scholarly journals Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

2018 ◽  
Author(s):  
Cigdem Yesildag ◽  
Zhenfang Zhang ◽  
Fang Ren ◽  
Gonzalo de Vicente ◽  
Marga C. Lensen
Keyword(s):  
Gold Nanoparticles ◽  
Cell Adhesion ◽  
Micro Patterning

scholarly journals The Inhibitory Effects of Gold Nanoparticles on VEGF-A-Induced Cell Migration in Choroid-Retina Endothelial Cells

2019 ◽  
Vol 21 (1) ◽  
pp. 109 ◽  
Author(s):  
Chi-Ming Chan ◽  
Chien-Yu Hsiao ◽  
Hsin-Ju Li ◽  
Jia-You Fang ◽  
Der-Chen Chang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Endothelial Cells ◽  
Cell Migration ◽  
Cell Adhesion ◽  
Age Related Macular Degeneration ◽  
Adhesion Assay ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
Viability Assay ◽  
Age Related

Background: Vascular endothelial growth factor (VEGF) is upregulated by hypoxia and is a crucial stimulator for choroidal neovascularization (CNV) in age-related macular degeneration and pathologic myopia, as well as retinal neovascularization in proliferative diabetic retinopathy. Retinal and choroidal endothelial cells play key roles in the development of retinal and CNV, and subsequent fibrosis. At present, the effects of gold nanoparticles (AuNPs) on the VEGF-induced choroid-retina endothelial (RF/6A) cells are still unknown. In our study, we investigated the effects of AuNPs on RF/6A cell viabilities and cell adhesion to fibronectin, a major ECM protein of fibrovascular membrane. Furthermore, the inhibitory effects of AuNPs on RF/6A cell migration induced by VEGF and its signaling were studied. Methods: The cell viability assay was used to determine the viability of cells treated with AuNPs. The migration of RF/6A cells was assessed by the Transwell migration assay. The cell adhesion to fibronectin was examined by an adhesion assay. The VEGF-induced signaling pathways were determined by western blotting. Results: The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay revealed no cytotoxicity of AuNPs on RF/6A cells. AuNPs inhibited VEGF-induced RF/6A cell migration in a concentration-dependent manner but showed no significant effects on RF/6A cell adhesion to fibronectin. Inhibitory effects of AuNPs on VEGF-induced Akt/eNOS were found. Conclusions: These results suggest that AuNPs are an effective inhibitor of VEGF-induced RF/6A cell migration through the Akt/eNOS pathways, but they have no effects on their cell viabilities and cell adhesion to fibronectin.

Femtosecond laser nano/micro patterning of titanium influences mesenchymal stem cell adhesion and commitment

2015 ◽  
Vol 10 (5) ◽  
pp. 055002 ◽  
Author(s):  
Virginie Dumas ◽  
Alain Guignandon ◽  
Laurence Vico ◽  
Cyril Mauclair ◽  
Ximena Zapata ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Mesenchymal Stem Cell ◽  
Femtosecond Laser ◽  
Micro Patterning

scholarly journals Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles

2021 ◽  
Vol 23 (1) ◽  
pp. 260
Author(s):  
Megane Beldjilali Labro ◽  
Rachid Jellali ◽  
Alexander David Brown ◽  
Alejandro Garcia Garcia ◽  
Augustin Lerebours ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gold Nanoparticles ◽  
Electrical Stimulation ◽  
Myogenic Differentiation ◽  
Electrospinning Process ◽  
C2c12 Cells ◽  
Micro Patterning ◽  
Engineered Tissue ◽  
Tunable Mechanical Properties ◽  
Poly Caprolactone

The development of new, viable, and functional engineered tissue is a complex and challenging task. Skeletal muscle constructs have specific requirements as cells are sensitive to the stiffness, geometry of the materials, and biological micro-environment. The aim of this study was thus to design and characterize a multi-scale scaffold and to evaluate it regarding the differentiation process of C2C12 skeletal myoblasts. The significance of the work lies in the microfabrication of lines of polyethylene glycol, on poly(-caprolactone) nanofiber sheets obtained using the electrospinning process, coated or not with gold nanoparticles to act as a potential substrate for electrical stimulation. The differentiation of C2C12 cells was studied over a period of seven days and quantified through both expression of specific genes, and analysis of the myotubes’ alignment and length using confocal microscopy. We demonstrated that our multiscale bio-construct presented tunable mechanical properties and supported the different stages skeletal muscle,as well as improving the parallel orientation of the myotubes with a variation of less than 15°. These scaffolds showed the ability of sustained myogenic differentiation by enhancing the organization of reconstructed skeletal muscle. Moreover, they may be suitable for applications in mechanical and electrical stimulation to mimic the muscle’s physiological functions.

Nano-Fabricated Aligned Spheroid for Cartilage Tissue Engineering

2006 ◽  
Vol 53 ◽  
pp. 67-69
Author(s):  
H. Otsuka ◽  
T. Satomi ◽  
Koji Ueno ◽  
Tetsuya Tateishi
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Gold Surface ◽  
Specific Protein ◽  
Cartilage Tissue ◽  
Patterned Substrate ◽  
Micro Patterning ◽  
Circular Domains

