scholarly journals Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology

2019 ◽  
Author(s):  
Cláudia Jassica Gonçalves Moreno ◽  
Johny Wysllas de Freitas Oliveira ◽  
Joice Castelo Branco ◽  
Laura Araújo ◽  
Aline Maria Queiroz ◽  
...  
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi

Some observations on the effect of Ro 7-1051 on Trypanosoma cruzi, particularly in cell culture

1977 ◽  
Vol 33 (9) ◽  
pp. 1201-1202 ◽  
Author(s):  
S. Yoneda ◽  
E. Kimura ◽  
Beatriz Ribas Castellani
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi

scholarly journals Murine virus contaminant of Trypanosoma cruzi experimental infection

1994 ◽  
Vol 36 (5) ◽  
pp. 423-431 ◽  
Author(s):  
Humberto de A. Rangel ◽  
Liana Verinaud ◽  
Irineu J. B. Camargo ◽  
Rovilson Gilioli ◽  
Julia K. Sakurada
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi ◽  
Experimental Infection ◽  
Host Response ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Survival Times ◽  
Mean Survival Time ◽  
The Mean ◽  
Mouse Antibody

The possibility that some virus contaminants could be altering host response to Trypanosoma cruzi experimental infection was investigated. Data obtained showed that CBA/J mice infected with stocks of parasite maintained in mice (Y1UEC) presented higher level of parasitemia and shorter survival times than those infected with a stock (Y1TC) which was also maintained in mice but had been previously passaged in cell culture. Mouse antibody production tests, performed with the filtered plasma of mice infected with Y1UEC, indicated the presence of mouse hepatitis virus (MHV) while no virus was detected when testing the plasma of Y1TC infected mice. Filtered plasma of Y1EUC infected mice was shown to contain a factor able to enhance the level of parasitemia and to reduce the mean survival time of mice challenged with 10(5) Y1TC. This factor, that could be serially passaged to naïve mice was shown to be a coronavirus by neutralization tests.

scholarly journals Neue Parameter für die Wirkstofftestung gegen Trypanosoma cruzi

BIOspektrum ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 168-170
Author(s):  
Anna Fesser ◽  
Marcel Kaiser ◽  
Pascal Mäser
Keyword(s):  
Cell Culture ◽  
Latin America ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
New Drugs ◽  
High Content Imaging ◽  
Chronic Stage ◽  
Triatomine Bugs ◽  
And Migration

AbstractChagas disease is a zoonosis caused by Trypanosoma cruzi and transmitted by triatomine bugs. Autochthonous to Latin America, Chagas disease has spread globally through travel and migration. New drugs are needed urgently, in particular drugs that cure the chronic stage. This is where high-content imaging makes a key contribution: assays with fluorescent parasites in cell culture allow to determine pharmacodynamic parameters and to better assess the antichagasic potential of new molecules.

scholarly journals Golgi UDP-GlcNAc:PolypeptideO-α-N-Acetyl-d-Glucosaminyltransferase 2 (TcOGNT2) Regulates Trypomastigote Production and Function in Trypanosoma cruzi

2014 ◽  
Vol 13 (10) ◽  
pp. 1312-1327 ◽  
Author(s):  
Carolina M. Koeller ◽  
Hanke van der Wel ◽  
Christa L. Feasley ◽  
Fernanda Abreu ◽  
Juliana Dutra Barbosa da Rocha ◽  
...  
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi ◽  
Low Frequency ◽  
Protozoan Parasite ◽  
Infection Frequency ◽  
Content Type ◽  
Protein Levels ◽  
Life Cycle Stages ◽  
Altered Cell ◽  
And Function

ABSTRACTAll life cycle stages of the protozoan parasiteTrypanosoma cruziare enveloped by mucin-like glycoproteins which, despite major changes in their polypeptide cores, are extensively and similarlyO-glycosylated.O-Glycan biosynthesis is initiated by the addition of αGlcNAc to Thr in a reaction catalyzed by Golgi UDP-GlcNAc:polypeptideO-α-N-acetyl-d-glucosaminyltransferases (ppαGlcNAcTs), which are encoded byTcOGNT1andTcOGNT2. We now directly show that TcOGNT2 is associated with the Golgi apparatus of the epimastigote stage and is markedly downregulated in both differentiated metacyclic trypomastigotes (MCTs) and cell culture-derived trypomastigotes (TCTs). The significance of downregulation was examined by forced continued expression of TcOGNT2, which resulted in a substantial increase of TcOGNT2 protein levels but only modestly increased ppαGlcNAcT activity in extracts and altered cell surface glycosylation in TCTs. Constitutive TcOGNT2 overexpression had no discernible effect on proliferating epimastigotes but negatively affected production of both types of trypomastigotes. MCTs differentiated from epimastigotes at a low frequency, though they were apparently normal based on morphological and biochemical criteria. However, these MCTs exhibited an impaired ability to produce amastigotes and TCTs in cell culture monolayers, most likely due to a reduced infection frequency. Remarkably, inhibition of MCT production did not depend on TcOGNT2 catalytic activity, whereas TCT production was inhibited only by active TcOGNT2. These findings indicate that TcOGNT2 downregulation is important for proper differentiation of MCTs and functioning of TCTs and that TcOGNT2 regulates these functions by using both catalytic and noncatalytic mechanisms.

scholarly journals Differences in the antigenic profile of bloodstream and cell culture derived trypomastigotes of Trypanosoma cruzi

1989 ◽  
Vol 31 (3) ◽  
pp. 146-150
Author(s):  
Ana M. M. Da Silva ◽  
Cláudia I. Brodskyn ◽  
Harumi A. Takehara ◽  
I. Mota
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi ◽  
Comparative Study ◽  
Infected Blood ◽  
Chagasic Patient ◽  
Physiological Differences ◽  
Antigenic Profile

A comparative study of the antigenic profile of bloodstream and cell culture derived trypomastigotes showed many differences in their components. Using mouse anti-T. cruzi antibodies the differences were located mostly in the 120 kDa band, whereas using chagasic patient sera the differences were located in the 85 and 52 kDa bands. These findings might explain known physiological differences between trypomatigotes obtained from cell culture and from infected blood. A brief report of this work has already been published9.

Presence of Trypanosoma cruzi antigen on the surface of both infected and uninfected cells in tissue culture

Parasitology ◽  
1980 ◽  
Vol 80 (1) ◽  
pp. 147-152 ◽  
Author(s):  
I. A. Abrahamsohn ◽  
J. K. Kloetzel
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Culture Medium ◽  
Culture Supernatant ◽  
Uninfected Cell ◽  
Cell Culture Supernatant ◽  
Supernatant Fluid ◽  
Cell Monolayers ◽  
Infected Cells

SummaryLLC-MK2 cell monolayers infected with Trypanosoma cruzi were shown by immunofluorescence to present parasite antigens on the surface of both parasitized and non-parasitized cells after completion of the first intracellular cycle and rupture of infected cells. The cell-culture supernatant fluid at this stage, as well as the supernatant fluid of parasites left overnight in culture medium were concentrated and contained antigen capable of binding to uninfected cell monolayers. The origin of this antigen, as well as its eventual role in the pathogenesis of Chagas' disease, are discussed.

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