scholarly journals Plasma Polymerization for Tissue Engineering Purposes

2018 ◽  
Author(s):  
Gaelle Aziz ◽  
Rouba Ghobeira ◽  
Rino Morent ◽  
Nathalie De Geyter
Keyword(s):  
Tissue Engineering ◽  
Plasma Polymerization

Comparative Effectiveness of Surface Functionalized Poly-ε-Caprolactone Scaffold and β-TCP Mixed PCL Scaffold for Bone Regeneration

2020 ◽  
Vol 20 (9) ◽  
pp. 5349-5355
Author(s):  
Dong-Hwan Yang ◽  
Gwang-Min Heo ◽  
Hong-Ju Park ◽  
Hee-Kyun Oh ◽  
Min-Suk Kook
Keyword(s):  
Tissue Engineering ◽  
Comparative Effectiveness ◽  
Plasma Polymerization ◽  
Oxygen Plasma ◽  
Biological Response ◽  
The Other ◽  
3D Scaffold ◽  
3D Scaffolds ◽  
Chemical Surface ◽  
Proliferation And Differentiation

Physio-chemical surface properties to biomaterial has been attention in tissue engineering due to their properties on cell adhesion, proliferation, and differentiation. The object of this study is to evaluate the preosteoblast biological response on physio-chemical surface-layered 3D PCL scaffold and 3D PCL/β-TCP scaffold. 3D scaffolds were fabricated by FDM 3D printing. Physio-chemical surface of 3D scaffolds were prepared by oxygen plasma and amine plasma-polymerization, respectively. The results of this study demonstrated that amine plasma-treated 3D scaffold on adhesion, proliferation, and osteogenic differentiation of the MC3T3-E1 was significantly increased compared to the other scaffolds.

New replica method with plasma polymerized film by glow discharge

1988 ◽  
Vol 46 ◽  
pp. 414-415
Author(s):  
A. Tanaka ◽  
M. Yamaguchi ◽  
T. Hirano
Keyword(s):  
Power Supply ◽  
Plasma Polymerization ◽  
Vacuum Chamber ◽  
Complex Surface ◽  
Replica Method ◽  
Metal Extraction ◽  
High Voltage Power Supply ◽  
Negative Glow ◽  
Hydrocarbon Gas ◽  
Hydrocarbon Molecules

The plasma polymerization replica method and its apparatus have been devised by Tanaka (1-3). We have published several reports on its application: surface replicas of biological and inorganic specimens, replicas of freeze-fractured tissues and metal-extraction replicas with immunocytochemical markers.The apparatus for plasma polymerization consists of a high voltage power supply, a vacuum chamber containing a hydrocarbon gas (naphthalene, methane, ethylene), and electrodes of an anode disk and a cathode of the specimen base. The surface replication by plasma polymerization in negative glow phase on the cathode was carried out by gassing at 0.05-0.1 Torr and glow discharging at 1.5-3 kV D.C. Ionized hydrocarbon molecules diffused into complex surface configurations and deposited as a three-dimensionally polymerized film of 1050 nm in thickness.The resulting film on the complex surface had uniform thickness and showed no granular texture. Since the film was chemically inert, resistant to heat and mecanically strong, it could be treated with almost any organic or inorganic solvents.

Three-Dimensional Immunoelectron Microscopy of Yeast Cells by a New High-Resolution Replica Method

1985 ◽  
Vol 43 ◽  
pp. 562-563
Author(s):  
Hirano T. ◽  
M. Yamaguchi ◽  
M. Hayashi ◽  
Y. Sekiguchi ◽  
A. Tanaka
Keyword(s):  
High Resolution ◽  
Plasma Polymerization ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Replica Method ◽  
Yeast Cells ◽  
Dynamic Aspect ◽  
Replica Technique ◽  
Gaseous Hydrocarbon ◽  
Transmission Electron

A plasma polymerization film replica method is a new high resolution replica technique devised by Tanaka et al. in 1978. It has been developed for investigation of the three dimensional ultrastructure in biological or nonbiological specimens with the transmission electron microscope. This method is based on direct observation of the single-stage replica film, which was obtained by directly coating on the specimen surface. A plasma polymerization film was deposited by gaseous hydrocarbon monomer in a glow discharge.The present study further developed the freeze fracture method by means of a plasma polymerization film produces a three dimensional replica of chemically untreated cells and provides a clear evidence of fine structure of the yeast plasma membrane, especially the dynamic aspect of the structure of invagination (Figure 1).

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Combination of MRI and 99mTc-NTP 15-5 Scintigraphy for the Assessment of Intervertebral Disk Degeneration and Tissue Engineering

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1319873-s-0032-1319873
Author(s):  
P. Colombier ◽  
J. Clouet ◽  
E. Miot-Noirault ◽  
A. Vidal ◽  
F. Cachin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Intervertebral Disk ◽  
Intervertebral Disk Degeneration ◽  
Disk Degeneration
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