scholarly journals Subcellular Localization of Glutathione Peroxidase, Change in Glutathione System during Ageing and Effects on Cardiometabolic Risks and Associated Diseases

2020 ◽  
Author(s):  
Théophile Mbemba Fundu ◽  
Paulin Mutwale Kapepula ◽  
Justin Mboloko Esimo ◽  
José Remacle ◽  
Nadege Kabamba Ngombe
Keyword(s):  
Glutathione Peroxidase ◽  
Subcellular Localization ◽  
Glutathione System ◽  
Associated Diseases ◽  
Cardiometabolic Risks

Effects of hypoxia and reoxygenation on lung glutathione system

1990 ◽  
Vol 259 (2) ◽  
pp. H518-H524 ◽  
Author(s):  
R. M. Jackson ◽  
C. F. Veal
Keyword(s):  
Glutathione Peroxidase ◽  
Lung Tissue ◽  
Neutrophil Infiltration ◽  
Lavage Fluid ◽  
Glutathione System ◽  
Glutathione Disulfide ◽  
Lung Collapse ◽  
Total Glutathione ◽  
Reexpansion Pulmonary Edema ◽  
The Right

Reexpansion pulmonary edema (RPE) parallels reperfusion (reoxygenation) injuries in other organs in that hypoxic and hypoperfused lung tissue develops increased vascular permeability and neutrophil infiltration after reexpansion. This study investigated the lung cellular glutathione system during hypoxia (produced by lung collapse) and after reoxygenation (produced by reexpansion). Two separate groups of rabbits were studied to determine effects of lung hypoxia-reoxygenation on 1) lung glutathione peroxidase and reductase enzyme activities and 2) lung tissue, plasma, and alveolar lavage fluid total (reduced glutathione plus glutathione disulfide) and oxidized glutathione. Neither lung collapse for 3-7 days nor reexpansion for 2 h after 7 days of collapse affected glutathione peroxidase [controls, 0.36 +/- 0.04 (left), 0.38 +/- 0.03 U/mg DNA (right)] or reductase [controls, 0.12 +/- 0.01 (left), 0.14 +/- 0.01 U/mg DNA (right)] activities. The concentration of glutathione disulfide increased markedly in right alveolar lavage fluid, but not in plasma, after right lung reexpansion. Right lung total glutathione decreased significantly (-19%) after 7 days of collapse. After right lung reexpansion, both left (-65%) and right (-68%) lung total glutathione decreased significantly. The percent of total glutathione present in the oxidized form increased significantly in both left (to 15.5 +/- 4.0% of total) and right (to 18.7 +/- 6.3% of total) lungs after reexpansion of the right lung. These data indicate that lung tissue hypoxia, produced by unilateral lung collapse, was associated with a unilateral decrease in lung total glutathione content. Right lung reoxygenation, due to rapid reexpansion, caused a bilateral decrease in lung total glutathione content and an increase in right lung and alveolar lavage fluid glutathione disulfide concentration.

scholarly journals Glutathione system in the blood serum of rats in the dynamics of full-thickness wounds and with the influence of new pharmacological composition containing melanine

2017 ◽  
Vol 22 (1) ◽  
pp. 5-8
Author(s):  
O. Taburets ◽  
K. Dvorshenko ◽  
M. Tymoshenko ◽  
V. Vereschaka ◽  
T. Beregova ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Glutathione Transferase ◽  
Reductase Activity ◽  
Transferase Activity ◽  
Full Thickness ◽  
Glutathione System ◽  
Glutathione Reductase Activity ◽  
Activity Of Enzymes ◽  
Full Thickness Wound

The activity of enzymes of the glutathione link of the antioxidant system in rats with experimental full-thickness wound has been studied. It is established that in the serum of rats under the experimental model of the cut wound the level of recovered glutathione and glutathione reductase activity decreased, glutathione peroxidase and glutathione transferase activity increased. All investigated parameters normalized in dynamics after treatment with melanin.

scholarly journals Disease Associated Variations in Glutathione Peroxidase‐1 Affect Its Subcellular Localization and Function

