scholarly journals Statistical Optimization of Acetoin Production Using Corn Steep Liquor as a Low-Cost Nitrogen Source by Bacillus Subtilis CICC 10025

2019 ◽  
Author(s):  
Abiola Ezekiel Taiwo ◽  
Tunde Victor Ojumu ◽  
Tafirenyika N. Madzimbamuto
Keyword(s):  
Bacillus Subtilis ◽  
Nitrogen Source ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Statistical Optimization ◽  
Acetoin Production ◽  
Steep Liquor

scholarly journals Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Genan Wang ◽  
Bingyi Shi ◽  
Pan Zhang ◽  
Tingbin Zhao ◽  
Haisong Yin ◽  
...  
Keyword(s):  
Malic Acid ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Enrichment Analysis ◽  
Tca Cycle ◽  
Water Soluble ◽  
Growth And Yield ◽  
The Tca Cycle ◽  
Steep Liquor ◽  
Aureobasidium Melanogenum

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

scholarly journals Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

2020 ◽  
Vol 12 (17) ◽  
pp. 7122
Author(s):  
Ludwika Tomaszewska-Hetman ◽  
Waldemar Rymowicz ◽  
Anita Rywińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Culture Broth ◽  
Biodiesel Production ◽  
Innovative Strategy ◽  
Simple Medium ◽  
Low Cost Strategy ◽  
Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

Organic Acid Production by Propionibacterium shermanii: Effect of pH, Temperature and Vitamin-Nitrogen Source

1997 ◽  
Vol 52 (3-4) ◽  
pp. 193-196 ◽  
Author(s):  
A. Quesada-Chanto ◽  
A . G. Schroeder ◽  
A. C. Schmid-Meyer ◽  
J. A. López ◽  
M. M. Silveira ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Propionic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Ph Value ◽  
Corn Steep Liquor ◽  
Organic Acid Production ◽  
Propionibacterium Shermanii ◽  
Difco Yeast Extract ◽  
Steep Liquor

Abstract Production of propionic acid by Propionibacterium shermanii CDB 10014 was enhanced by a pH value of 6.5 and by temperatures in the range 35-37 °C. Depending on the type of yeast extract, succinic acid can be produced in higher proportions, with decreasing propionic acid yields. With respect to propionic acid production, Difco yeast extract has shown the best results when yeast extract preparations from other different suppliers were compared. To replace yeast extract by a cheaper vitamin-nitrogen source, corn-steep liquor was tested. A complete depletion of glucose was achieved, yielding a final propionic acid concentration of over 35 g/l. These results are even better than those obtained with Difco yeast extract and suggest the possibility of an economical process based on corn-steep liquor.

scholarly journals Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020 ◽  
Vol 10 (3) ◽  
pp. 5348-5354
Keyword(s):  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Aeration Rate ◽  
Phaffia Rhodozyma ◽  
Agitation Rate ◽  
Fed Batch ◽  
Batch Bioreactor ◽  
Steep Liquor ◽  
Β Carotene

This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

scholarly journals PENINGKATAN PRODUKSI SEFALOSPORIN C DARI Acremonium chrysogenum CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN

2017 ◽  
Vol 4 (1) ◽  
pp. 10
Author(s):  
Erwahyuni Prabandari ◽  
Dyah Noor Hidayati ◽  
Diana Dewi ◽  
Eni Dwi Islamiati ◽  
Khaswar Syamsu
Keyword(s):  
Nitrogen Source ◽  
Corn Steep Liquor ◽  
Medium Composition ◽  
Production Optimization ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Carbon And Nitrogen ◽  
Lactam Antibiotic ◽  
Cephalosporin C Production ◽  
Steep Liquor

Cephalosporin is a β-lactam antibiotic produced by Acremonium chrysogenum using submerged fermentation. Carbon and nitrogen are the most influential medium ingredients for cephalosporin formation. The purpose of this study was to obtain the best composition of media for cephalosporin C production. Response surface methodology was used for production optimization. The results showed that molasses of 70 g/Lwas the best carbon source, while the best nitrogen source was the combination of corn steep liquor, urea and ammonium sulphate. DL-methionine, carbon, and nitrogen source significantly affected  the production of cephalosporin C. The mathematically modelled optimization showed that the highest production of cephalosporin C (3876 mg/L) was obtained using medium composition of 68.28 g/L molasses, 71.61 g/L nitrogen, and 0.4 g/L DL-methionine. Laboratory verification using the same medium composition produced 3696 mg/L of cephalosporin C, being 4.65% different from the mathematically optimized results. Medium optimization increased the cephalosprin C production which was 1.48 times higher than that using the previous medium, where the maximum production was only 2487 mg / L.Keywords: Carbon,  cephalosporin C, cultivation medium, nitrogen, A. chrysogenum ABSTRAKSefalosporin C adalah golongan antibiotik β-lactam yang dihasilkan Acremonium chrysogenum melalui fermentasi cair. Komponen yang sangat berpengaruh terhadap produksi sefalosporin C adalah sumber karbon dan nitrogen. Penelitian ini bertujuan mendapatkan komposisi media terbaik untuk produksi sefalosporin C. Optimasi dilakukan menggunakan metode respon permukaan. Hasil menunjukkan bahwa molases 70 g/L adalah sumber karbon terbaik dan kombinasi corn steep liquor, urea dan ammonium sulfat adalah sumber nitrogen terbaik. DL-methionin, sumber karbon, dan nitrogen berpengaruh nyata terhadap produksi sefalosporin C. Optimasi menggunakan model matematika menunjukkan produksi sefalosporin C tertinggi (3876 mg/L) yang diperoleh dengan komposisi media 68,28 g/L molases, 71,61 g/L nitrogen, dan  0,4 g/L DL-methionin. Verfikasi di laboratorium menggunakan komposisi media yang sama menghasilkan sefalosporin C sebesar 3696 mg/L, berbeda 4,65% dibanding dengan hasil optimasi matematis. Optimasi media mampu meningkatkan produksi sefalosprin C sebesar 1,48 kali dibanding media yang digunakan sebelumnya, dimana maksimal hanya menghasilkan 2487 mg/L.Kata kunci: Karbon, sefalosporin C, media kultivasi, nitrogen, A. chrysogenum

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