scholarly journals CRISPR/Cas9-Facilitated Chromosome Engineering to Model Human Chromosomal Alterations

2018 ◽  
Author(s):  
Zhuo Xing ◽  
Yichen Li ◽  
Annie Pao ◽  
Garrett Kaas ◽  
Y. Eugene Yu
Keyword(s):  
Chromosome Engineering ◽  
Chromosomal Alterations

Correlation between poliomyelitisvirus-reproduction-cycle, chromosomal alterations and lysosomal enzymes

1969 ◽  
Vol 27 (2-4) ◽  
pp. 115-127 ◽  
Author(s):  
H. D. Bartsch ◽  
K. -O. Habermehl ◽  
W. Diefenthal
Keyword(s):  
Lysosomal Enzymes ◽  
Chromosomal Alterations ◽  
Reproduction Cycle

C-banding and fluorescence in situ hybridization studies of the wheat–alien hybrid 'Agrotana'

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 477-481 ◽  
Author(s):  
Jie Xu ◽  
R. L. Conner ◽  
A. Laroche
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic Dna ◽  
Chromosome Engineering ◽  
C Banding ◽  
Chinese Spring Wheat ◽  
Mother Cells ◽  
Alien Chromatin ◽  
Chromosome Segments

'Agrotana', a wheat-alien hybrid (2n = 56), is a potential source of resistance to common root rot, stem rust, wheat streak mosaic virus, and the wheat curl mite. However, the origin of 'Agrotana', reported to be durum wheat × Agropyron trichophorum (pubescent wheatgrass), is uncertain. The objective of this investigation was to determine the chromosome constitution of 'Agrotana' using C-banding and fluorescence in situ hybridization techniques. The F1 hybrid of 'Agrotana' × 'Chinese Spring' wheat showed 7 I + 21 II in 14.9% of the pollen mother cells, evidence of the presence of the A, B, and D genomes in 'Agrotana'. The hybrid had 16 heavily C-banded chromosomes, namely 4A, and 1-7B of wheat, and a translocation that probably involved wheat chromosomes 2A and 2D. In situ hybridization using biotinylated genomic DNA of Ag. trichophorum cv. Greenleaf blocked with CS DNA failed to identify the alien chromosomes in 'Agrotana', indicating that the alien chromosomes were not likely derived from pubescent wheatgrass. In situ hybridization using labelled wheat genomic DNA blocked with 'Agrotana' DNA revealed that 'Agrotana' had 40 wheat, 14 alien, and 2 (a pair) wheat–alien translocated chromosomes. There was no homology between wheat and the alien chromosomes or chromosome segments involved in the wheat–alien recombinant. Two of the seven pairs of alien chromosomes were homoeologous to each other. The ability to identify alien chromatin in wheat using labelled wheat DNA instead of labelled alien DNA will be particularly useful in chromosome engineering of wheat germplasms having alien chromatin of unknown origin.Key words: wheat–alien hybrid, C-banding, fluorescence in situ hybridization, labelled wheat DNA as probe.

Chromosomal alterations and male infertility

2000 ◽  
Vol 23 (10) ◽  
pp. 677-683 ◽  
Author(s):  
A. Antonelli ◽  
L. Gandini ◽  
P. Petrinelli ◽  
L. Marcucci ◽  
R. Elli ◽  
...  
Keyword(s):  
Male Infertility ◽  
Chromosomal Alterations

LOW FREQUENCY OF CHROMOSOMAL ALTERATIONS IN CGH ANALYSIS IN LOW-RISK PAPILLARY BLADDER TUMORS WITH FGFR3 MUTATIONS

2006 ◽  
Vol 5 (14) ◽  
pp. 799
Author(s):  
K. Junker ◽  
J. Van Oers ◽  
E. Zwarthoff ◽  
I. Kania ◽  
J. Schubert ◽  
...  
Keyword(s):  
Low Frequency ◽  
Low Risk ◽  
Bladder Tumors ◽  
Chromosomal Alterations ◽  
Fgfr3 Mutations

scholarly journals Chromosomal alterations in the clonal evolution to the metastatic stage of squamous cell carcinomas of the lung