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Dede Ekoue ◽  
Soumen Bera ◽  
Frank Weinberg ◽  
Kristine Fricano ◽  
Mao Mao ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Subcellular Localization ◽  
Glutathione Peroxidase 1 ◽  
And Function

scholarly journals Glutathione system in rat blood serum under the conditions of carrageenan-induced joint inflammation and chondroitin sulfate prophylactic injection

2021 ◽  
Vol 85 (2) ◽  
pp. 32-37
Author(s):  
K. Dvorshchenko ◽  
O. Korotkiy ◽  
D. Grebinyk ◽  
Ye. Torgalo
Keyword(s):  
Glutathione Peroxidase ◽  
Chondroitin Sulfate ◽  
Peroxidase Activity ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Joint Inflammation ◽  
Transferase Activity ◽  
Glutathione System ◽  
Nacl Solution ◽  
Glutathione Peroxidase Activity

The goal of this work was the research of chondroitin sulfate prophylactic action on the glutathione system state in rat blood serum during acute joint inflammation. The research was performed on white non-linear pubertal rat males weighting 160-240 grams in compliance to all general ethical principles of animal research. All animals were divided to four experimental groups. The first group – the control: the animals were injected with 0,1 ml of 0,9 % NaCl solution in a right hind leg. The second group – rats were subjected to daily intramuscular injections of 3 mg/kg of chondroitin sulfate in the theurapeutic dose during 28 days. The third group – the animals were subjected to daily doses of 0,1 ml of 0,9 % NaCl solution injected in right hind extremities and starting from 29th day the acute joint inflammation was modelled (the animals were subjected to 0,1 ml of 1% of сarrageenan intramuscular injection in right hind extremities. The fourth group – rats were receiving intramuscular injections of therapeutic dose of 3 mg/kg of chondroitin sulfate for 28 days, and after that the acute joint inflammation was modelled starting from 29th day. 40 animals in all were taking part in the experimental research. The glutathione peroxidase activity was assessed judging from the decrease in GSH amount in the reaction with Ellman reagent. The glutathione peroxidase activity was estimated by the decrease in probe optical density owing to NADPH oxidation. Glutathione transferase activity was estimated by the speed of the conjugate formation between GSH and 1-chloro-2,4-dinitrobemzene. The amount of reduced glutathione was estimated spectrophotometrically using orthophthalic aldehyde. It was established that during the carrageenan-induced inflammation the glutathione peroxidase, glutathione reductase and glutathione reductase activity were reduced, a well as the amount of reduced glutathione, whereas the glutathione transferase activity was increased in comparison to the control. It was shown that under the prophylactic injection of the chondroitin sulfate based preparate to animals with acute joint inflammation the aforementioned parameters were partially stabilized.

scholarly journals OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES

2014 ◽  
Vol 13 (3) ◽  
pp. 84-90 ◽  
Author(s):  
Ye. V. Shakhristova ◽  
Ye. A. Stepovaya ◽  
V. V. Ivanov ◽  
O. L. Nosareva ◽  
N. V. Ryazantseva ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glutathione Peroxidase ◽  
Alloxan Diabetes ◽  
Antioxidant Defense ◽  
Oxidative Modification ◽  
Glutathione System ◽  
Glutathione Peroxidase Activity ◽  
Oxidative Modification Of Proteins ◽  
Carbonyl Derivatives ◽  
Derivatives Of

Currently, diabetes ranks third in relation to medical and social significance after cardiovascular diseases and cancer and is the leading cause of blindness; it greatly increases the risk of myocardial infarction, coronary heart disease, nephropathy and hypertension in patients with this disorder; therefore clinical and experimental studies aimed at investigation of diabetes emergence and development mechanisms are urgent.The aim of the study was to investigate the status of oxidative modification of proteins and glutathionedependent antioxidant defense system in adipocytes of rats with alloxan diabetes under conditions of oxidative stress.Material and methods. Development of type 1 diabetes was induced in rats by alloxan administration (90 mg/kg of body mass). Adipocytes were obtained from epididymal adipose tissue of rats. The level of carbonyl derivatives of proteins, oxidized tryptophan, bityrosine, general, reduced, oxygenated and protein-bound glutathione, as well as glutathione peroxidase activity in adipocytes of rats was determined.Results. In adipocytes of rats with alloxan diabetes, concentration of carbonyl derivatives of proteins, bityrosine and oxidized tryptophan increased on the background of redox-potential of glutathione system and glutathione peroxidase activity decrease.Conclusion. The obtained data indicate the activation of free-radical oxidation of proteins and reduction of antioxidant defense under conditions of oxidative stress in the adipose tissue of rats with alloxan diabetes; this process plays an important role in pathogenesis of diabetes and its complications development.

