scholarly journals Laboratory Tests Used in the Diagnostic and Research of Dengue Virus: Present and Future

Author(s):  
Juan Samuel Sulca Herencia
2013 ◽  
Vol 4 (4) ◽  
pp. 46
Author(s):  
Rahayu Anggraini ◽  
Nasronudin Nasronudin

Background: In Indonesia has four serotypes, the DEN-1, DEN-2, DEN-3 and DEN-4. The management of Dengue virus becomes difficult because the patients were infected with different clinical profiles depending on the serotypes and genotypes of infecting dengue virus. Consequently, the diagnosis and treatment becomes difficult. Aim: The purpose of this study was to identify the difference between the results of laboratory tests between non-dengue fever and positive dengue fever. Method: This study was an observational cross-sectional study. Fifteen samples were diagnosed with dengue fever and fifteen samples with negative dengue fever on NS1, IgM / IgG-anti-DHF strip test results. Laboratory tests comprising whole blood, SGOT, SGPT, and TNFa were first examined when the patient came to the hospital. The collected data were analyzed by Chi-Square test SPSS version 13 for Windows. Result: The results of the study in two groups regarding sex, age, days of fever, grade, hemoglobin levels, leukocytes count, platelet count, hematocrit percentage, SGOT levels, and TNFa level were not significantly different with p > 0.050, whereas the SGPT level in non-dengue increased 3 x of normal value of 66.7%, n = 10/15 and in positive dengue fever the SGPT level was within normal limits, found in 60%, n = 9/15, so there was significant difference with p = 0.022 (p < 0.05). Conclusion: in non-dengue SGOT and SGPT levels increased of 1–3 times the normal value. In positive dengue fever SGOT levels increased 1–3 x normal value, but SGPT levels was within normal value, so SGPT levels can be used as a predictive factor for distinguishing the two types of fever.


2015 ◽  
Vol 58 (3) ◽  
pp. 328 ◽  
Author(s):  
Shashikala Nair ◽  
Anandhalakshmi Subramaniyan ◽  
Philip VivianJoseph Ratnam ◽  
Reba Kanungo ◽  
Harshavardhini Palanivel

2010 ◽  
Vol 2 (2) ◽  
pp. 95-98
Author(s):  
Lukman Hakim ◽  
Asep Jajang Kusnandar

Dengue hemorrhagic fever (DHF) is an infectious disease caused by dengue virus with the widest spread in the Asian region. In Indonesia, every year is always an outbreak in some provinces, the largest occurred in 1998 and 2004 with the number of patient mortality by 79 480 people with 800 people. To know the risk factor for dengue transmission in Cirebon District that had a high DHF cases, had been conducted the research with calculating the number and density of humans at the houses and also survey of density of Aedes aegypti pre-adult and adult stages.The research was resulting that the house index (HI) of Ae. aegypti is 58%, while homes found Ae. aegypti adults is 46%, 6 of which house is the house that is not found larvae / pupae of mosquitoes. The laboratory tests found that the frequency of biting Ae. aegypti mosquitoes average 4.5 times per day, at least 2 times and no more than 7 times per day. Number of people bitten by adult mosquitoes average of 3.1 people per day, is at least 2 people and maximum 5 people per day. Based on the statistically test, it is known that there is significant correlation between the number of inhabitants of the house and the presence of mosquito larvae / pupa of Ae. aegypti mosquito with biting frequency, whereas the most dominant variable was the presence of larvae / pupae of mosquitoes. Furthermore, it is advisable to do the cleaning intensification of Aedes breeding places inand outside the home including those hidden. It is also necessary to place residential settings so that the number of occupants per house so less.


