scholarly journals New Tools for Dengue Diagnostics

2019 ◽  
Author(s):  
Om Parkash ◽  
Punam Kumari ◽  
Vasu Deva ◽  
Sham Lal ◽  
Javed Ahmed Ujjan ◽  
...  
Keyword(s):  
Dengue Diagnostics

scholarly journals Non-Invasive Dengue Diagnostics—The Use of Saliva and Urine for Different Stages of the Illness

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1345
Author(s):  
Mahathir Humaidi ◽  
Wei Ping Tien ◽  
Grace Yap ◽  
Choon Rong Chua ◽  
Lee Ching Ng
Keyword(s):  
Clinical Symptoms ◽  
Detection Algorithm ◽  
Acute Stage ◽  
Serum Samples ◽  
Non Invasive ◽  
Fever Onset ◽  
Dengue Transmission ◽  
Dengue Diagnostics ◽  
Potential Tool

Dengue diagnosis is largely dependent on clinical symptoms and routinely confirmed with laboratory detection of dengue virus in patient serum samples collected via phlebotomy. This presents a challenge to patients not amenable to venipuncture. Non-invasive methods of dengue diagnosis have the potential to enhance the current dengue detection algorithm. In this study, samples from dengue infected patients were collected between January 2012 until September 2012 and September 2013 until December 2013 in two different setups. Panel A samples (blood, urine, and saliva) were collected daily when the 39 patients were hospitalised and during their follow-up visits while Panel B samples (saliva) were collected from 23 patients during the acute stage of dengue. Using DENV PCR on Panel A, from day 2 to day 4 post fever onset, serum showed the best overall positivity followed by saliva and urine (100%/82.1%/67.9%). From day 5 until day 10 post fever onset, serum and urine had similar positivity (67.4%/61.2%), followed by saliva (51.3%). Beyond day 10 post fever onset, DENV was undetectable in sera, but urine and saliva showed 56.8% and 28.6% positivity, respectively. DENV in urine was detectable up until 32 days post fever. Panel B results showed overall sensitivity of 32.4%/36% (RNA/NS1) for DENV detection in saliva. Our results suggest that the urine-based detection method is useful especially for late dengue detection, where DENV is undetected in sera but still detectable in urine. This provides a potential tool for the physician to pick up new cases in an area where there is ongoing dengue transmission and subsequently prompt for intensified vector control activities.

Approach to non-invasive sampling in dengue diagnostics: Exploring virus and NS1 antigen detection in saliva and urine of travelers with dengue

2014 ◽  
Vol 61 (3) ◽  
pp. 353-358 ◽  
Author(s):  
Essi M. Korhonen ◽  
Eili Huhtamo ◽  
Anna-Maija K. Virtala ◽  
Anu Kantele ◽  
Olli Vapalahti
Keyword(s):  
Antigen Detection ◽  
Non Invasive ◽  
Ns1 Antigen ◽  
Dengue Diagnostics

scholarly journals Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein

2013 ◽  
Vol 16 (4) ◽  
pp. 609 ◽  
Author(s):  
Advaita Ganguly ◽  
Ravindra B. Malabadi ◽  
Dipankar Das ◽  
Mavanur R. Suresh ◽  
Hoon H Sunwoo
Keyword(s):  
Dengue Virus ◽  
Envelope Protein ◽  
Vaccine Development ◽  
Virus Envelope ◽  
E Coli ◽  
C Terminus ◽  
Virus Envelope Protein ◽  
Biological Functionality ◽  
Dengue Diagnostics

Purpose. To highlight the expression and purification of the recombinant dengue virus type-1 antigen exploiting the codon optimized full length envelope for increased yield in E. coli. Methods. A 6x His tag was inserted at the C terminus to facilitate purification. The purified protein was recognized in Western blot by Monoclonal antibody specific for the tag. The in vitro refolded recombinant protein was used to immunize mice for the development of hybridomas and also analyzed for its biological functionality with heparan sulfate binding assay. Results. The polyclonal anti-sera from the immunized mice were found to recognize the envelope protein thereby establishing the immunogenicity of the protein. Conclusion. The purified envelope protein could potentially be used towards dengue diagnostics and vaccine development efforts. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals First round of external quality assessment of dengue diagnostics in the WHO Western Pacific Region, 2013

2015 ◽  
Vol 6 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Kwoon Yong Pok ◽  
Raynal C Squires ◽  
Li Kiang Tan ◽  
Tomohiko Takasaki ◽  
Sazaly Abubakar ◽  
...  
Keyword(s):  
Quality Assessment ◽  
External Quality Assessment ◽  
Western Pacific ◽  
Pacific Region ◽  
Western Pacific Region ◽  
External Quality ◽  
Dengue Diagnostics

scholarly journals Current Global Status of Dengue Diagnostics

2015 ◽  
Vol 2 (2) ◽  
pp. 79-95 ◽  
Author(s):  
Miranda Smith ◽  
Azliyati Azizan
Keyword(s):  
Global Status ◽  
Dengue Diagnostics

scholarly journals Correction to: Improving Dengue Diagnostics and Management Through Innovative Technology

