◽  
Adult Tissue ◽  
Dna Hypermethylation ◽  
Tissue Specific ◽  
Early Embryos

Drastic epigenetic reprogramming is essential to convert terminally-differentiated gametes to totipotent embryos. However, it remains puzzling why post-fertilization global DNA reprogramming occurs only in mammals but not in non-mammalian vertebrates. In zebrafish, global methylome inheritance is however accompanied by sweeping enhancer "dememorization" as they become fully methylated. By depleting maternal dnmt1 using oocyte microinjection in situ, we eliminated DNA methylation in zebrafish early embryos, which died around gastrulation with severe differentiation defects. Strikingly, methylation deficiency leads to extensive derepression of adult tissue-specific genes and CG-rich enhancers, which acquire ectopic TF binding and, unexpectedly, H3K4me3. By contrast, embryonic enhancers are generally CG-poor and evade DNA methylation repression. Hence, global DNA hypermethylation inheritance coupled with enhancer dememorization installs an epigenetic gate that safeguards embryonic programs and ensures temporally ordered gene expression. We propose that "enhancer dememorization" underlies and unifies distinct epigenetic reprogramming modes in early development between mammals and non-mammals.

Early Development and Tissue-Specific Patterns of Insulin Binding in Chick Embryo*

Endocrinology ◽  
1984 ◽  
Vol 115 (4) ◽  
pp. 1315-1323 ◽  
Author(s):  
S. ANNE HENDRICKS ◽  
FLORA DE PABLO ◽  
JESSE ROTH
Keyword(s):  
Chick Embryo ◽  
Early Development ◽  
Insulin Binding ◽  
Tissue Specific

scholarly journals Non-canonical RNA-directed DNA methylation participates in maternal and environmental control of seed dormancy

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Mayumi Iwasaki ◽  
Lena Hyvärinen ◽  
Urszula Piskurewicz ◽  
Luis Lopez-Molina
Keyword(s):  
Dna Methylation ◽  
Seed Dormancy ◽  
Environmental Control ◽  
Negative Regulator ◽  
Paternal Allele ◽  
Seed Progeny ◽  
Tissue Specific ◽  
Parental Allele ◽  
Allele Expression ◽  
Premature Germination

Seed dormancy is an adaptive trait preventing premature germination out of season. In a previous report (Piskurewicz et al., 2016) we showed that dormancy levels are maternally inherited through the preferential maternal allele expression in the seed endosperm of ALLANTOINASE (ALN), a negative regulator of dormancy. Here we show that suppression of ALN paternal allele expression is imposed by non-canonical RNA-directed DNA methylation (RdDM) of the paternal ALN allele promoter. Dormancy levels are further enhanced by cold during seed development. We show that DNA methylation of the ALN promoter is stimulated by cold in a tissue-specific manner through non-canonical RdDM, involving RDR6 and AGO6. This leads to suppression of ALN expression and further promotion of seed dormancy. Our results suggest that tissue-specific and cold-induced RdDM is superimposed to parental allele imprints to deposit in the seed progeny a transient memory of environmental conditions experienced by the mother plant.

scholarly journals DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific

2000 ◽  
Vol 32 (4) ◽  
pp. 397-404 ◽  
Author(s):  
Luis Torres ◽  
Gerardo López-Rodas ◽  
M.Ujue Latasa ◽  
M.Victoria Carretero ◽  
Abdelhalim Boukaba ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Histone Acetylation ◽  
Methionine Adenosyltransferase ◽  
Tissue Specific

scholarly journals Age-related DNA methylation changes are tissue-specific with ELOVL2 promoter methylation as exception

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Roderick C. Slieker ◽  
Caroline L. Relton ◽  
Tom R. Gaunt ◽  
P. Eline Slagboom ◽  
Bastiaan T. Heijmans
Keyword(s):  
Dna Methylation ◽  
Promoter Methylation ◽  
Tissue Specific ◽  
Age Related
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