scholarly journals The Function of Fission Yeast Rho1-GEFs in the Control of Cell Growth and Division

2018 ◽  
Author(s):  
Tomás Edreira ◽  
Elvira Manjón ◽  
Yolanda Sánchez
Keyword(s):  
Cell Growth ◽  
Fission Yeast

scholarly journals Genome-wide screen for cell growth regulators in fission yeast

2017 ◽  
Vol 130 (12) ◽  
pp. 2049-2055 ◽  
Author(s):  
Louise Weston ◽  
Jessica Greenwood ◽  
Paul Nurse
Keyword(s):  
Cell Growth ◽  
Fission Yeast ◽  
Growth Regulators ◽  
Genome Wide

scholarly journals Compartmentalized nodes control mitotic entry signaling in fission yeast

2013 ◽  
Vol 24 (12) ◽  
pp. 1872-1881 ◽  
Author(s):  
Lin Deng ◽  
James B. Moseley
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Fission Yeast ◽  
Cell Polarity ◽  
Cell Cycle Progression ◽  
Interaction Network ◽  
Yeast Cells ◽  
Cell Cortex ◽  
Cycle Progression ◽  
Mitotic Entry

Cell cycle progression is coupled to cell growth, but the mechanisms that generate growth-dependent cell cycle progression remain unclear. Fission yeast cells enter into mitosis at a defined size due to the conserved cell cycle kinases Cdr1 and Cdr2, which localize to a set of cortical nodes in the cell middle. Cdr2 is regulated by the cell polarity kinase Pom1, suggesting that interactions between cell polarity proteins and the Cdr1-Cdr2 module might underlie the coordination of cell growth and division. To identify the molecular connections between Cdr1/2 and cell polarity, we performed a comprehensive pairwise yeast two-hybrid screen. From the resulting interaction network, we found that the protein Skb1 interacted with both Cdr1 and the Cdr1 inhibitory target Wee1. Skb1 inhibited mitotic entry through negative regulation of Cdr1 and localized to both the cytoplasm and a novel set of cortical nodes. Skb1 nodes were distinct structures from Cdr1/2 nodes, and artificial targeting of Skb1 to Cdr1/2 nodes delayed entry into mitosis. We propose that the formation of distinct node structures in the cell cortex controls signaling pathways to link cell growth and division.

scholarly journals Cytokinesis-Based Constraints on Polarized Cell Growth in Fission Yeast

PLoS Genetics ◽  
2012 ◽  
Vol 8 (10) ◽  
pp. e1003004 ◽  
Author(s):  
K. Adam Bohnert ◽  
Kathleen L. Gould
Keyword(s):  
Cell Growth ◽  
Fission Yeast ◽  
Polarized Cell Growth

scholarly journals Phosphatases Generate Signal Specificity Downstream of Ssp1 Kinase in Fission Yeast

2017 ◽  
Vol 37 (10) ◽  
Author(s):  
Lin Deng ◽  
Mid Eum Lee ◽  
Katherine L. Schutt ◽  
James B. Moseley
Keyword(s):  
Protein Kinase ◽  
Cell Growth ◽  
Fission Yeast ◽  
Cell Cycle Progression ◽  
Content Type ◽  
Upstream Kinase ◽  
Phosphatase 2A ◽  
Regulatory Input ◽  
Input Cues ◽  
And Migration

ABSTRACT AMPK-related protein kinases (ARKs) coordinate cell growth, proliferation, and migration with environmental status. It is unclear how specific ARKs are activated at specific times. In the fission yeast Schizosaccharomyces pombe, the CaMKK-like protein kinase Ssp1 promotes cell cycle progression by activating the ARK Cdr2 according to cell growth signals. Here, we demonstrate that Ssp1 activates a second ARK, Ssp2/AMPKα, for cell proliferation in low environmental glucose. Ssp1 activates these two related targets by the same biochemical mechanism: direct phosphorylation of a conserved residue in the activation loop (Cdr2-T166 and Ssp2-T189). Despite a shared upstream kinase and similar phosphorylation sites, Cdr2 and Ssp2 have distinct regulatory input cues and distinct functional outputs. We investigated this specificity and found that distinct protein phosphatases counteract Ssp1 activity toward its different substrates. We identified the PP6 family phosphatase Ppe1 as the primary phosphatase for Ssp2-T189 dephosphorylation. The phosphatase inhibitor Sds23 acts upstream of PP6 to regulate Ssp2-T189 phosphorylation in a manner that depends on energy but not on the intact AMPK heterotrimer. In contrast, Cdr2-T166 phosphorylation is regulated by protein phosphatase 2A but not by the Sds23-PP6 pathway. Thus, our study provides a phosphatase-driven mechanism to induce specific physiological responses downstream of a master protein kinase.

scholarly journals S-Adenosylmethionine Synthetase Is Required for Cell Growth, Maintenance of G0 Phase, and Termination of Quiescence in Fission Yeast

iScience ◽  
2018 ◽  
Vol 5 ◽  
pp. 38-51 ◽  
Author(s):  
Takeshi Hayashi ◽  
Takayuki Teruya ◽  
Romanas Chaleckis ◽  
Susumu Morigasaki ◽  
Mitsuhiro Yanagida
Keyword(s):  
Cell Growth ◽  
Fission Yeast ◽  
G0 Phase ◽  
Adenosylmethionine Synthetase

scholarly journals Fission Yeast Cells Grow Approximately Exponentially

2018 ◽  
Author(s):  
Mary Pickering ◽  
Lauren Nicole Hollis ◽  
Edridge D’Souza ◽  
Nicholas Rhind
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Fission Yeast ◽  
Cell Size ◽  
Exponential Growth ◽  
Cell Biology ◽  
Growth Models ◽  
Growth Period ◽  
Yeast Cells ◽  
Yeast Growth

ABSTRACTHow the rate of cell growth is influenced by cell size is a fundamental question of cell biology. The simple model that cell growth is proportional to cell size, based on the proposition that larger cells have proportionally greater synthetic capacity than smaller cells, leads to the predication that the rate of cell growth increases exponentially with cell size. However, other modes of cell growth, including bilinear growth, have been reported. The distinction between exponential and bilinear growth has been explored in particular detail in the fission yeast Schizosaccharomyces pombe. We have revisited the mode of fission yeast cell growth using high-resolution time-lapse microscopy and find, as previously reported, that these two growth models are difficult to distinguish both because of the similarity in shapes between exponential and bilinear curves over the two-fold change in length of a normal cell cycle and because of the substantial biological and experimental noise inherent to these experiments. Therefore, we contrived to have cells grow more than two fold, by holding them in G2 for up to eight hours. Over this extended growth period, in which cells grow up to 5.5-fold, the two growth models diverge to the point that we can confidently exclude bilinear growth as a general model for fission yeast growth. Although the growth we observe is clearly more complicated than predicted by simple exponential growth, we find that exponential growth is a robust approximation of fission yeast growth, both during an unperturbed cell cycle and during extended periods of growth.

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