scholarly journals Marker-Assisted Selection (MAS): A Fast-Track Tool in Tomato Breeding

2019 ◽  
Author(s):  
Michael K. Osei ◽  
Ruth Prempeh ◽  
Joseph Adjebeng-Danquah ◽  
Jacinta A. Opoku ◽  
Agyemang Danquah ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Fast Track ◽  
Tomato Breeding

Marker-assisted selection for fast-track breeding of high oleic lines in safflower (Carthamus tinctorious L.)

2020 ◽  
Vol 158 ◽  
pp. 112983
Author(s):  
Palchamy Kadirvel ◽  
Cheelam Veerraju ◽  
Senapathy Senthilvel ◽  
Praduman Yadav ◽  
Betha Usha Kiran ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Fast Track ◽  
High Oleic ◽  
Selection For

scholarly journals Development of a New Molecular Marker for the Resistance to Tomato Yellow Leaf Curl Virus

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Adedze Yawo Mawunyo Nevame ◽  
Lu Xia ◽  
Chofong Gilbert Nchongboh ◽  
Muhammad Mahmudul Hasan ◽  
Md. Amirul Alam ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Gene Sequence ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Breeding Lines ◽  
Tomato Breeding ◽  
Green Port ◽  
Susceptible Tomato ◽  
Leaf Curl Virus ◽  
Leaf Curl

Tomato yellow leaf curl virus(TYLCV) responsible for tomato yellow leaf curl disease (TYLCD) causes a substantial decrease in tomato (Solanum lycopersicumL.) yield worldwide. The use of resistant variety as a sustainable management strategy has been advocated. Tremendous progress has been made in genetically characterizing the resistance genes (Rgene) in tomato. Breeding tomato for TYLCV resistance has been based mostly onTy-3as a race-specific resistance gene by introgression originating from wild tomato species relatives. Improvement or development of a cultivar is achievable through the use of marker-assisted selection (MAS). Therefore, precise and easy use of gene-targeted markers would be of significant importance for selection in breeding programs. The present study was undertaken to develop a new marker based onTy-3gene sequence that can be used for MAS in TYLCV resistant tomato breeding program. The new developed marker was named ACY. The reliability and accuracy of ACY were evaluated against those ofTy-3linked marker P6-25 through screening of commercial resistant and susceptible tomato hybrids, and genetic segregation using F2 population derived from a commercial resistant hybrid AG208. With the use of bioinformatics and DNA sequencing analysis tools, deletion of 10 nucleotides was observed inTy-3gene sequence for susceptible tomato variety. ACY is a co-dominant indel-based marker that produced clear and strong polymorphic band patterns for resistant plant distinguishing it from its susceptible counterpart. The obtained result correlates with 3:1 segregation ratio of single resistant dominant gene inheritance, which depicted ACY as gene-tag functional marker. This marker is currently in use for screening 968 hybrids varieties and one thousand breeding lines of tomato varieties stocked in Jiangsu Green Port Modern Agriculture Development Company (Green Port). So far, ACY has been used to identify 56 hybrids and 51 breeding lines. These newly detected breeding lines were regarded as potential source of resistance for tomato breeding. This work exploited the sequence ofTy-3and subsequently contributed to the development of molecular marker ACY to aid phenotypic selection. We thus recommend this marker to breeders, which is suitable for marker-assisted selection in tomato.

Marker-Assisted Selection in Tomato Breeding

2012 ◽  
Vol 31 (2) ◽  
pp. 93-123 ◽  
Author(s):  
Majid R. Foolad ◽  
Dilip R. Panthee
Keyword(s):  
Marker Assisted Selection ◽  
Tomato Breeding

scholarly journals Deployment of Molecular Markers and Marker-assisted Selection in Tomato Breeding

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1114A-1114
Author(s):  
Majid R. Foolad
Keyword(s):  
Disease Resistance ◽  
Marker Assisted Selection ◽  
Complex Disease ◽  
Morphological Characteristics ◽  
Genetic Maps ◽  
Interspecific Crosses ◽  
Crop Species ◽  
Breeding Programs ◽  
Potential Benefits ◽  
Tomato Breeding

In tomato, Lycopersi conesculentum Mill., currently there are >285 known morphological, physiological and disease resistance markers, 36 isozymes, and >1000 RFLPs, which have been mapped onto the 12 tomato chromosomes. In addition, currently there are >162,000 ESTs, of which ∼3.2% have been mapped. Several tomato genetic maps have been developed, mainly based on interspecific crosses between the cultivated tomato and its related wild species. The markers and maps have been used to locate and tag genes or QTLs for disease resistance and other horticultural characteristics. Such information can be used for various purposes, including marker-assisted selection (MAS) and map-based cloning of desirable genes or QTLs. Many seed companies have adopted using MAS for manipulating genes for a few simple morphological characteristics and several vertical disease resistance traits in tomato. However, MAS is not yet a routine procedure in seed companies for manipulating QTLs although it has been tried for a few complex disease resistance and fruit quality characteristics. In comparison, the use of MAS is less common in public tomato breeding programs, although attempts have been made to transfer QTLs for resistances to a few complex diseases. The potential benefits of marker deployment to plant breeding are undisputed, in particular for pyramiding disease resistance genes. It is expected that in the near future MAS will be routine in many breeding programs, taking advantage of high-resolution markers such as SNPs. For quantitative traits, QTLs must be sought for components of genetic variation before they are applicable to marker-assisted breeding. However, MAS will not be a “silver bullet” solution to every breeding problem or for every crop species.

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