scholarly journals АНТИОКСИДАНТНИЙ ЗАХИСТ ТА ПЕРОКИСНЕ ОКИСНЕННЯ ЛІПІДІВ ОРГАНІЗМУ ВИСОКОТІЛЬНИХ КОРІВ ПРИ ПРОФІЛАКТИЦІ САЛЬМОНЕЛЬОЗУ ЗА ВПЛИВУ НАНОПРЕПАРАТУ ГЕРМАКАПУ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 112-116
Author(s):  
P.Y. Lavriv
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Antioxidant Defense ◽  
Reductase Activity ◽  
Lipid Body ◽  
Antioxidant Defense System ◽  
Glutathione System ◽  
Defense System ◽  
Glutathione Reductase Activity ◽  
Radical Processes

The article  deals with the results of experimental research data of relationship between the antioxidant defense system and lipid body peroxidation of high calves cows  under  the influence of nanpreparation Germakap conducted  with simultaneous vaccination with inactivated formol vaccination with repeated  in two weeks later  at the same doses as their  stability and immunity to Salmonella. It was found the likely increase in activity of glutathione peroxidase and glutathione reductase and at the same time, reduction of malon dialdehyde and hydroperoxides lipids and superoxidimutase. These changes in animals  body occur due to complex components adaptive nan preparation Germakap that lead to the normalization of metabolic and free radical processes in animals.  However, the increase in the catalytic activity of glutathione peroxidase and glutathione reductase activity in plasma of high calves cows  from research group can be explained by increasing intensity of synthesis in which these enzymes by introducing nan preparation Germakap closely associated with the regeneration of glutathione in the cell, and also the activity of glutathione peroxidase.  Through interaction with restoration of glutathione and glutathione peroxidase, glutathione reductase it was formed glutathione system that protects cells from stress peroxidation

scholarly journals The glutathione system in bone tissue under the action of copper-zinc ore components and antioxidants administration

2021 ◽  
Vol 102 (2) ◽  
pp. 199-205
Author(s):  
G R Kuramshina ◽  
F Kh Kamilov
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Bone Tissue ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Antioxidant Vitamin ◽  
Glutathione System ◽  
Thiol Groups ◽  
Chronic Intoxication ◽  
Copper Zinc

Aim. To study changes in the glutathione system in bone tissue during chronic intoxication with elements contained in copper-zinc pyrite ore and antioxidant vitamin administration. Methods. 36 mature male white rats were divided into three groups (control, comparison, experimental). The rats of the experimental and comparison groups received intragastrically copper-zinc pyrite ore powder in a 2% starch solution as a suspension at a dosage of 60 mg/100 g bodyweight daily for three months. During the last month, the experimental group received an antioxidant vitamin preparation (the complex of vitamins with a trace element) containing -tocopherol, -carotene, ascorbic acid and selenium. The content of reduced glutathione, free thiol groups in proteins, the activity of glutathione peroxidase, glutathione transferase, glutathione reductase, gamma-glutamyl transferase, and glucose-6-phosphate dehydrogenase were determined in homogenates derived from femoral epiphysis. The statistical analysis of the results was performed using Statistica 6.0 software. The median (Me) and percentiles (Q1 and Q2) were calculated, a non-parametric MannWhitney U test was carried out to compare study groups. Results. Chronic intoxication with elements contained in copper-zinc pyrite ore causes impairment of the glutathione system in bone tissue. Intoxicated rats showed a decrease in the reduced glutathione content to 71.9% (р=0.014) and free sulfhydryl groups of proteins to 77.8% (р=0.0143), inhibition of glutathione-dependent antioxidant enzymes activities, and disruption of the glutathione reduction system in tissues, compared to the control group. Antioxidant vitamin administration increased the levels of reduced glutathione and free thiol groups of proteins, activated the enzymes involved in the glutathione system: the reduced glutathione content increased to 94.8% (p=0.2132), glutathione peroxidase activity to 85.7% (p=0.0432), glutathione transferase up to 94.3% (p=0.5251), glutathione reductase up to 86.1% (p=0.0442) compared to the control group. Conclusion. Chronic intoxication with metals contained in copper-zinc pyrite ore leads to decreasing the content of reduced glutathione and free thiol groups of proteins in bones along with reducing glutathione reductase and glucose-6-phosphate dehydrogenase activities, inhibition of glutathione peroxidase and glutathione transferase; an antioxidant vitamin administration increases the activity of glutathione reduction enzymes in bone tissue, the content of reduced glutathione and free sulfhydryl groups of proteins, the activities of glutathione peroxidase and glutathione transferase.