2018 ◽  
Vol 20 (8) ◽  
Author(s):  
Jesus Rodriguez-Manzano ◽  
Po Ying Chia ◽  
Tsin Wen Yeo ◽  
Alison Holmes ◽  
Pantelis Georgiou ◽  
...  
Keyword(s):  
Innovative Technology ◽  
Dengue Diagnostics

scholarly journals Alanine Substitution Inactivates Cross-Reacting Epitopes in Dengue Virus Recombinant Envelope Proteins

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 208
Author(s):  
Viviana C. Zomosa-Signoret ◽  
Karina R. Morales-González ◽  
Ana E. Estrada-Rodríguez ◽  
Ana M. Rivas-Estilla ◽  
M. Cristina Devèze-García ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Rural Areas ◽  
Cross Reactivity ◽  
Diagnostic Methods ◽  
Hemorrhagic Complication ◽  
Virus Envelope ◽  
Recombinant Peptide ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Diagnostics

The expansion of the habitat of mosquitoes belonging to the Aedes genus puts nearly half of the world’s population at risk of contracting dengue fever, and a significant fraction will develop its serious hemorrhagic complication, which can be fatal if not diagnosed properly and treated in a timely fashion. Although several diagnostic methods have been approved for dengue diagnostics, their applicability is limited in rural areas of developing countries by sample preparation costs and methodological requirements, as well as cross-reactivity among the different serotypes of the Dengue virus and other flavivirus, such as the Zika virus. For these reasons, it is necessary to generate more specific antigens to improve serological methods that could be cheaper and used in field operations. Here, we describe a strategy for the inactivation of cross-reacting epitopes on the surface of the Dengue virus envelope protein through the synthetic generation of recombinant peptide sequences, where key amino acid residues from Dengue virus serotype 1 (DENV-1) and 2 (DENV-2) are substituted by alanine residues. The proteins thus generated are recognized by 88% of sera from Dengue NS1+ patients and show improved serotype specificity because they do not react with the antibodies present in seroconverted, PCR-serotyped DEN-4 infected patients.

scholarly journals Dengue diagnostics: serious inaccuracies are likely to occur if pre-analytical conditions are not strictly followed

2020 ◽  
Vol 115 ◽  
Author(s):  
Felipe Campos de Melo Iani ◽  
Ana Carolina Barbosa Caetano ◽  
Jéssica Caroline Wenceslau Cocovich ◽  
Frederico Figueiredo Amâncio ◽  
Maira Alves Pereira ◽  
...  
Keyword(s):  
Dengue Diagnostics

scholarly journals Rapid and accurate interpretation of dengue diagnostics in the context of dengue vaccination implementation: Viewpoints and guidelines issued from an experts group consultation

2017 ◽  
Vol 11 (9) ◽  
pp. e0005719 ◽  
Author(s):  
Elizabeth A. Hunsperger ◽  
Claudia N. Duarte dos Santos ◽  
Huong Thi Que Vu ◽  
Sutee Yoksan ◽  
Vincent Deubel
Keyword(s):  
Accurate Interpretation ◽  
Dengue Diagnostics ◽  
Group Consultation

scholarly journals A review of dengue diagnostics and implications for surveillance and control

2019 ◽  
Vol 113 (11) ◽  
pp. 653-660 ◽  
Author(s):  
Nader Raafat ◽  
Stuart D Blacksell ◽  
Richard J Maude
Keyword(s):  
Disease Surveillance ◽  
Dengue Infection ◽  
Signs And Symptoms ◽  
Diagnostic Methods ◽  
Control Measures ◽  
Polymerase Chain ◽  
Dengue Diagnostics ◽  
Dengue Control ◽  
Age Range ◽  
And Control

Abstract Dengue is the world’s most common arboviral infection, with almost 4 billion people estimated to be living at risk of dengue infection. A recently introduced vaccine is currently recommended only for seropositive individuals in a restricted age range determined by transmission intensity. With no effective dengue vaccine for the general population or any antiviral therapy, dengue control continues to rely heavily on vector control measures. Early and accurate diagnosis is important for guiding appropriate management and for disease surveillance to guide prompt dengue control interventions. However, major uncertainties exist in dengue diagnosis and this has important implications for all three. Dengue can be diagnosed clinically against predefined lists of signs and symptoms and by detection of dengue-specific antibodies, non-structural 1 antigen or viral RNA by reverse transcriptase–polymerase chain reaction. All of these methods have their limitations. This review aims to describe and quantify the advantages, uncertainties and variability of the various diagnostic methods used for dengue and discuss their implications and applications for dengue surveillance and control.

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