scholarly journals Biological Control of Fusarium oxysporum in Tomato Seedling Production with Mexican Strains of Trichoderma

Author(s):  
Omar Romero Arenas ◽  
Jesús Francisco López Olguín ◽  
Dionicio Juárez Ramón ◽  
Dora Ma. Sangerman-Jarquín ◽  
Conrado Parraguirre Lezama ◽  
...  
1987 ◽  
Vol 33 (5) ◽  
pp. 349-353 ◽  
Author(s):  
T. C. Paulitz ◽  
C. S. Park ◽  
R. Baker

Nonpathogenic isolates of Fusarium oxysporum were obtained from surface-disinfested, symptomless cucumber roots grown in two raw (nonautoclaved) soils. These isolates were screened for pathogenicity and biological control activity against Fusarium wilt of cucumber in raw soil infested with Fusarium oxysporum f. sp. cucumerinum (F.o.c.). The influence of three isolates effective in inducing suppressiveness and three ineffective isolates on disease incidence over time was tested. The effective isolates reduced the infection rate (R), based on linear regressions of data transformed to loge (1/1 – y). Effective isolate C5 was added to raw soil infested with various inoculum densities of F.o.c. In treatments without C5, the increase in inoculum densities of F.o.c. decreased the incubation period of wilt disease, but there was no significant difference in infection rate among the inoculum density treatments. Isolate C5 reduced the infection rate at all inoculum densities of F.o.c. Various inoculum densities of C5 were added to raw soils infested with 1000 cfu/g of F.o.c. In the first trial, infection rates were reduced only in the treatment with 10 000 cfu/g of C5; in the second trial, infection rates were reduced in treatments with 10 000 and 30 000 cfu/g of C5.


Author(s):  
Tania Ameyally Rios-Hernández ◽  
Alberto Uc-Varguez ◽  
Zahaed Evangelista-Martínez

<em>Fusarium oxysporum</em> causa la pudrición del cormo en gladiolo provocando pérdidas de hasta el 100%. Se seleccionaron aislados de Fusarium a partir de cormos infectados, se identificó morfológica y molecularmente y se seleccionó un aislado a partir de prueba de patogenicidad. Se seleccionó entre 22 aislados de estreptomicetos una cepa que presentó una actividad antagonista del 40% contra <em>Fusarium</em>. Se obtuvo el Extracto Bioactivo (EB) mediante Fermentación en Fase Sólida y se determinó la concentración mínima inhibitoria (MIC) y concentración mínima letal (MLC) por el método de microdilución. Se obtuvo una MIC para el EB de 0.19 mg mL-1 y una MLC de 0.38 mg mL-1, que se confirmó con un ensayo de germinación de microconidios a 8 h, mostrando un porcentaje de inhibición del 17 y 98% para ¼ y ½ de la MIC. Se evaluó el efecto del EB a 1 y 2 MIC’s de concentración contra la pudrición en cormos de gladiolo infectados, obteniendo un efecto protector en los cormos al mantener su dureza después de 15 días, en comparación con el fungicida Carbendazim. Los resultados indican a <em>Streptomyces</em> sp., como un potencial agente de control biológico contra <em>F. oxysporum</em>.


Author(s):  
Castañeda Alvarez Estefania ◽  
Sánchez Leal Ligia

For farmers the use of agrochemicals is the preferred method to control pests and diseases. Considering the market demand for biological control products, the encapsulation could be a competent alternative to current commercial formulations for cellular viability and controlled release. The purpose of this study was to use ionic gelation with sodium alginate, starch and maltodextrin to immobilize Bacillus subtilis and to evaluate the biocontrol effect against Fusarium oxysporum f. sp. lycopersici in vitro. The matrix with a concentration of 2% sodium alginate, 1% starch, and 1% maltodextrin is a suitable method for cellular viability and biological control activity against Fusarium oxysporum f. sp. lycopersici, with a reduction of mycelial growth of 49.6% and a survival rate for Bacillus subtilis of 98.05% (p less than 0.0001).The use of immobilized bacteria as biological control agents are sustainable and effective bio-inputs that could be used at industrial scale and benefit the tomato crops against attack by Fusarium oxysporum f. sp. lycopersici.


2017 ◽  
Vol 4 (11) ◽  
pp. 66-71
Author(s):  
Valmik M. Patil ◽  
◽  
Kishor R. Patole ◽  
Mohan S. Paprikar ◽  
Jaysingh C. Rajput ◽  
...  

2006 ◽  
Vol 3 (1) ◽  
pp. 114-117 ◽  
Author(s):  
Ramandeep Kaur ◽  
Jaspal Kaur ◽  
Rama S. Singh ◽  
C. Alabouvett

1987 ◽  
pp. 63-66 ◽  
Author(s):  
R. Tramier ◽  
A. Antonini ◽  
A. Bettachini

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Arfe Castillo ◽  
Cecirly Puig ◽  
Christian Cumagun

Philippine banana is currently threatened by Fusarium oxysporum f. sp. cubense Tropical Race 4 (FocR4). This study investigated the use of Trichoderma harzianum pre-treated with Glomus spp, as a means of managing Fusarium wilt on young ‘Lakatan’ banana seedlings. Results showed that Glomus applied basally significantly improved banana seedling growth with increased increment in plant height and pseudostem diameter and heavier root weight. The application of Glomus spp. alone offered 100% protection to the ‘Lakatan’ seedlings against FocR4 as indicated by the absence of the wilting symptom. A combination of T. harzianum and Glomus spp. also gave significant effect against Fusarium wilt through delayed disease progression in the seedlings but was not synergistic. Competitive effects were suspected when application of the two biological control agents on banana roots was done simultaneously.


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