scholarly journals Authenticating Hybrid Cell Lines

Cell Culture ◽  
2019 ◽  
Author(s):  
Raymond W. Nims ◽  
Amanda Capes-Davis ◽  
Christopher Korch ◽  
Yvonne A. Reid
Keyword(s):  
Cell Lines ◽  
Hybrid Cell ◽  
Hybrid Cell Lines

Extinction and activation of the thyroglobulin promoter in hybrids of differentiated and transformed thyroid cells

1990 ◽  
Vol 10 (3) ◽  
pp. 1033-1040
Author(s):  
I M Bonapace ◽  
M Sanchez ◽  
S Obici ◽  
A Gallo ◽  
S Garofalo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Selectable Marker ◽  
Hybrid Cell ◽  
Transformed Cells ◽  
Thyroid Cells ◽  
Dominant Selectable Marker ◽  
Thyroglobulin Gene ◽  
Rat Thyroid ◽  
Hybrid Cell Lines

Thyroglobulin gene expression was repressed in a rat thyroid cell line transformed with Kirsten murine sarcoma virus. Expression of a dominant selectable marker driven by the thyroglobulin promoter was also inhibited. Somatic cell hybridization of transformed and differentiated thyroid cells resulted in extinction of thyroglobulin gene expression. When transformed cells carrying a dominant selectable marker driven by the thyroglobulin promoter were fused to differentiated cells and expression of this marker was selected, we obtained stable hybrid cell lines expressing both the endogenous and the exogenous thyroglobulin promoters. Although the expression of v-ras remained unchanged compared with expression in the parental transformed cells, transformation was suppressed in the hybrid cell lines. The other thyroid differentiation markers, iodide uptake and thyroid-stimulating hormone-dependent growth, were inhibited in all the hybrids tested. We show that activity of the thyroglobulin promoter correlates with the presence of a thyroid nuclear factor that binds the promoter at position -60 from the transcription start site. Loss of this factor accompanies the extinction of thyroglobulin gene expression in hybrids selected for expression of a non-thyroid-specific promoter.

Embryonic stem cells can be used to construct hybrid cell lines containing a single, selectable murine chromosome

1999 ◽  
Vol 10 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Petra M. Jakobs ◽  
Lesley Smith ◽  
Mathew Thayer ◽  
Markus Grompe
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Embryonic Stem ◽  
Murine Chromosome ◽  
Hybrid Cell Lines

scholarly journals Propagation of Hepatitis A Virus in Hybrid Cell Lines Derived from Marmoset Liver and Vero Cells

1989 ◽  
Vol 70 (9) ◽  
pp. 2487-2494 ◽  
Author(s):  
M. Ashida ◽  
H. Hara ◽  
H. Kojima ◽  
T. Kamimura ◽  
F. Ichida ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Hybrid Cell ◽  
Vero Cells ◽  
Hybrid Cell Lines

Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome

Genomics ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Amelia A. Langston ◽  
Cathryn S. Mellersh ◽  
Cassandra L. Neal ◽  
Kunal Ray ◽  
Gregory M. Acland ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hybrid Cell ◽  
Canine Genome ◽  
Hybrid Cell Lines

Accessory function of human leukemic cell lines: properties of B and B-K562 hybrid cell lines

1985 ◽  
Vol 15 (3) ◽  
pp. 256-261 ◽  
Author(s):  
Hiro Wakasugi ◽  
Marie C. Dokhelar ◽  
Dominique Garson ◽  
Annick Harel-Bellan ◽  
Didier Fradelizi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hybrid Cell ◽  
Leukemic Cell ◽  
Human Leukemic Cell ◽  
Leukemic Cell Lines ◽  
Accessory Function ◽  
Hybrid Cell Lines
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