scholarly journals The rat blood glutathione system under obesity development with the compatible consumption of the kidney bean (Phaseolus vulgaris) pods extract

2018 ◽  
Vol 76 (2) ◽  
pp. 37-42
Author(s):  
A. Yurchenko ◽  
D. Krenytska ◽  
M. Tymoshenko
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Glutathione Transferase ◽  
Antioxidant Defense System ◽  
Kidney Bean ◽  
Glutathione System ◽  
Activity Increase ◽  
Calorie Diet ◽  
High Calorie Diet ◽  
High Calorie

The state of the glutathione-dependent link of the antioxidant blood system of rats under obesity development and with the compatible consumption of the kidney bean (P. vulgaris) pods extract was studied. Glutathione system forms functional basis of organism antioxidant defense system and disturbances in its work can lead to a number of serious cytotoxic and destructive lesions. Glutathione system constituent elements has its own glutathione and enzymes, which catalyze the reaction of its reverse transformation. These include glutathione peroxidase, glutathione transferase, and glutathione reductase. It is known that the water kidney bean (P. vulgaris) pods extract has hypoglycemic properties. However, complex studies of the polyfunctional action of this extract in obesity are absent. The obtained results indicate a decrease in the overall potential of the glutathione system in animals on a high-calorie diet. It has been established that glutathione peroxidase activity (GP) decreases in the serum of rats under experimental obesity, also glutathione transferase (GT) and glutathione reductase (GR) activity increase compared to the corresponding values in control animals. Our results demonstrated the concentration decrease of recovered glutathione (GSH) under consumption of high-calorie diet compared to the control. The findings after 6-th weeks of kidney bean (P. vulgaris) pods extract consumption indicate the positive modulating effect of this extract on the content of GSH and the activity of GP, GT and GR in rats with the obesity development. Probably, the consumption of this extract leads to attraction of the investigated components of the antioxidant system to the reactions of neutralization of toxic free radical compounds that were formed against the background of the development of obesity. Further studies may serve as the basis for the development of new therapeutic or medicinal preparations that based on the kidney bean (P. vulgaris) pods extract, because of still no consensus on the treatment of obesity.

scholarly journals The influence of melatonin on the state of the glutathione system of the rat liver under the conditions of subacute alcoholic intoxication

2014 ◽  
Vol 18 (3 (71)) ◽  
Author(s):  
N. V. Davydova
Keyword(s):  
Glutathione Peroxidase ◽  
Oral Administration ◽  
Rat Liver ◽  
Peroxidase Activity ◽  
Reduced Glutathione ◽  
Alcohol Intoxication ◽  
Transferase Activity ◽  
Glutathione System ◽  
Glutathione Peroxidase Activity ◽  
Glutathione S Transferase

The investigations of certain parameters of glutathione system in the liver of rats under the conditions of subacute alcohol intoxication revealed a decreased content of reduced glutathione and glutathione peroxidase activity as well as an activation of glutathione-S-transferase activity. An oral administration of “Vita-melatonin” against a background of subacute alcohol intoxication in a dose 5 mg/kg during 10 days prevented the changes of the parameters under study.

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