scholarly journals In vitro Evaluation of the Phagocytosis Activity of Neutrophils and Characterization of Staphylococcus aureus Mastitis in Dairy Cows of Small Family Farms

Author(s):  
Valente Velazquez-Ordoñez ◽  
Nancy Montoya-Garcia ◽  
Ana María García-Gama ◽  
Gerardo Mancera Cuadros ◽  
Esvieta Tenorio-Borroto ◽  
...  
2001 ◽  
Vol 18 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Joaquim Ruiz ◽  
Josep M. Sierra ◽  
M.Teresa Jiménez De Anta ◽  
Jordi Vila

2007 ◽  
Vol 189 (24) ◽  
pp. 8871-8879 ◽  
Author(s):  
Zhibiao Fu ◽  
Niles P. Donegan ◽  
Guido Memmi ◽  
Ambrose L. Cheung

ABSTRACT The mazEF homologs of Staphylococcus aureus, designated mazEFsa , have been shown to cotranscribe with the sigB operon under stress conditions. In this study, we showed that MazEF Sa , as with their Escherichia coli counterparts, compose a toxin-antitoxin module wherein MazF Sa leads to rapid cell growth arrest and loss in viable CFU upon overexpression. MazF Sa is a novel sequence-specific endoribonuclease which cleaves mRNA to inhibit protein synthesis. Using ctpA mRNA as the model substrate both in vitro and in vivo, we demonstrated that MazF Sa cleaves single-strand RNA preferentially at the 5′ side of the first U or 3′ side of the second U residue within the consensus sequences VUUV′ (where V and V′ are A, C, or G and may or may not be identical). Binding studies confirmed that the antitoxin MazE Sa binds MazF Sa to form a complex to inhibit the endoribonuclease activity of MazF Sa . Contrary to the system in E. coli, exposure to selected antibiotics augmented mazEFsa transcription, akin to what one would anticipate from the environmental stress response of the sigB system. These data indicate that the mazEF system of S. aureus differs from the gram-negative counterparts with respect to mRNA cleavage specificity and antibiotic stresses.


Author(s):  
Muhammad Barkaat Hussain ◽  
Yasser Mahmoud Kamel ◽  
Zia Ullah ◽  
Asif Ahmad Mohamad Jiman-Fatani ◽  
Ansar Shafiq Ahmad

2020 ◽  
Vol 8 (2) ◽  
pp. 1272-1283 ◽  
Author(s):  
Vignesh Samayanpaulraj ◽  
Muthukumar Sivaramapillai ◽  
Sankara Naynar Palani ◽  
Krishnaveni Govindaraj ◽  
Vijay Velu ◽  
...  

2013 ◽  
Vol 58 (3) ◽  
pp. 239-247 ◽  
Author(s):  
Camila Nassur ◽  
Adílis Kalina Alexandria ◽  
Luciana Pomarico ◽  
Valeria Pereira de Sousa ◽  
Lúcio Mendes Cabral ◽  
...  

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