Micropatterned PEGylated substrates with two-dimensional arrays of plasma-etched circular domains (diameter:100 micro-m) were prepared by coating of mercapto-functionalized poly(ethylene glycol) (PEG) on Au surface, followed by plasma-etching through a metal mask pattern with circular holes. The PEGylated region on the patterned substrate works to repel proteins, consequently, inhibits cell adhesion. Then the micro-patterning of bovine articular chondrocytes or rat primary hepatocytes hetero-spheroids underlaid with human umbilical endothelial cells (HUVEC) was achieved on the plasma-etched circular domains, exposing the base gold surface. Obtained results suggested that the efficiency of inhibiting non-specific protein adsorption significantly affects on construction of micro-patterned cell adhesion and hetero-spheroids. The formation of hetero-spheroid thus suggested is significantly modulated by suface properties, particularly non-fouling character of PEG region. These arrayed spheroids is promising materials for tissue and cell-based biosensors (TBB/CBB) as well as tissue engineering technologies.

scholarly journals Neuron Adhesion: Control of Cell Adhesion and Neurite Outgrowth by Patterned Gold Nanoparticles with Tunable Attractive or Repulsive Surface Properties (Small 21/2012)

Small ◽  
2012 ◽  
Vol 8 (21) ◽  
pp. 3226-3226
Author(s):  
Sandra Gilles ◽  
Silke Winter ◽  
Kristin E. Michael ◽  
Simone H. Meffert ◽  
Pinggui Li ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Adhesion ◽  
Neurite Outgrowth ◽  
Surface Properties ◽  
Adhesion Control

scholarly journals The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms

2021 ◽  
Vol 9 ◽  
Author(s):  
Marco Nuno De Canha ◽  
Velaphi Clement Thipe ◽  
Kattesh V. Katti ◽  
Vusani Mandiwana ◽  
Michel Lonji Kalombo ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Adhesion ◽  
Methanol Extract ◽  
Gum Arabic ◽  
Inflammatory Disorder ◽  
Chronic Inflammatory Disorder ◽  
Transmission Electron ◽  
Potent Inhibition ◽  
Cutibacterium Acnes ◽  
Adhesion Activity

The human skin is home to millions of bacteria, fungi, and viruses which form part of a unique microbiome. Commensal microbes, including Cutibacterium acnes can occasionally become opportunistic resulting in the onset of dermatological diseases such as acne. Acne is defined as a chronic inflammatory disorder based on its ability to persist for long periods throughout an individual’s life. The synthesis of gold nanoparticles (AuNPs) was performed using the bottom-up approach by reduction of a gold salt (HAuCl4.3H2O) by the methanol extract (HO-MeOH) and aqueous decoction prepared from the dried aerial parts of Helichrysum odoratissimum (HO-Powder). The HO-MeOH and HO-Powder AuNPs were prepared as unstabilised (−GA) or stabilized (+GA) by the omission or addition of Gum Arabic (GA) as the capping agent. The characterization of the AuNPs was performed using Transmission Electron Microscopy (TEM), dynamic light scattering (DLS), Ultraviolet-Visual spectroscopy (UV-Vis), Thermogravimetric Analysis (TGA), X-Ray Diffraction (XRD) and Zeta-potential. The MBIC50 values for HO-MeOH − GA and HO-MeOH + GA were 1.79 ± 0.78% v/v and 0.22 ± 0.16% v/v, respectively. The HO-Powder AuNPs showed potent inhibition of C. acnes cell adhesion to the 96-well plates. The HO-MeOH − GA and HO-Powder + GA exhibited IC50 of 22.01 ± 6.13% v/v and 11.78 ± 1.78% v/v, respectively. The activity of the AuNPs validated the anti-adhesion activity of the methanol extract in the crude form. The study emphasizes the selectivity of H. odoratissimum AuNPs for the prevention of C. acnes cell adhesion and not antimicrobial activity, which may prevent the emergence of resistant strains of C. acnes through reduced bactericidal or bacteriostatic activity, while targeting mechanisms of pathogenesis.

scholarly journals Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 567
Author(s):  
Ralph El Hallal ◽  
Nana Lyu ◽  
Yuling Wang
Keyword(s):  
Colorectal Cancer ◽  
Gold Nanoparticles ◽  
Epidermal Growth Factor Receptor ◽  
Monoclonal Antibodies ◽  
Cell Adhesion ◽  
Growth Factor ◽  
Cell Surface ◽  
Cell Adhesion Molecule ◽  
Growth Factor Receptor ◽  
Epidermal Growth

Epidermal growth factor receptor (EGFR) is estimated to be overexpressed in 60~80% of colorectal cancer (CRC), which is associated with a poor prognosis. Anti-EGFR targeted monoclonal antibodies (cetuximab and panitumumab) have played an important role in the treatment of metastatic CRC. However, the therapeutic response of anti-EGFR monoclonal antibodies is limited due to multiple resistance mechanisms. With the discovery of new functions for gold nanoparticles (AuNPs), we hypothesize that cetuximab-conjugated AuNPs (cetuximab-AuNPs) will not only improve the cytotoxicity for cancer cells, but also introduce expression change of the related biomarkers on cancer cell surface. In this contribution, we investigated the size-dependent cytotoxicity of cetuximab-AuNPs to CRC cell line (HT-29), while also monitored the expression of cell surface biomarkers in response to treatment with cetuximab and cetuximab-AuNPs. AuNPs with the size of 60 nm showed the highest impact for cell cytotoxicity, which was tested by cell counting kit-8 (CCK-8) assay. Three cell surface biomarkers including epithelial cell adhesion molecule (EpCAM), melanoma cell adhesion molecule (MCAM), and human epidermal growth factor receptor-3 (HER-3) were found to be expressed at higher heterogeneity when cetuximab was conjugated to AuNPs. Both surface-enhanced Raman scattering/spectroscopy (SERS) and flow cytometry demonstrated the correlation of cell surface biomarkers in response to the drug treatment. We thus believe this study provides powerful potential for drug-conjugated AuNPs to enhance cancer prognosis and therapy.

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