2000 ◽  
Vol 82 (1) ◽  
pp. 65-73 ◽  
Author(s):  
S Petersen ◽  
M Aninat-Meyer ◽  
K Schlüns ◽  
K Gellert ◽  
M Dietel ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Clonal Evolution ◽  
Squamous Cell Carcinomas ◽  
Chromosomal Alterations

scholarly journals Metabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approach

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Zhenning Liu ◽  
Xue Zhang ◽  
Dengwei Lei ◽  
Bin Qiao ◽  
Guang-Rong Zhao
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
De Novo ◽  
Microbial Cell ◽  
Cell Factory ◽  
Phosphopantetheinyl Transferase ◽  
Chromosome Engineering ◽  
Microbial Cell Factory ◽  
E Coli ◽  
Artificial Pathway

Abstract Background 3-Phenylpropanol with a pleasant odor is widely used in foods, beverages and cosmetics as a fragrance ingredient. It also acts as the precursor and reactant in pharmaceutical and chemical industries. Currently, petroleum-based manufacturing processes of 3-phenypropanol is environmentally unfriendly and unsustainable. In this study, we aim to engineer Escherichia coli as microbial cell factory for de novo production of 3-phenypropanol via retrobiosynthesis approach. Results Aided by in silico retrobiosynthesis analysis, we designed a novel 3-phenylpropanol biosynthetic pathway extending from l-phenylalanine and comprising the phenylalanine ammonia lyase (PAL), enoate reductase (ER), aryl carboxylic acid reductase (CAR) and phosphopantetheinyl transferase (PPTase). We screened the enzymes from plants and microorganisms and reconstructed the artificial pathway for conversion of 3-phenylpropanol from l-phenylalanine. Then we conducted chromosome engineering to increase the supply of precursor l-phenylalanine and combined the upstream l-phenylalanine pathway and downstream 3-phenylpropanol pathway. Finally, we regulated the metabolic pathway strength and optimized fermentation conditions. As a consequence, metabolically engineered E. coli strain produced 847.97 mg/L of 3-phenypropanol at 24 h using glucose-glycerol mixture as co-carbon source. Conclusions We successfully developed an artificial 3-phenylpropanol pathway based on retrobiosynthesis approach, and highest titer of 3-phenylpropanol was achieved in E. coli via systems metabolic engineering strategies including enzyme sources variety, chromosome engineering, metabolic strength balancing and fermentation optimization. This work provides an engineered strain with industrial potential for production of 3-phenylpropanol, and the strategies applied here could be practical for bioengineers to design and reconstruct the microbial cell factory for high valuable chemicals.

scholarly journals Next Generation Cytogenetics in Myeloid Hematological Neoplasms: Detection of CNVs and Translocations

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 3001
Author(s):  
María Chicano ◽  
Diego Carbonell ◽  
Julia Suárez-González ◽  
Sergio Lois ◽  
Mercedes Ballesteros-Culebras ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Lymphoblastic Leukemia ◽  
Copy Number Variations ◽  
Next Generation ◽  
Trisomy 8 ◽  
Chromosomal Alterations ◽  
Hematological Neoplasms ◽  
Myeloid Neoplasm ◽  
Myeloid Neoplasms ◽  
Conventional Cytogenetics

Conventional cytogenetics are the gold standard for the identification of chromosomal alterations recurrent in myeloid neoplasms. Some next-generation sequencing (NGS) panels are designed for the detection of copy number variations (CNV) or translocations; however, their use is far from being widespread. Here we report on the results of a commercial panel including frequent mutations, CNVs and translocations in myeloid neoplasms. Frequent chromosomal alterations were analyzed by NGS in 135 patients with myeloid neoplasms and three with acute lymphoblastic leukemia. NGS analysis was performed using the enrichment-capture Myeloid Neoplasm-GeneSGKit (Sistemas Genómicos, Spain) gene panel including 35 genes for mutational analysis and frequent CNVs and translocations. NGS results were validated with cytogenetics and/or MLPA when possible. A total of 66 frequent alterations included in NGS panel were detected, 48 of them detected by NGS and cytogenetics. Ten of them were observed only by cytogenetics (mainly trisomy 8), and another eight only by NGS (mainly deletion of 12p). Aside from this, 38 secondary CNVs were detected in any of the genes included mainly for mutational analysis. NGS represents a reliable complementary source of information for the analysis of CNVs and translocations. Moreover, NGS could be a useful tool for the detection of alterations not observed by conventional cytogenetics.

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