scholarly journals Alternative Strategies for Stem Cell Osteogenic Differentiation

2019 ◽  
Author(s):  
Carla Cristina Gomes Pinheiro ◽  
Daniela Franco Bueno
Keyword(s):  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Alternative Strategies

scholarly journals Platelet lysates-based hydrogels incorporating bioactive mesoporous silica nanoparticles for stem cell osteogenic differentiation

2021 ◽  
Vol 9 ◽  
pp. 100096
Author(s):  
M.T. Tavares ◽  
S.C. Santos ◽  
C.A. Custódio ◽  
J.P.S. Farinha ◽  
C. Baleizão ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesoporous Silica ◽  
Osteogenic Differentiation ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles

scholarly journals Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xudong Wang ◽  
Taiqiu Chen ◽  
Zhihuai Deng ◽  
Wenjie Gao ◽  
Tongzhou Liang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Luciferase Reporter ◽  
Specific Gene ◽  
Biological Processes ◽  
Alizarin Red ◽  
Qrt Pcr ◽  
Dual Luciferase Reporter Assay

Abstract Background Little is known about the implications of circRNAs in the effects of melatonin (MEL) on bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoporosis (OP) progression. The aim of our study was to investigate circRNAs in MEL-regulated BMSC differentiation and OP progression. Methods BMSC osteogenic differentiation was measured by qRT-PCR, western blot (WB), Alizarin Red, and alkaline phosphatase (ALP) staining. Differential circRNA and mRNA profiles of BMSCs treated by MEL were characterized by deep sequencing, followed by validation using RT-PCR, Sanger sequencing, and qRT-PCR. Silencing and overexpression of circ_0003865 were conducted for functional investigations. The sponged microRNAs and targeted mRNAs were predicted by bioinformatics and validated by qRT-PCR, RNA pull-down, and dual-luciferase reporter assay. The function of miR-3653-3p and circ_0003865/miR-3653-3p/growth arrest-specific gene 1 (GAS1) cascade was validated for the osteogenic differentiation of BMSCs by CCK-8, qRT-PCR, WB, Alizarin Red, and ALP staining. The effects of circ_0003865 on OP development were tested in murine OP model. Results MEL promoted osteogenic differentiation of BMSCs. RNA sequencing revealed significant alterations in circRNA and mRNA profiles associated with multiple biological processes and signaling pathways. Circ_0003865 expression in BMSCs was significantly decreased by MEL treatment. Silencing of circ_0003865 had no effect on proliferation while promoted osteogenic differentiation of BMSCs. Overexpression of circ_0003865 abrogated the promotion of BMSC osteogenic differentiation induced by MEL, but proliferation of BMSCs induced by MEL had no change whether circ_0003865 was overexpression or not. Furthermore, circ_0003865 sponged miR-3653-3p to promote GAS1 expression in BMSCs. BMSC osteogenic differentiation was enhanced by miR-3653-3p overexpression while BMSC proliferation was not affected. By contrast, miR-3653-3p silencing mitigated the promoted BMSC osteogenic differentiation caused by circ_0003865 silencing, but had no effect on proliferation. Finally, circ_0003865 silencing repressed OP development in mouse model. Conclusion MEL promotes BMSC osteogenic differentiation and inhibits OP pathogenesis by suppressing the expression of circ_0003865, which regulates GAS1 gene expression via sponging miR-3653-3p.

scholarly journals Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0193059 ◽  
Author(s):  
Xiaoqin Wang ◽  
Omar Omar ◽  
Forugh Vazirisani ◽  
Peter Thomsen ◽  
Karin Ekström
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation

Functions of the Mg–HA coating on carbon/carbon composite surface to promote the proliferation and osteogenic differentiation of mBMSCs

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105056-105062 ◽  
Author(s):  
Xiong Xinbo ◽  
Ni Xinye ◽  
Zhou Dong
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Carbon Composite ◽  
Composite Surface ◽  
Ha Coating

This study aims to evaluate the functions of the Mg–hydroxyapatite (Mg–HA) bio-coating on carbon/carbon composite (C/C) surface to promote the proliferation and osteogenic differentiation of bone mesenchymal stem cell (BMSCs).

Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation

2021 ◽  
Vol 13 (11) ◽  
pp. 1699-1716
Author(s):  
Jelena Krstić ◽  
Slavko Mojsilović ◽  
Sonja S Mojsilović ◽  
Juan F Santibanez
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Fate ◽  
Interleukin 17 ◽  
Stem Cell Fate

Osteon-mimetic 3D nanofibrous scaffold enhancing stem cell proliferation and osteogenic differentiation for bone regeneration

2022 ◽  
Author(s):  
Ting Song ◽  
Jianhua Zhou ◽  
Ming Shi ◽  
Liuyang Xuan ◽  
Huamin Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Nanofibrous Scaffold ◽  
Hierarchical Microstructure ◽  
Stem Cell Proliferation

Scaffold microstructure is important for bone tissue engineering. Failure to synergistically imitate the hierarchical microstructure of bone component, such as osteon with concentric multilayers assembled by nanofibers, hindered the performance...

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