scholarly journals Clinical Pharmacokinetics of Clavulanic Acid, a Novel β- Lactamase Isolated from Streptomyces clavuligerus and Its Variability

2019 ◽  
Author(s):  
Anab Fatima ◽  
Mohammad Jiyad Shaikh ◽  
Hina Zahid ◽  
Ishart Younus ◽  
Sheikh Abdul Khaliq ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Streptomyces Clavuligerus ◽  
Clinical Pharmacokinetics

scholarly journals Enhanced production of Clavulanic acid by improving glycerol utilization using reporter-guided mutagenesis of an industrial Streptomyces clavuligerus strain

2021 ◽  
Author(s):  
Chang-Hun Shin ◽  
Hang Soo Cho ◽  
Hyung-Jin Won ◽  
Ho Jeong Kwon ◽  
Chan-Wha Kim ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Wild Type Strain ◽  
Random Mutagenesis ◽  
Streptomyces Clavuligerus ◽  
Wild Type ◽  
Mutant Selection ◽  
Enhanced Production ◽  
Industrial Strains ◽  
Glycerol Utilization

Abstract Clavulanic acid (CA) produced by Streptomyces clavuligerus is a clinically important β-lactamase inhibitor. It is known that glycerol utilization can significantly improve cell growth and CA production of S. clavuligerus. We found that the industrial CA-producing S. clavuligerus strain OR generated by random mutagenesis consumes less glycerol than the wild-type strain; we then developed a mutant strain in which the glycerol utilization operon is overexpressed, as compared to the parent OR strain, through iterative random mutagenesis and reporter-guided selection. The CA production of the resulting S. clavuligerus ORUN strain was increased by approximately 31.3 per cent (5.21 ± 0.26 g/L) in a flask culture and 17.4 per cent (6.11 ± 0.36 g/L) in a fermenter culture, as compared to that of the starting OR strain. These results confirmed the important role of glycerol utilization in CA production and demonstrated that reporter-guided mutant selection is an efficient method for further improvement of randomly mutagenized industrial strains.

scholarly journals Bibliometric Analysis of Global Research on Clavulanic Acid

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 102 ◽  
Author(s):  
Howard Ramirez-Malule
Keyword(s):  
Clavulanic Acid ◽  
Bibliometric Analysis ◽  
Chemical Engineering ◽  
Streptomyces Clavuligerus ◽  
The United States ◽  
Subject Area ◽  
Scientific Production ◽  
Resistant Bacteria ◽  
The United Kingdom ◽  
The Impact

Clavulanic acid (CA), a potent inhibitor of the β-lactam, ase enzyme, is frequently co-formulated with a broad spectrum of antibiotics to treat infections caused by β-lactamase-producing pathogens. In order to evaluate the impact and the progress of CA studies in the last four decades, a bibliometric analysis of the global scientific production of CA was carried out. A total of 39,758 records in the field of CA were indexed in the Scopus database for a 43-year period of study (1975–2017). The results indicated that CA studies have grown, showing three phases (1975–1999, 2000–2003 and 2004–2017) based on records of publications; the results showed a sigmoidal profile. Medicine was the main subject area for CA studies, whereas biochemistry, genetics and molecular biology were areas of research for CA production by Streptomyces clavuligerus (S. clavuligerus). Nevertheless, chemical engineering (as a subject area) had the highest increase in the percentage of publications related to CA production by S. clavuligerus. The United States, France, the United Kingdom, Spain and Brazil were the leading countries in the scientific production of studies on both CA and CA related to S. clavuligerus. This analysis allowed the identification of the area of knowledge with the highest impact on CA studies, the top researchers and their geographic distribution, and also helped to highlight the existence of antibiotic-resistant bacteria as an emergent area in CA research.

scholarly journals In vivo functional analysis of a class A β-lactamase-related protein essential for clavulanic acid biosynthesis in Streptomyces clavuligerus

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0215960 ◽  
Author(s):  
Santosh K. Srivastava ◽  
Kelcey S. King ◽  
Nader F. AbuSara ◽  
Chelsea J. Malayny ◽  
Brandon M. Piercey ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Clavulanic Acid ◽  
Streptomyces Clavuligerus ◽  
Related Protein ◽  
Acid Biosynthesis ◽  
Class A

Three unlinked gene clusters are involved in clavam metabolite biosynthesis in Streptomyces clavuligerus

2004 ◽  
Vol 50 (10) ◽  
pp. 803-810 ◽  
Author(s):  
Kapil Tahlan ◽  
Hyeon Ung Park ◽  
Susan E Jensen
Keyword(s):  
Clavulanic Acid ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Biosynthetic Pathway ◽  
Gene Clusters ◽  
Streptomyces Clavuligerus ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Family ◽  
Chromosome Walking ◽  
Restriction Fragments

In Streptomyces clavuligerus, three groups of genes are known to be involved in the biosynthesis of the clavam metabolites. Since antibiotic biosynthetic genes are invariably clustered on the chromosome in prokaryotes, chromosome walking was undertaken in an attempt to show that the three groups of clavam genes would resolve into a single super-cluster when analyzed at larger scale. However, no evidence of linkage between the three groups was obtained. Furthermore, Southern analysis of macro-restriction fragments of genomic DNA separated by pulsed-field gel electrophoresis also indicated that the three groups of genes are not linked. Despite the structural and biosynthetic relatedness of the clavam metabolites, our results suggest that the genes involved in their production lie in three unlinked gene clusters. We believe that this represents the first instance in bacteria of genes involved in the biosynthesis of a single family of antibiotics sharing a common biosynthetic pathway and yet residing in three separate locations on the chromosome.Key words: Streptomyces, clavulanic acid, clavams, paralogues, gene clusters.

Improvement for the production of clavulanic acid by mutant Streptomyces clavuligerus

2002 ◽  
Vol 34 (5) ◽  
pp. 370-375 ◽  
Author(s):  
S.D. Lee ◽  
S.W. Park ◽  
K.K. Oh ◽  
S.I. Hong ◽  
S.W. Kim
Keyword(s):  
Clavulanic Acid ◽  
Streptomyces Clavuligerus

scholarly journals Glycerol Utilization Gene Cluster in Streptomyces clavuligerus

2009 ◽  
Vol 75 (9) ◽  
pp. 2991-2995 ◽  
Author(s):  
Sonia Baños ◽  
Rosario Pérez-Redondo ◽  
Bert Koekman ◽  
Paloma Liras
Keyword(s):  
Clavulanic Acid ◽  
Gene Cluster ◽  
Acid Production ◽  
Type Strain ◽  
Wild Type Strain ◽  
Streptomyces Clavuligerus ◽  
Wild Type ◽  
Glycerol Utilization ◽  
In The Wild

ABSTRACT The Streptomyces clavuligerus ATCC 27064 glycerol cluster gylR-glpF1K1D1 is induced by glycerol but is not affected by glucose. S. clavuligerus growth and clavulanic acid production are stimulated by glycerol, but this does not occur in a glpK1-deleted mutant. Amplification of glpK1D1 results in transformants yielding larger amounts of clavulanic acid in the wild-type strain and in overproducer S. clavuligerus Gap15-7-30 or S. clavuligerus ΔrelA strains.

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