VASA ◽  
2001 ◽  
Vol 30 (Supplement 58) ◽  
pp. 21-27
Author(s):  
Luther

In diabetic foot disease, critical limb ischaemia (CLI) cannot be precisely described using established definitions. For clinical use, the Fontaine classification complemented with any objective verification of a reduced arterial circulation is sufficient for decision making. For scientific purposes, objective measurement criteria should be reported. Assessment of CLI should rely on the physical examination of the limb arteries, complemented by laboratory tests like the shape of the PVR curve at ankle or toe levels, and arteriography. The prognosis of CLI in diabetic foot disease depends on the success of arterial reconstruction. The best prognosis for the patients is with a preserved limb. Reconstructive surgery is the best choice for the majority of patients.


2012 ◽  
Vol 17 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Rosalind Potts ◽  
Robin Law ◽  
John F. Golding ◽  
David Groome

Retrieval-induced forgetting (RIF) refers to the finding that the retrieval of an item from memory impairs the retrieval of related items. The extent to which this impairment is found in laboratory tests varies between individuals, and recent studies have reported an association between individual differences in the strength of the RIF effect and other cognitive and clinical factors. The present study investigated the reliability of these individual differences in the RIF effect. A RIF task was administered to the same individuals on two occasions (sessions T1 and T2), one week apart. For Experiments 1 and 2 the final retrieval test at each session made use of a category-cue procedure, whereas Experiment 3 employed category-plus-letter cues, and Experiment 4 used a recognition test. In Experiment 2 the same test items that were studied, practiced, and tested at T1 were also studied, practiced, and tested at T2, but for the remaining three experiments two different item sets were used at T1 and T2. A significant RIF effect was found in all four experiments. A significant correlation was found between RIF scores at T1 and T2 in Experiment 2, but for the other three experiments the correlations between RIF scores at T1 and T2 failed to reach significance. This study therefore failed to find clear evidence for reliable individual differences in RIF performance, except where the same test materials were used for both test sessions. These findings have important implications for studies involving individual differences in RIF performance.


1974 ◽  
Vol 32 (02/03) ◽  
pp. 483-491
Author(s):  
E. A Loeliger ◽  
M. J Boekhout-Mussert ◽  
L. P van Halem-Visser ◽  
J. D. E Habbema ◽  
H de Jonge

SummaryThe present study concerned the reproducibility of the so-called prothrombin time as assessed with a series of more commonly used modifications of the Quick’s onestage assay procedure, i.e. the British comparative reagent, homemade human brain thromboplastin, Simplastin, Simplastin A, and Thrombotest. All five procedures were tested manually on pooled lyophilized normal and patients’ plasmas. In addition, Simplastin A and Thrombotest were investigated semiautomatically on individual freshly prepared patients’ plasmas. From the results obtained, the following conclusions may be drawn :The reproducibility of results obtained with manual reading on lyophilized plasmas is satisfactory for all five test procedures. For Simplastin, the reproducibility of values in the range of insufficient anticoagulation is relatively low due to the low discrimination power of the test procedure in the near-normal range (so-called low sensitivity of rabbit brain thromboplastins). The reproducibility of Thrombotest excels as a consequence of its particularly easily discerned coagulation endpoint.The reproducibility of Thrombotest, when tested on freshly prepared plasmas using Schnitger’s semiautomatic coagulometer (a fibrinometer-liJce apparatus), is no longer superior to that of Simplastin A.The constant of proportionality between the coagulation times formed with Simplastin A and Thrombotest was estimated at 0.64.Reconstituted Thrombotest is stable for 24 hours when stored at 4° C, whereas reconstituted Simplastin A is not.The Simplastin A method and Thrombotest seem to be equally sensitive to “activation” of blood coagulation upon storage.


1964 ◽  
Vol 11 (02) ◽  
pp. 506-512 ◽  
Author(s):  
V. A Lovric ◽  
J Margolis

SummaryAn adaptation of “kaolin clotting time” and prothrombin time for use on haemolysed capillary blood provided simple and sensitive screening tests suitable for use in infants and children. A survey of three year’s experience shows that these are reliable routine laboratory tests for detection of latent coagulation disorders.


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