scholarly journals A New Mouse Model of Aortic Aneurysm Induced by Deoxycorticosterone Acetate or Aldosterone in the Presence of High Salt

2020 ◽  
Author(s):  
Ming C. Gong ◽  
Shu Liu ◽  
Zhenheng Guo
Keyword(s):  
Aortic Aneurysm ◽  
Mouse Model ◽  
High Salt ◽  
Deoxycorticosterone Acetate

scholarly journals Ultrastructural Imaging of Collagen Fibrils in Mouse Model of Abdominal Aortic Aneurysm

2016 ◽  
Vol 22 (S3) ◽  
pp. 1196-1197 ◽  
Author(s):  
Jeffrey R Tonniges ◽  
Ben Albert ◽  
Edward Calomeni ◽  
Chetan Hans ◽  
Gunjan Agarwal
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Mouse Model ◽  
Collagen Fibrils ◽  
Abdominal Aortic

Abstract 356: Serum Amyloid A Augments Aortic Aneurysm Formation Induced by Mineralocorticoid Receptor Agonists in the Presence of High Salt

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Nancy R Webb ◽  
Joanne M Wroblewski ◽  
Jenny Lutshumba ◽  
Maria C De Beer ◽  
Vicky P Noffsinger ◽  
...  
Keyword(s):  
Aortic Aneurysm ◽  
Acute Phase ◽  
Mineralocorticoid Receptor ◽  
Serum Amyloid A ◽  
The United States ◽  
High Salt ◽  
Serum Amyloid ◽  
Receptor Agonists ◽  
Amyloid A ◽  
Aneurysm Formation

Objectives: The annual mortality in the United States from ruptured aortic aneurysms is ~15000. Therapeutic interventions that prevent AAA progression and rupture remain to be identified. In humans, plasma concentrations of the acute phase reactant serum amyloid A (SAA) correlates with aortic dimensions before aneurysm formation. We have shown that endogenous SAA augments AAA in the well-established angiotensin II (AngII) infusion mouse model (unpublished data). Here we investigated whether endogenous SAA impacts aneurysm formation induced by deoxycorticosterone acetate (DOCA), a mineralocorticoid receptor agonist, in the presence of high salt. Approach and results: DOCA pellets (50mg, 21 day release) were implanted subcutaneously in the lateral dorsal region of 8-month old male C57BL/6 (SAAWT) mice and C57BL/6 mice lacking both acute phase SAA isoforms, SAA1.1 and SAA2.1 (SAAKO). The mice were also provided drinking water containing 0.9% NaCl and 0.2% KCl for 21 days (n = 7-8). As expected, DOCA + salt resulted in significantly increased systolic blood pressure, which was not affected by the absence of SAA. Unexpectedly SAAKO mice displayed a reduced urine output, accompanied by a reduced water intake. Plasma sodium and potassium concentrations in SAAWT and SAAKO mice were similar after treatment. The maximal luminal diameter of the abdominal aorta, as determined by ultrasound, was significantly lower in SAAKO mice compared to SAAWT mice after a 3-week DOCA + salt regime. Aneurysm incidence, determined by ultrasound and ex vivo analyses, was 67% for SAAWT mice and 25 % for SAAKO mice. Notably, plasma SAA was markedly increased in the SAAWT mice that formed aneurysms compared to those that did not. In SAAWT mice, immunohistochemical staining and in situ zymography identified SAA in aneurysmal aortic tissue, but not control aortas, that co-localized to regions of enhanced matrix metalloproteinase (MMP) activity, suggesting a role for SAA in MMP activation. Conclusions: We conclude that endogenous SAA augments aortic aneurysm formation induced by mineralocorticoid receptor agonists in the presence of high salt. Thus, SAA contributes to pathological processes leading to aortic aneurysm in two robust and mechanistically distinct animal models.

Localized Administration of Doxycycline Suppresses Aortic Dilatation in an Experimental Mouse Model of Abdominal Aortic Aneurysm

2006 ◽  
Vol 20 (2) ◽  
pp. 228-236 ◽  
Author(s):  
Michel A. Bartoli ◽  
Federico E. Parodi ◽  
Jack Chu ◽  
Monica B. Pagano ◽  
Dongli Mao ◽  
...  
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Mouse Model ◽  
Aortic Dilatation ◽  
Abdominal Aortic ◽  
Experimental Mouse Model ◽  
Experimental Mouse

scholarly journals Kallikrein‐1 Blockade Inhibits Aortic Expansion in a Mouse Model and Reduces Prostaglandin E2 Secretion From Human Aortic Aneurysm Explants

2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Corey S. Moran ◽  
Erik Biros ◽  
Smriti M. Krishna ◽  
Susan K. Morton ◽  
Daniel J. Sexton ◽  
...  
Keyword(s):  
Aortic Aneurysm ◽  
Mouse Model ◽  
Matrix Metalloproteinase ◽  
Cyclooxygenase 2 ◽  
Matrix Metalloproteinase 2 ◽  
Prostaglandin E ◽  
Blocking Antibody ◽  
Active Matrix ◽  
Suprarenal Aorta ◽  
Metalloproteinase 9

Background Abdominal aortic aneurysm (AAA) is an important cause of mortality in older adults. The kinin B2 receptor agonist, bradykinin, has been implicated in AAA pathogenesis through promoting inflammation. Bradykinin is generated from high‐ and low‐molecular‐weight kininogen by the serine protease kallikrein‐1. The aims of this study were first to examine the effect of neutralizing kallikrein‐1 on AAA development in a mouse model and second to test how blocking kallikrein‐1 affected cyclooxygenase‐2 and prostaglandin E 2 in human AAA explants. Methods and Results Neutralization of kallikrein‐1 in apolipoprotein E‐deficient ( ApoE −/− ) mice via administration of a blocking antibody inhibited suprarenal aorta expansion in response to angiotensin (Ang) II infusion. Kallikrein‐1 neutralization decreased suprarenal aorta concentrations of bradykinin and prostaglandin E 2 and reduced cyclooxygenase‐2 activity. Kallikrein‐1 neutralization also decreased protein kinase B and extracellular signal‐regulated kinase 1/2 phosphorylation and reduced levels of active matrix metalloproteinase 2 and matrix metalloproteinase 9. Kallikrein‐1 blocking antibody reduced levels of cyclooxygenase‐2 and secretion of prostaglandin E 2 and active matrix metalloproteinase 2 and matrix metalloproteinase 9 from human AAA explants and vascular smooth muscle cells exposed to activated neutrophils. Conclusions These findings suggest that kallikrein‐1 neutralization could be a treatment target for AAA.

scholarly journals New Mouse Model of Abdominal Aortic Aneurysm

2017 ◽  
Vol 37 (11) ◽  
pp. 1990-1993 ◽  
Author(s):  
Stoyan N. Angelov ◽  
Jay Zhu ◽  
David A. Dichek
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Mouse Model ◽  
Abdominal Aortic
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